Click here to switch to the map view.

The map label for this gene is ldhA

Identifier: 30062875

GI number: 30062875

Start: 1423921

End: 1424910

Strand: Direct

Name: ldhA

Synonym: S1459

Alternate gene names: 30062875

Gene position: 1423921-1424910 (Clockwise)

Preceding gene: 30062866

Following gene: 30062878

Centisome position: 30.96

GC content: 50.2

Gene sequence:

>990_bases
ATGAAACTCGCCGTTTATAGCACAAAACAGTACGACAAGAAGTACCTGCAACAGGTGAACGAGTCCTTTGGCTTTGAGCT
GGAATTTTTTGACTTTCTGCTGACGGAAAAAACCGCTAAAACTGCCAATGGCTGCGAAGCGGTATGTATTTTCGTAAACG
ATGACGGCAGCCACCCGGTGCTGGAAGAGCTGAAAAAGCACGGCGTTAAATATATCGCCCTGCGCTGTGCCGGTTTCAAT
AACGTCGACCTTGATGCGGCAAAAGAACTGGGGCTGAAAGTAGTCCGTGTTCCAGCCTATGATCCAGAGGCCGTTGCTGA
ACACGCCATCGGTATGATGATGACGCTGAACCGCCGTATTCACCGCGCGTATCAGCGTACCCGTGACGCTAACTTCTCTC
TGGAAGGTCTGACCGGCTTTACTATGTATGGCAAAACGGCAGGCGTTATCGGTACCGGTAAAATCGGTGTGGCGATGCTG
CGCATTCTGAAAGGTTTTGGTATGCGTCTGCTGGCGTTCGATCCGTATCCAAGTGCAGCGGCGCTGGAACTCGGTGTGGA
GTATGTCGATCTGCCAACCCTGTTCTCTGAATCAGACGTTATCTCTCTGCACTGCCCGCTGACACCGGAAAACTACCATC
TGTTGAACGAAGCCGCCTTCGATCAGATGAAAAATGGCGTGATGATCGTCAATACCAGTCGCGGTGCATTGATTGATTCT
CAGGCGGCAATTGAAGCGCTGAAAAATCAGAAAATTGGTTCGTTGGGTATGGACGTCTATGAGAACGAACGCGATCTGTT
CTTTGAAGATAAATCCAACGACGTGATCCAGGATGACGTATTCCGTCGCCTGTCTGCCTGCCACAACGTGCTGTTTACCG
GGCACCAGGCATTCCTGACAGCAGAAGCTCTGACCAGTATTTCTCAGACTACGCTGCAAAACTTAAGCAATCTGGAAAAA
GGTGAAACCTGCCCGAACGAACTGGTTTAA

Upstream 100 bases:

>100_bases
ATGAATTTTTCAATATCGCCATGGCTTTCAATTATATTTGAAATTTTGTAAAATATTTTTAGTAGCTTAAATGTGATTCA
ACATCACTGGAGAAAGTCTT

Downstream 100 bases:

>100_bases
TCTTGCAGCTCCCCTGCAATCCAGGGGAGCTGATTCAGATAATCCCCAATGACCTTTCATTCTCTATTCTTAAAATAGTC
CTGAGTCAGAAACTGTAATT

Product: D-lactate dehydrogenase

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFN
NVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAFDQMKNGVMIVNTSRGALIDS
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEK
GETCPNELV

Sequences:

>Translated_329_residues
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFN
NVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAFDQMKNGVMIVNTSRGALIDS
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEK
GETCPNELV
>Mature_329_residues
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFN
NVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAFDQMKNGVMIVNTSRGALIDS
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEK
GETCPNELV

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=200, Percent_Identity=34, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI61743967, Length=252, Percent_Identity=32.5396825396825, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI4557497, Length=252, Percent_Identity=32.5396825396825, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI145580578, Length=252, Percent_Identity=32.5396825396825, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI4557499, Length=252, Percent_Identity=32.5396825396825, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI145580575, Length=248, Percent_Identity=33.0645161290323, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI6912396, Length=201, Percent_Identity=31.8407960199005, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1787645, Length=329, Percent_Identity=99.3920972644377, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI87082289, Length=269, Percent_Identity=27.5092936802974, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789279, Length=232, Percent_Identity=29.7413793103448, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788660, Length=205, Percent_Identity=26.3414634146341, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25147481, Length=278, Percent_Identity=29.136690647482, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17532191, Length=202, Percent_Identity=26.7326732673267, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320925, Length=288, Percent_Identity=29.1666666666667, Blast_Score=115, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6324055, Length=248, Percent_Identity=29.4354838709677, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322116, Length=232, Percent_Identity=31.0344827586207, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=204, Percent_Identity=27.9411764705882, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24646446, Length=258, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24646448, Length=258, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24646452, Length=258, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24646450, Length=258, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI62472511, Length=258, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19921140, Length=241, Percent_Identity=27.8008298755187, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45552429, Length=201, Percent_Identity=29.8507462686567, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28574286, Length=238, Percent_Identity=27.7310924369748, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24585514, Length=201, Percent_Identity=29.8507462686567, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI28574282, Length=201, Percent_Identity=29.8507462686567, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI28574284, Length=201, Percent_Identity=29.8507462686567, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45551003, Length=201, Percent_Identity=29.8507462686567, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28571528, Length=260, Percent_Identity=26.1538461538462, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24585516, Length=199, Percent_Identity=26.6331658291457, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36502; Mature: 36502

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSHPV
CEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHH
LEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRI
HHHHHHCCCEEEEEEECCCCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAMLRILKGFGMRLLAFDPYPSAA
HHHHHHHCCCCCEECCCCEEEEECCCCCEEECCHHHHHHHHHHHHCCCEEEEECCCCCHH
ALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAFDQMKNGVMIVNTSRGALIDS
HHHHCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEECH
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHCCCCCCCCCHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
AEALTSISQTTLQNLSNLEKGETCPNELV
HHHHHHHHHHHHHHHHHCCCCCCCHHCCC
>Mature Secondary Structure
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSHPV
CEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHH
LEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRI
HHHHHHCCCEEEEEEECCCCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAMLRILKGFGMRLLAFDPYPSAA
HHHHHHHCCCCCEECCCCEEEEECCCCCEEECCHHHHHHHHHHHHCCCEEEEECCCCCHH
ALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAFDQMKNGVMIVNTSRGALIDS
HHHHCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEECH
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHCCCCCCCCCHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
AEALTSISQTTLQNLSNLEKGETCPNELV
HHHHHHHHHHHHHHHHHCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]