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The map label for this gene is cpsB

Identifier: 30063500

GI number: 30063500

Start: 2111570

End: 2113006

Strand: Reverse

Name: cpsB

Synonym: S2235

Alternate gene names: 30063500

Gene position: 2113006-2111570 (Counterclockwise)

Preceding gene: 30063501

Following gene: 30063499

Centisome position: 45.94

GC content: 55.25

Gene sequence:

>1437_bases
ATGGCGCAGTCGAAACTCTATCCAGTTGTGATGGCAGGTGGCTCCGGTAGCCGTTTATGGCCGCTTTCCCGCGTACTTTA
CCCCAAGCAGTTTTTATGCCTGAAAGGCGATCTCACCATGCTGCAAACCACCATCTGCCGCCTGAACGGCGTGGAGTGCG
AAAGCCCGGTGGTGATTTGCAATGAGCAGCACCGCTTTATTGTCGCGGAACAGCTGCGTCAACTGAACAAACTCACCGAG
AACATTATTCTCGAACCGGCAGGGCGTAACACTGCACCTGCCATTGCACTGGCGGCGCTGGCGGCAAAACGTCATAGCCC
GGAGAGCGACCCGTTAATGCTGGTATTGGCGGCGGATCATGTGATTGCCGATGAAGACGCGTTCCGTGCCGCCGTGCGTA
ATGCCATGCCTTATGCCGAAGCGGGCAAGCTGGTGACCTTTGGCATTGTGCCGGATCTACCAGAAACCGGTTATGGCTAT
ATTCGTCGCGGTGAAGTGTCTGTGGGTGAGCAGGATACGGTGGCCTTTGAAGTGGCGCAGTTTGTCGAAAAACCGAATCT
GGAAACCGCCCAGGCCTATGTAGCAAGCGGCGAATATTACTGGAACAGCGGCATGTTCCTGTTCCGCGCCGGACGCTATC
TCGAAGAACTGAAAAAATATCGCCCGGATATCCTCGATGCCTGCGAAAAAGCGATGAGCGCCGTCGATCCGGATCTCGAT
TTTATTCGTGTGGATGAAGAAGCGTTTCTCGCCTGCCCGGAAGAGTCGGTGGATTACGCGGTCATGGAACCTACGGCAGA
TGCTGTTGTGGTGCCGATGGATGCGGGCTGGAGCGATGTTGGTTCCTGGTCTTCATTATGGGAGATCAGCGCCCACACCG
CCGAGGGCAACGTTTGCCACGGCGATGTGATTAATCACAAAACTGAAAACAGCTATGTGTATGCCGAATCAGGCCTGGTC
ACCACCGTCGGGGTGAAAGATCTGGTAGTGGTGCAGACCAAAGATGCGGTGCTGATTGCCGACCGTAACGCGGTACAGGA
TGTGAAAAAAGTGGTCGAGCAGATCAAAGCCGATGGTCGCCATGAGCATCGGGTGCATCGCGAAGTGTATCGTCCGTGGG
GCAAATATGACTCTATCGACGCGGGCGACCGCTACCAGGTGAAACGCATCACCGTGAAACCGGGCGAGGGTTTGTCGGTA
CAGATGCACCATCACCGCGCGGAACACTGGGTAGTGGTCGCGGGAACGGCAAAAGTCACCATTGATGGTGATATCAAACT
GCTTGGTGAAAACGAGTCCATTTATATTCCGCTGGGGGCGACGCACTGCCTGGAAAACCCGGGGAAAATTCCGCTCGATT
TAATTGAAGTGCGCTCCGGCTCTTATCTCGAAGAGGATGATGTGGTGCGCTTCGCGGATCGCTACGGACGGGTGTAA

Upstream 100 bases:

>100_bases
TGCTGCCCAAACATAACACGGTGGCACGTGAATATGCCGAACGCACGCTCGATAAAGAGAACGTGTTACGTCAATTTATA
AATGATATTCGGGGATAATT

Downstream 100 bases:

>100_bases
ACGTCGCATCAGGCGCTGTTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCGGTTTTGTAGGCCTGATAAG
ACGCGGCAGCGTCGCATCAG

Product: mannose-1-phosphate guanyltransferase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTE
NIILEPAGRNTAPAIALAALAAKRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLD
FIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLV
TTVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSV
QMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV

Sequences:

>Translated_478_residues
MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTE
NIILEPAGRNTAPAIALAALAAKRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLD
FIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLV
TTVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSV
QMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV
>Mature_477_residues
AQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTEN
IILEPAGRNTAPAIALAALAAKRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGYI
RRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLDF
IRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVT
TVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSVQ
MHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV

Specific function: Involved in the biosynthesis of the capsular polysaccharide colanic acid [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=478, Percent_Identity=99.163179916318, Blast_Score=978, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 52957; Mature: 52825

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVIC
CCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCEEEE
NEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAAKRHSPESDPLMLVLAADH
CCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCC
VIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGYIRRGEVSVGEQDTVAFEVAQ
EECCCHHHHHHHHHCCCCHHCCCEEEEECCCCCCCCCCCEEEECEECCCCCCHHHHHHHH
FVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLD
HHHCCCCCHHHHEEECCEEEECCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
FIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCH
EEEECCHHEEECCCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHEEEEEEECCCCCEEE
GDVINHKTENSYVYAESGLVTTVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGR
CHHHCCCCCCCEEEECCCEEEEECCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCC
HEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSVQMHHHRAEHWVVVAGTAKVT
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCEEEEEEEEEEEE
IDGDIKLLGENESIYIPLGATHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV
ECCCEEEEECCCEEEEECCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
AQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVIC
CCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCEEEE
NEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAAKRHSPESDPLMLVLAADH
CCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCC
VIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGYIRRGEVSVGEQDTVAFEVAQ
EECCCHHHHHHHHHCCCCHHCCCEEEEECCCCCCCCCCCEEEECEECCCCCCHHHHHHHH
FVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLD
HHHCCCCCHHHHEEECCEEEECCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
FIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCH
EEEECCHHEEECCCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHEEEEEEECCCCCEEE
GDVINHKTENSYVYAESGLVTTVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGR
CHHHCCCCCCCEEEECCCEEEEECCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCC
HEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSVQMHHHRAEHWVVVAGTAKVT
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCEEEEEEEEEEEE
IDGDIKLLGENESIYIPLGATHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV
ECCCEEEEECCCEEEEECCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1712067; 11677609 [H]