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The map label for this gene is rsmE [H]

Identifier: 29349736

GI number: 29349736

Start: 5701392

End: 5702090

Strand: Reverse

Name: rsmE [H]

Synonym: BT_4328

Alternate gene names: 29349736

Gene position: 5702090-5701392 (Counterclockwise)

Preceding gene: 29349737

Following gene: 29349735

Centisome position: 91.08

GC content: 46.78

Gene sequence:

>699_bases
ATGCACGTATTTTATACTCCCGATATACAGAAAAACTACGAACTTCCGGAAGAGGAAGCACAACATTGTACCCGCGTCCT
GCGGTTGGGTATCGGAGACGAAATCACTCTTACGGACGGTAAGGGACATTTCTATAAAGCAGAAATCACGGTTGCAACCA
ACAAGCGTTGCCTTGTAGCCATCAAAGAAACAATCTTTCAGGAACCGCTCTGGCCGTGTCATCTGCACATTGCCATGGCG
CCGACCAAAAATATGGACCGCAATGAATGGCTTGCCGAGAAAGCTACGGAAATCGGATTTGACGAACTGACTTTTCTCAA
CTGCCGTTTTTCCGAACGCAAGGTGATTAAAACCGAACGGATCGAAAAGATTCTGATATCAGCCATCAAGCAATCTCTTA
AGGCTCGCCTGCCGAAACTCAACGAAATGACGGACTTCGACAAGTTCATTGCACAAGAGTTTCAAGGTCAGAAATTTATT
GCTCACTGTTACGAAGGCGAAAAGCCTCAGCTGAAAAATGTACTGAAAGCAGGAGAAGATGCTCTCGTTCTGATCGGTCC
CGAAGGAGACTTCAGCGAAGAGGAAGTGAAGAAAGCCATCGAATGCGGATTCACTCCCATCAGCTTGGGTAAATCACGGC
TTCGGACAGAGACAGCGGCACTCGTGGCTTGCCACACAATGAACTTATTGAACCAATAA

Upstream 100 bases:

>100_bases
CGAAGAAGCATTGGATCAAGCAATCAAAGACGAGAATTACGAACTGGCTGCCAAGATACGCGACCGGATCAATTCAAGAA
ATCAAAACCACTAACCAATT

Downstream 100 bases:

>100_bases
TAACTTTTAAATAGTATGAGTATGGCAAAGAAAATGATCTCACGGCTCTCGGTACTGGCAGTACTGATTGTGTTTCTGGC
AGCATGTTCCAAACAAACGG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MHVFYTPDIQKNYELPEEEAQHCTRVLRLGIGDEITLTDGKGHFYKAEITVATNKRCLVAIKETIFQEPLWPCHLHIAMA
PTKNMDRNEWLAEKATEIGFDELTFLNCRFSERKVIKTERIEKILISAIKQSLKARLPKLNEMTDFDKFIAQEFQGQKFI
AHCYEGEKPQLKNVLKAGEDALVLIGPEGDFSEEEVKKAIECGFTPISLGKSRLRTETAALVACHTMNLLNQ

Sequences:

>Translated_232_residues
MHVFYTPDIQKNYELPEEEAQHCTRVLRLGIGDEITLTDGKGHFYKAEITVATNKRCLVAIKETIFQEPLWPCHLHIAMA
PTKNMDRNEWLAEKATEIGFDELTFLNCRFSERKVIKTERIEKILISAIKQSLKARLPKLNEMTDFDKFIAQEFQGQKFI
AHCYEGEKPQLKNVLKAGEDALVLIGPEGDFSEEEVKKAIECGFTPISLGKSRLRTETAALVACHTMNLLNQ
>Mature_232_residues
MHVFYTPDIQKNYELPEEEAQHCTRVLRLGIGDEITLTDGKGHFYKAEITVATNKRCLVAIKETIFQEPLWPCHLHIAMA
PTKNMDRNEWLAEKATEIGFDELTFLNCRFSERKVIKTERIEKILISAIKQSLKARLPKLNEMTDFDKFIAQEFQGQKFI
AHCYEGEKPQLKNVLKAGEDALVLIGPEGDFSEEEVKKAIECGFTPISLGKSRLRTETAALVACHTMNLLNQ

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=228, Percent_Identity=32.0175438596491, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26484; Mature: 26484

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVFYTPDIQKNYELPEEEAQHCTRVLRLGIGDEITLTDGKGHFYKAEITVATNKRCLVA
CEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEEECCCEEHHH
IKETIFQEPLWPCHLHIAMAPTKNMDRNEWLAEKATEIGFDELTFLNCRFSERKVIKTER
HHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHH
IEKILISAIKQSLKARLPKLNEMTDFDKFIAQEFQGQKFIAHCYEGEKPQLKNVLKAGED
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCC
ALVLIGPEGDFSEEEVKKAIECGFTPISLGKSRLRTETAALVACHTMNLLNQ
EEEEECCCCCCCHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHVFYTPDIQKNYELPEEEAQHCTRVLRLGIGDEITLTDGKGHFYKAEITVATNKRCLVA
CEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEEECCCEEHHH
IKETIFQEPLWPCHLHIAMAPTKNMDRNEWLAEKATEIGFDELTFLNCRFSERKVIKTER
HHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHH
IEKILISAIKQSLKARLPKLNEMTDFDKFIAQEFQGQKFIAHCYEGEKPQLKNVLKAGED
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCC
ALVLIGPEGDFSEEEVKKAIECGFTPISLGKSRLRTETAALVACHTMNLLNQ
EEEEECCCCCCCHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]