Click here to switch to the map view.

The map label for this gene is dmsB

Identifier: 30062382

GI number: 30062382

Start: 889634

End: 890251

Strand: Direct

Name: dmsB

Synonym: S0895

Alternate gene names: 30062382

Gene position: 889634-890251 (Clockwise)

Preceding gene: 30062381

Following gene: 30062383

Centisome position: 19.34

GC content: 51.62

Gene sequence:

>618_bases
ATGACAACCCAGTATGGATTTTTTATTGATTCCAGCCGTTGCACCGGTTGCAAAACCTGCGAGCTGGCTTGTAAAGACTA
CAAAGATTTGACGCCAGAAGTCAGTTTCCGCCGCATTTATGAATATGCTGGCGGCGACTGGCAGGAAGATAACGGTGTCT
GGCACCAGAACGTGTTTGCCTACTATCTGTCGATTTCGTGCAACCACTGCGAAGATCCGGCTTGTACTAAAGTCTGCCCG
AGCGGTGCGATGCATAAACGCGAAGATGGTTTTGTTGTGGTCGATGAAGATGTATGCATTGGCTGCCGTTACTGTCATAT
GGCTTGCCCGTATGGCGCACCGCAATATAACGAAACGAAAGGCCATATGACCAAATGCGATGGTTGTTATGACCGTGTTG
CGGAGGGTAAAAAGCCGATCTGTGTTGAATCTTGCCCGCTGCGGGCGCTGGATTTCGGCCCTATCGACGAGCTGCGTAAA
AAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAATATTGTGATCAAACCCAATGC
CAATAGCCGCCCGACCGGGGATACCACTGGCTATCTGGCAAACCCGAAGGAGGTGTGA

Upstream 100 bases:

>100_bases
TGTATCAACGTACTGACCACTCAACGTCCGTCTCCTCTCGCTAAGGGGAATCCGTCACATACAAACCTTGTTCAGGTTGA
AAAGGTGTAAGGAGTAACCG

Downstream 100 bases:

>100_bases
GATGGGAAGTGGATGGCATGAATGGCCGCTGATGATCTTCACGGTCTTCGGGCAATGTGTGGCGGGTGGTTTTATCGTTC
TGGCTTTGGCGCTGCTCAAA

Product: anaerobic dimethyl sulfoxide reductase subunit B

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: DMSO reductase iron-sulfur subunit

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAGGDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCP
SGAMHKREDGFVVVDEDVCIGCRYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV

Sequences:

>Translated_205_residues
MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAGGDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCP
SGAMHKREDGFVVVDEDVCIGCRYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV
>Mature_204_residues
TTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAGGDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPS
GAMHKREDGFVVVDEDVCIGCRYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRKK
HGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV

Specific function: Electron transfer subunit of the terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1787122, Length=205, Percent_Identity=100, Blast_Score=430, Evalue=1e-122,
Organism=Escherichia coli, GI1787872, Length=205, Percent_Identity=94.1463414634146, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1787749, Length=164, Percent_Identity=35.3658536585366, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789370, Length=198, Percent_Identity=33.8383838383838, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI226510944, Length=155, Percent_Identity=38.7096774193548, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790326, Length=161, Percent_Identity=31.6770186335404, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI2367345, Length=188, Percent_Identity=33.5106382978723, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI2367245, Length=163, Percent_Identity=31.9018404907975, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787478, Length=99, Percent_Identity=43.4343434343434, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787740, Length=101, Percent_Identity=41.5841584158416, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI1789067, Length=177, Percent_Identity=32.7683615819209, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI87082179, Length=162, Percent_Identity=31.4814814814815, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1788811, Length=158, Percent_Identity=31.6455696202532, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1787963, Length=162, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI87082180, Length=153, Percent_Identity=28.7581699346405, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DMSB_ECOLI (P18776)

Other databases:

- EMBL:   J03412
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   F64828
- RefSeq:   AP_001525.1
- RefSeq:   NP_415415.1
- ProteinModelPortal:   P18776
- SMR:   P18776
- DIP:   DIP-9453N
- STRING:   P18776
- EnsemblBacteria:   EBESCT00000001415
- EnsemblBacteria:   EBESCT00000001416
- EnsemblBacteria:   EBESCT00000017203
- GeneID:   945507
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0878
- KEGG:   eco:b0895
- EchoBASE:   EB0229
- EcoGene:   EG10233
- eggNOG:   COG0437
- GeneTree:   EBGT00070000031694
- HOGENOM:   HBG726839
- OMA:   CADPACT
- ProtClustDB:   CLSK879846
- BioCyc:   EcoCyc:DMSB-MONOMER
- BioCyc:   MetaCyc:DMSB-MONOMER
- Genevestigator:   P18776
- GO:   GO:0006810
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR014297
- TIGRFAMs:   TIGR02951

Pfam domain/function: PF00037 Fer4

EC number: NA

Molecular weight: Translated: 22869; Mature: 22738

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
9.8 %Cys+Met (Translated Protein)
7.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
9.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAGGDWQEDNGVWHQNVFA
CCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHCEEE
YYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGCRYCHMACPYGAPQYNETK
EEEEEECCCCCCCCHHHCCCCCCCCCCCCCEEEECCCEEECCHHHEEECCCCCCCCCCCC
GHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRKKHGDLAAVAPLPRAHFTKPN
CCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCC
IVIKPNANSRPTGDTTGYLANPKEV
EEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAGGDWQEDNGVWHQNVFA
CCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHCEEE
YYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGCRYCHMACPYGAPQYNETK
EEEEEECCCCCCCCHHHCCCCCCCCCCCCCEEEECCCEEECCHHHEEECCCCCCCCCCCC
GHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRKKHGDLAAVAPLPRAHFTKPN
CCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCC
IVIKPNANSRPTGDTTGYLANPKEV
EEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Iron-Sulfur Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3062312; 8905232; 9278503; 2174699; 1653010