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The map label for this gene is smpB

Identifier: 30064018

GI number: 30064018

Start: 2748069

End: 2748551

Strand: Direct

Name: smpB

Synonym: S2857

Alternate gene names: 30064018

Gene position: 2748069-2748551 (Clockwise)

Preceding gene: 30064015

Following gene: 30064019

Centisome position: 59.75

GC content: 51.55

Gene sequence:

>483_bases
ATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGA
AGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACA
GCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGTTTGGCGCTAACATCACGCCAATGGCCGTGGCCTCCACGCATGTG
GTGTGCGATCCTACCCGTACCCGCAAGTTACTTCTCAACCAGCGCGAACTGGACTCATTGTACGGTCGCGTCAATCGAGA
AGGCTATACCGTAGTGGCGCTCTCCCTGTACTGGAAAAATGCCTGGTGCAAAGTGAAAATCGGCGTCGCCAAAGGTAAAA
AACAGCACGATAAACGTTCAGATATCAAAGAACGCGAATGGCAGGTGGATAAAGCACGCATCATGAAAAACGCCCACCGT
TAA

Upstream 100 bases:

>100_bases
CGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTATTTTCGATTTCAGATT
ACCGATGATTCACGACGCTT

Downstream 100 bases:

>100_bases
ACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTCCCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCAC
ATCCGACACAAATGTTGCCA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSLRAGKANISDSYVLLRDGEAFLFGANITPMAVASTHV
VCDPTRTRKLLLNQRELDSLYGRVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR

Sequences:

>Translated_160_residues
MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSLRAGKANISDSYVLLRDGEAFLFGANITPMAVASTHV
VCDPTRTRKLLLNQRELDSLYGRVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
>Mature_159_residues
TKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSLRAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVV
CDPTRTRKLLLNQRELDSLYGRVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=160, Percent_Identity=100, Blast_Score=332, Evalue=6e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18269; Mature: 18138

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSLRAGKANISDSYVLLRD
CCCCCCCCCCCEEEEECCCCCCCCCCCHHHCCCEEECCCEEEEEECCCCCCCCCEEEEEC
GEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYGRVNREGYTVVALSLYWKN
CCEEEEECCCCCEEEECEEEEECCCHHHHHHHCHHHHHHHHHHCCCCCEEEEEEEEEECC
AWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
CEEEEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSLRAGKANISDSYVLLRD
CCCCCCCCCCEEEEECCCCCCCCCCCHHHCCCEEECCCEEEEEECCCCCCCCCEEEEEC
GEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYGRVNREGYTVVALSLYWKN
CCEEEEECCCCCEEEECEEEEECCCHHHHHHHCHHHHHHHHHHCCCCCEEEEEEEEEECC
AWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
CEEEEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA