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The map label for this gene is ybcZ

Identifier: 30062020

GI number: 30062020

Start: 487035

End: 488477

Strand: Reverse

Name: ybcZ

Synonym: S0479

Alternate gene names: 30062020

Gene position: 488477-487035 (Counterclockwise)

Preceding gene: 30062021

Following gene: 30062019

Centisome position: 10.62

GC content: 51.91

Gene sequence:

>1443_bases
ATGGTCAGTAAGCCATTTCAGCGCCCGTTTTCGCTGGCAACCCGCCTGACCTTTTTTATCAGCCTGGCCACCATCGCGGC
GTTTTTCGCCTTTGCATGGATCATAATCCACTCAGTAAAAGTGCATTTTGCCGAGCAGGACATTAATGATTTAAAAGAGA
TTAGCGCCACCCTTGAACGGGTACTAAATCACCCTGACGAAACGCAAGCCCGACGCTTAATGACGCTGGAAGATATCGTC
AGTGGTTATTCCAACGTGTTGATTTCCCTGGCAGATAGCCACGGCAAAACGGTGTATCACTCCCCCGGTGCGCCTGATAT
CCGCGAGTTTGCACGTGATGCCATACCCGATAAAGACGCGCGGGGTGGCGAGGTGTATCTCCTTTCCGGCCCGACGATTA
TGATGCCAGGCCACGGTCACGGGCATATGGAACACAGCAACTGGCGGATGATTAACTTGCCGGTTGGCCCGTTGGTGGAC
GGCAAACCGATTTATACGCTCTACATCGCGCTGTCGATCGATTTTCATCTTCATTACATAAATGATTTGATGAATAAACT
TATTATGACCGCATCGGTAATCAGCATCCTGATCGTCTTTATCGTGCTGTTGGCGGTACATAAAGGTCACGCGCCGATCC
GCAGCGTCAGCCGTCAAATCCAGAATATCACCTCGAAAGATCTCGACGTTCGCCTTGACCCGCAGACCGTGCCGATTGAG
CTGGAACAGCTGGTACTGTCGTTCAACCATATGATCGAGCGTATTGAGGATGTCTTTACTCGCCAGTCCAATTTCTCAGC
GGATATCGCCCACGAAATTCGCACACCAATTACTAATCTCATTACGCAAACGGAAATCGCCCTCAGCCAGTCGCGCAGCC
AGAAGGAGCTGGAAGATGTGCTCTACTCTAATCTCGAAGAGCTGACGCGAATGGCGAAAATGGTCAGCGATATGCTGTTT
CTCGCTCAGGCCGATAACAACCAGCTAATCCCCGAAAAGAAAATGCTCAACCTGGCGGATGAAGTCGGCAAAGTGTTCGA
TTTTTTCGAGGCGCTAGCGGAAGATCGCGGCGTGGAGTTGCGGTTTGTTGGCGACAAACGTCAGGTTGCGGGCGATCCGT
TGATGCTGCGTCGGGCGCTAAGCAACCTGCTTTCTAATGCCCTTCGTTATACGCCAACCAGAGAGACAATTGTAGTACGC
TGTCAGACGGTCGATCACCAGGTGCAAGTTTCCGTCGAAAACCCCGGTACGCCCATTGCGCCCGAGCACTTACCGCGATT
GTTTGACCGTTTCTATCGCGTTGACCCCTCCCGCCAGCGAAAAGGTGAAGGTAGCGGTATTGGGCTGGCGATAGTGAAAT
CGATTGTTGTCGCGCATAAAGGCACGGTTGCGGTAACGTCAGATGCGCGGGGGACAAGGTTTGTTATCACATTACCCGCT
TAA

Upstream 100 bases:

>100_bases
GATGTGGCGGTGAAGCTGCTACGCGGCAAAATCGACAACGACTTTGAGCCGAAGCTGATTCAAACCGTGCGCGGCGTGGG
TTACATGCTTGAGGTGCCGG

Downstream 100 bases:

>100_bases
TTATTCAGCAAACGGCAACTTTTATAACCAGTGTAAAAATAACGTGCCGCAATAGTCATACATATTAATGGTAAAAAGCT
GTCACAATTCATAAAAAACC

Product: sensor kinase CusS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTPTRETIVVR
CQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVITLPA

Sequences:

>Translated_480_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTPTRETIVVR
CQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVITLPA
>Mature_480_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTPTRETIVVR
CQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVITLPA

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=480, Percent_Identity=98.125, Blast_Score=963, Evalue=0.0,
Organism=Escherichia coli, GI1788279, Length=294, Percent_Identity=33.6734693877551, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1786600, Length=263, Percent_Identity=33.4600760456274, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790346, Length=332, Percent_Identity=29.8192771084337, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=29.8892988929889, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1787894, Length=311, Percent_Identity=29.5819935691318, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789403, Length=283, Percent_Identity=30.7420494699647, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI145693157, Length=237, Percent_Identity=27.0042194092827, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789808, Length=283, Percent_Identity=27.5618374558304, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1790551, Length=313, Percent_Identity=27.4760383386581, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789149, Length=236, Percent_Identity=27.5423728813559, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=26.3636363636364, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081816, Length=251, Percent_Identity=27.0916334661355, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788713, Length=232, Percent_Identity=28.448275862069, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787374, Length=340, Percent_Identity=22.3529411764706, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1786912, Length=242, Percent_Identity=26.0330578512397, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790861, Length=301, Percent_Identity=25.2491694352159, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082128, Length=296, Percent_Identity=24.6621621621622, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI48994928, Length=240, Percent_Identity=25.8333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788549, Length=223, Percent_Identity=27.8026905829596, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53851; Mature: 53851

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFAEQDINDLKEISATLER
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCHHHHHHHHCCCCCCC
RGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVDGKPIYTLYIALSIDFHLHYI
CCCEEEEEECCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEHHHHHH
NDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHH
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RFVGDKRQVAGDPLMLRRALSNLLSNALRYTPTRETIVVRCQTVDHQVQVSVENPGTPIA
EEECCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCCC
PEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVITLPA
HHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECC
>Mature Secondary Structure
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFAEQDINDLKEISATLER
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCHHHHHHHHCCCCCCC
RGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVDGKPIYTLYIALSIDFHLHYI
CCCEEEEEECCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEHHHHHH
NDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHH
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RFVGDKRQVAGDPLMLRRALSNLLSNALRYTPTRETIVVRCQTVDHQVQVSVENPGTPIA
EEECCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCCC
PEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVITLPA
HHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]