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The map label for this gene is wcaI

Identifier: 30063501

GI number: 30063501

Start: 2113009

End: 2114232

Strand: Reverse

Name: wcaI

Synonym: S2236

Alternate gene names: 30063501

Gene position: 2114232-2113009 (Counterclockwise)

Preceding gene: 30063502

Following gene: 30063500

Centisome position: 45.97

GC content: 55.23

Gene sequence:

>1224_bases
ATGAAAATACTGGTCTACGGCATTAACTACTCGCCGGAGTTAACCGGCATCGGCAAATACACCGGCGAGATGGTGGAATG
GTTGGCGGCACAAGGTCATGAGGTGCGGGTCATTACCGCACCGCCTTACTACCCGCAATGGCAGGTGGGTGAGAACTATT
CCGCCTGGCGCTACAAACGAGAAGAAGGGGCCGCCACGGTGTGGCGCTGCCCGCTGTACGTGCCAAAACAGCCGAGCACC
CTGAAACGCTTGTTGCATCTCGGCAGTTTTGCCGTCAGCAGTTTCTTTCCACTGATGGCGCAACGTCGCTGGAAGCCGGA
TCGCATTATTGGCGTAGTGCCAACGCTGTTTTGCACGCCGGGAATGCGCCTGCTGGCGAAGCTCTCTGGTGCGCGTACCG
TGCTGCATATTCAGGATTACGAAGTGGACGCCATGCTGGGGCTGGGCCTTGCCGGAAAAGGCAAAGGCGGCAAAGTGGCA
CAGCTGGCAACGGCGTTCGAACGTAGCGGACTGCATAACGTCGATAACGTTTCCACGATTTCGCGTTCGATGATGAATAA
AGCCATCGAAAAAGGCGTGGCGGCGGATAACGTCATCTTCTTCCCCAACTGGTCGGAAATTGCCCGTTTTCAGCATGTTG
CAGACGCCGATGTTGATGCCCTTCGTAACCAGCTTGGCCTGCCGGATAACAAAAAAATCATTCTTTACTCCGGCAATATT
GGTGAAAAGCAGGGGCTGGAAAACGTTATTGAAGCTGCCGATCTCCTGCGCGATGAACCGCTGATTTTTGCCATTGTCGG
GCAGGGCGGCGGCAAAGCGCGGCTGGAAAAAATGGCGCAGCAGCGTGGACTGCGCAACATGCAATTTTTCCCGCTGCAAT
CGTATGACGCTTTACCTGCACTGCTGAAGATGGGCGATTGCCATCTGGTGGTGCAAAAACGCGGCGCGGCAGATGCCGTA
TTGCCGTCGAAACTGACCAATATTCTGGCGGTAGGCGGTAACGCGGTGATTACCGCAGAGGCCCACACAGAACTGGGGCA
GCTTTGCGAAACCTTTCCGGGTATTGCGGTTTGCGTAGAACCGGAATCGGTCGAGGCGCTGGTGGCGGGGATTCGTCAGG
CGCTCCTGCTGCCCAAACATAACACGGTGGCACGTGAATATGCCGAACGCACGCTCGATAAAGAGAACGTGTTACGTCAA
TTTATAAATGATATTCGGGGATAA

Upstream 100 bases:

>100_bases
ACCGCTGGCTGACGCCGGACGCGCTGCTCGCCAGTAACGATGTTCACGCTAACAGCCGAGCCTATTTTCTCGCTGAGAAG
CGTGCCGGAGTACCCGGATT

Downstream 100 bases:

>100_bases
TTATGGCGCAGTCGAAACTCTATCCAGTTGTGATGGCAGGTGGCTCCGGTAGCCGTTTATGGCCGCTTTCCCGCGTACTT
TACCCCAAGCAGTTTTTATG

Product: putative glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYYPQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPST
LKRLLHLGSFAVSSFFPLMAQRRWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADVDALRNQLGLPDNKKIILYSGNI
GEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLEKMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAV
LPSKLTNILAVGGNAVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTLDKENVLRQ
FINDIRG

Sequences:

>Translated_407_residues
MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYYPQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPST
LKRLLHLGSFAVSSFFPLMAQRRWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADVDALRNQLGLPDNKKIILYSGNI
GEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLEKMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAV
LPSKLTNILAVGGNAVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTLDKENVLRQ
FINDIRG
>Mature_407_residues
MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYYPQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPST
LKRLLHLGSFAVSSFFPLMAQRRWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADVDALRNQLGLPDNKKIILYSGNI
GEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLEKMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAV
LPSKLTNILAVGGNAVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTLDKENVLRQ
FINDIRG

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788363, Length=407, Percent_Identity=98.7714987714988, Blast_Score=829, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 44803; Mature: 44803

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYYPQWQVGENYSAWRYKR
CEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHEEEC
EEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQRRWKPDRIIGVVPTLFCTP
CCCCEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVAQLATAFERSGLHNVDNVSTI
HHHHHHHHCCCEEEEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
SRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADVDALRNQLGLPDNKKIILYSGNI
HHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEECCC
GEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLEKMAQQRGLRNMQFFPLQSYDALPA
CCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHH
LLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGNAVITAEAHTELGQLCETFPGIAVCVE
HHHCCCEEEEEECCCCCCHHHHHHHHHHEEECCCEEEEEHHHHHHHHHHHHCCCCEEEEC
PESVEALVAGIRQALLLPKHNTVAREYAERTLDKENVLRQFINDIRG
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYYPQWQVGENYSAWRYKR
CEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHEEEC
EEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQRRWKPDRIIGVVPTLFCTP
CCCCEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVAQLATAFERSGLHNVDNVSTI
HHHHHHHHCCCEEEEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
SRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADVDALRNQLGLPDNKKIILYSGNI
HHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEECCC
GEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLEKMAQQRGLRNMQFFPLQSYDALPA
CCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHH
LLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGNAVITAEAHTELGQLCETFPGIAVCVE
HHHCCCEEEEEECCCCCCHHHHHHHHHHEEECCCEEEEEHHHHHHHHHHHHCCCCEEEEC
PESVEALVAGIRQALLLPKHNTVAREYAERTLDKENVLRQFINDIRG
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503 [H]