Click here to switch to the map view.

The map label for this gene is rstA

Identifier: 30063122

GI number: 30063122

Start: 1696434

End: 1697177

Strand: Direct

Name: rstA

Synonym: S1761

Alternate gene names: 30063122

Gene position: 1696434-1697177 (Clockwise)

Preceding gene: 30063120

Following gene: 30063123

Centisome position: 36.88

GC content: 48.52

Gene sequence:

>744_bases
ATGAACACTATCGTATTTGTGGAAGATGATGCGGAAGTCGGTTCACTGATTGCCGCGTACCTGGCAAAACATGATATGCA
GGTTACCGTAGAACCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCA
TGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCT
CTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGTGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGT
TTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACACTGACCAAAGGTCTTCAGGAAACGTCTCTTA
CTCCCTACAAAGCCCTGCATTTCGGCACGTTAACTATCGACCCCATCAACCGCGTAGTCACCCTGGCTAACACTGAAATC
TCGCTCTCGACAGCTGATTTCGAATTATTGTGGGAACTAGCTACGCATGCCGGGCAAATCATGGACCGCGATGCATTGCT
GAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGC
TCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGATAGT
GACAATAAACAATCAGATGACTAA

Upstream 100 bases:

>100_bases
TCATCAGTAGAATACCTGATGAAAACTTGTTTAGAAACGATTGATAGTAAGTAAAAACAGCGCGGTGTATTGTGACGTTT
TTATATCTACCGTGAATGTT

Downstream 100 bases:

>100_bases
TATTTGTTCATCTGAAAAAGAGAACAATTAACGAATTCAATTAATAAGAGCTTATTCAAATTCCAAACCCCCTACATTTG
ATATGAAAATGAGAAAAAAT

Product: DNA-binding transcriptional regulator RstA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRENPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTS
LDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWDS
DNKQSDD

Sequences:

>Translated_247_residues
MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRENPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTS
LDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWDS
DNKQSDD
>Mature_247_residues
MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRENPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTS
LDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWDS
DNKQSDD

Specific function: Member of the two-component regulatory system rstB/rstA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693140, Length=239, Percent_Identity=99.581589958159, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=34.3347639484979, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI87082012, Length=230, Percent_Identity=32.6086956521739, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788394, Length=238, Percent_Identity=32.7731092436975, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1786784, Length=231, Percent_Identity=32.4675324675325, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=32.9059829059829, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1786599, Length=234, Percent_Identity=29.0598290598291, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1790863, Length=233, Percent_Identity=29.6137339055794, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786911, Length=234, Percent_Identity=28.6324786324786, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789402, Length=230, Percent_Identity=30.8695652173913, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1790860, Length=231, Percent_Identity=27.7056277056277, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787229, Length=232, Percent_Identity=29.3103448275862, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787375, Length=243, Percent_Identity=27.9835390946502, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790552, Length=233, Percent_Identity=28.755364806867, Blast_Score=71, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 27580; Mature: 27580

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRENPDLVLLDIMLPGKDG
CCEEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCCC
MTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNE
HHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHCCCC
QATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEISLSTADFELLWELATHAGQI
HHHHHHCHHHCCCCCCCEEEEEEEEECCCCEEEEEECCEEEEEHHHHHHHHHHHHHHHHH
MDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWDS
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEECCCCCC
DNKQSDD
CCCCCCC
>Mature Secondary Structure
MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRENPDLVLLDIMLPGKDG
CCEEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCCC
MTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNE
HHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHCCCC
QATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEISLSTADFELLWELATHAGQI
HHHHHHCHHHCCCCCCCEEEEEEEEECCCCEEEEEECCEEEEEHHHHHHHHHHHHHHHHH
MDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWDS
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEECCCCCC
DNKQSDD
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1495392 [H]