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The map label for this gene is ydgB

Identifier: 30063120

GI number: 30063120

Start: 1695251

End: 1695973

Strand: Direct

Name: ydgB

Synonym: S1759

Alternate gene names: 30063120

Gene position: 1695251-1695973 (Clockwise)

Preceding gene: 30063119

Following gene: 30063122

Centisome position: 36.86

GC content: 50.9

Gene sequence:

>723_bases
ATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCAT
TAATCAAAAGCAACCGGTGATTGTCAGCTATCGGACACACTATCCAGCCATTGATGGACTGATTAATGCAGGTGCGCAGT
GTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTAAAAACCACCCATGGTCTGCGT
GCTATTTTGCATAACGCCAGTGCGTGGATTGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCA
GATCCACGTTAATACCCCATACCTGCTCAACCATGCGCTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATA
TCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATGTTGCGTATGCTGCAAGCAAAGCGGCACTGGAT
AATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAA
TGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGA
TCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTCCCACTTGATGGCGGTCGTCATCTGCGT
TAA

Upstream 100 bases:

>100_bases
AATGGTGCTGATTGGCATGCTGTTAATTGCCTCAGTTCCGGCGACCTGGATGCTGGTGGGATAACGTTCATCCCATCGTT
GGATAGCAAAGGAGATAACG

Downstream 100 bases:

>100_bases
TGCAGCTTTATCCAGCATATAATCAACACCCATGCGCTTATCCCCCAACACGCAACAGAACCAACAAAAGCGGCGGGCAA
GCGCATCATTCCGGTGAAAT

Product: short chain dehydrogenase

Products: NA

Alternate protein names: DHFR [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLR
AILHNASAWIAEKPGAPLADVLACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSFPLDGGRHLR

Sequences:

>Translated_240_residues
MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLR
AILHNASAWIAEKPGAPLADVLACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSFPLDGGRHLR
>Mature_239_residues
GKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRA
ILHNASAWIAEKPGAPLADVLACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALDN
MTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSFPLDGGRHLR

Specific function: Catalyzes the reduction of dihydrofolate to tetrahydrofolate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FolM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787891, Length=240, Percent_Identity=98.75, Blast_Score=496, Evalue=1e-142,
Organism=Escherichia coli, GI2367175, Length=244, Percent_Identity=26.6393442622951, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 26331; Mature: 26199

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLINAGAQCIQADFS
CCCCCCCEEEEECCCCEEHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCHHHEECCCC
TNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVLACMMQIHVNTPYLLNHAL
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCHHHHHHHHHHHHCCCCHHHHHHH
ERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALDNMTRSFARKLAPEVKVNSIA
HHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCC
PSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSFPLDGGRHLR
CEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
GKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLINAGAQCIQADFS
CCCCCCEEEEECCCCEEHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCHHHEECCCC
TNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVLACMMQIHVNTPYLLNHAL
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCHHHHHHHHHHHHCCCCHHHHHHH
ERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALDNMTRSFARKLAPEVKVNSIA
HHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCC
PSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSFPLDGGRHLR
CEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]