Click here to switch to the map view.

The map label for this gene is gapA [H]

Identifier: 29349671

GI number: 29349671

Start: 5613903

End: 5614913

Strand: Reverse

Name: gapA [H]

Synonym: BT_4263

Alternate gene names: 29349671

Gene position: 5614913-5613903 (Counterclockwise)

Preceding gene: 29349681

Following gene: 29349670

Centisome position: 89.69

GC content: 43.32

Gene sequence:

>1011_bases
ATGATTAAAGTAGGTATTAATGGATTCGGACGTATCGGACGTTTCGTTTTCCGCGCTGCAATGAAAAGAAACGATATTCA
AATCGTAGGTATCAACGACCTTTGCCCGGTAGACTACTTGGCTTACATGCTGAAGTATGACACAATGCACGGTCAATTCG
ACGGTACTATCGAAGCAGATGTTGAAAACAGCAAATTGATCGTTAACGGTCAGGCTATCCGCATCACTGCAGAAAGAAAT
CCGGCTGACTTGAAATGGGATGCTGTAGGTGCTGAATACGTTGTTGAATCTACAGGTTTGTTCTTGAGCAAAGACAAAGC
TCAGGCTCATATCGAAGCTGGTGCAAAATATGTTGTAATGTCAGCTCCTTCTAAAGATGACACTCCGATGTTCGTTTGCG
GTGTAAACGAAAAAACATACGTGAAAGGTACTCAGTTCGTTTCTAACGCTTCTTGTACTACTAACTGTCTGGCTCCTATC
GCTAAAGTATTGAACGACAAGTTCGGTATCCTTGACGGTTTGATGACTACAGTTCACTCTACAACTGCTACTCAGAAAAC
AGTTGACGGTCCTTCTATGAAAGACTGGAGAGGTGGTCGTGCTGCTTCTGGCAACATCATCCCTTCTTCTACTGGTGCTG
CTAAAGCTGTAGGTAAAGTAATCCCGGCTTTGAACGGTAAACTGACTGGTATGTCTATGCGCGTTCCGACTTTGGACGTT
TCTGTAGTTGACTTGACTGTTAACTTGGCTAAACCGGCTACTTACGCTGAAATCTGCGCTGCAATGAAAGAAGCTTCTGA
AGGCGAATTGAAGGGTATTCTGGGTTACACTGAAGATGCAGTAGTTTCTTCTGACTTCTTGGGTGACACTCGTACTTCTA
TCTTCGATGCTAAAGCTGGTATCGCTTTGACTGACACTTTCGTAAAAGTTGTATCTTGGTATGACAACGAAATCGGTTAC
TCTAACAAAGTTCTTGACTTGATCGCTCACATGGCATCAGTTAACGCTTAA

Upstream 100 bases:

>100_bases
AACATTTTTGTAATTTTGCACCGCATTTGAAAATAAAACAAATGTGCGAGCTGAGAGATATTTGAACAAATATTATAAAA
CCCTTAAAAGTTTAAACAAA

Downstream 100 bases:

>100_bases
TTAGAAATCTAAACATATAGGAAACCGCTGCGGGAAACCGTAGCGGTTTTTTTGTCTTCATTTTATATATTGTTAAAAAG
CAAGGTATTATCCGTGGTAT

Product: glyceraldehyde 3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH-A [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MIKVGINGFGRIGRFVFRAAMKRNDIQIVGINDLCPVDYLAYMLKYDTMHGQFDGTIEADVENSKLIVNGQAIRITAERN
PADLKWDAVGAEYVVESTGLFLSKDKAQAHIEAGAKYVVMSAPSKDDTPMFVCGVNEKTYVKGTQFVSNASCTTNCLAPI
AKVLNDKFGILDGLMTTVHSTTATQKTVDGPSMKDWRGGRAASGNIIPSSTGAAKAVGKVIPALNGKLTGMSMRVPTLDV
SVVDLTVNLAKPATYAEICAAMKEASEGELKGILGYTEDAVVSSDFLGDTRTSIFDAKAGIALTDTFVKVVSWYDNEIGY
SNKVLDLIAHMASVNA

Sequences:

>Translated_336_residues
MIKVGINGFGRIGRFVFRAAMKRNDIQIVGINDLCPVDYLAYMLKYDTMHGQFDGTIEADVENSKLIVNGQAIRITAERN
PADLKWDAVGAEYVVESTGLFLSKDKAQAHIEAGAKYVVMSAPSKDDTPMFVCGVNEKTYVKGTQFVSNASCTTNCLAPI
AKVLNDKFGILDGLMTTVHSTTATQKTVDGPSMKDWRGGRAASGNIIPSSTGAAKAVGKVIPALNGKLTGMSMRVPTLDV
SVVDLTVNLAKPATYAEICAAMKEASEGELKGILGYTEDAVVSSDFLGDTRTSIFDAKAGIALTDTFVKVVSWYDNEIGY
SNKVLDLIAHMASVNA
>Mature_336_residues
MIKVGINGFGRIGRFVFRAAMKRNDIQIVGINDLCPVDYLAYMLKYDTMHGQFDGTIEADVENSKLIVNGQAIRITAERN
PADLKWDAVGAEYVVESTGLFLSKDKAQAHIEAGAKYVVMSAPSKDDTPMFVCGVNEKTYVKGTQFVSNASCTTNCLAPI
AKVLNDKFGILDGLMTTVHSTTATQKTVDGPSMKDWRGGRAASGNIIPSSTGAAKAVGKVIPALNGKLTGMSMRVPTLDV
SVVDLTVNLAKPATYAEICAAMKEASEGELKGILGYTEDAVVSSDFLGDTRTSIFDAKAGIALTDTFVKVVSWYDNEIGY
SNKVLDLIAHMASVNA

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=333, Percent_Identity=64.2642642642643, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI7657116, Length=334, Percent_Identity=60.7784431137725, Blast_Score=409, Evalue=1e-114,
Organism=Escherichia coli, GI1788079, Length=331, Percent_Identity=72.809667673716, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1789295, Length=337, Percent_Identity=39.1691394658754, Blast_Score=246, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI32566163, Length=337, Percent_Identity=59.0504451038576, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17568413, Length=337, Percent_Identity=59.0504451038576, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17534677, Length=335, Percent_Identity=59.4029850746269, Blast_Score=402, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17534679, Length=335, Percent_Identity=58.8059701492537, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6322409, Length=333, Percent_Identity=63.6636636636637, Blast_Score=433, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322468, Length=333, Percent_Identity=63.0630630630631, Blast_Score=431, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6321631, Length=333, Percent_Identity=63.0630630630631, Blast_Score=430, Evalue=1e-121,
Organism=Drosophila melanogaster, GI85725000, Length=334, Percent_Identity=63.7724550898204, Blast_Score=432, Evalue=1e-121,
Organism=Drosophila melanogaster, GI22023983, Length=334, Percent_Identity=63.7724550898204, Blast_Score=432, Evalue=1e-121,
Organism=Drosophila melanogaster, GI17933600, Length=334, Percent_Identity=63.4730538922156, Blast_Score=432, Evalue=1e-121,
Organism=Drosophila melanogaster, GI18110149, Length=334, Percent_Identity=63.4730538922156, Blast_Score=432, Evalue=1e-121,
Organism=Drosophila melanogaster, GI19922412, Length=337, Percent_Identity=58.160237388724, Blast_Score=384, Evalue=1e-107,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 35902; Mature: 35902

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVGINGFGRIGRFVFRAAMKRNDIQIVGINDLCPVDYLAYMLKYDTMHGQFDGTIEAD
CEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEC
VENSKLIVNGQAIRITAERNPADLKWDAVGAEYVVESTGLFLSKDKAQAHIEAGAKYVVM
CCCCEEEEECEEEEEEECCCCCCCEEECCCCEEEEECCCCEEECCHHHHHHHCCCEEEEE
SAPSKDDTPMFVCGVNEKTYVKGTQFVSNASCTTNCLAPIAKVLNDKFGILDGLMTTVHS
ECCCCCCCCEEEEECCCCEEECCCCEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHC
TTATQKTVDGPSMKDWRGGRAASGNIIPSSTGAAKAVGKVIPALNGKLTGMSMRVPTLDV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECEEEECCEEEE
SVVDLTVNLAKPATYAEICAAMKEASEGELKGILGYTEDAVVSSDFLGDTRTSIFDAKAG
EEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHCCHHCCCCCHHHHHHCCCC
IALTDTFVKVVSWYDNEIGYSNKVLDLIAHMASVNA
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKVGINGFGRIGRFVFRAAMKRNDIQIVGINDLCPVDYLAYMLKYDTMHGQFDGTIEAD
CEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEC
VENSKLIVNGQAIRITAERNPADLKWDAVGAEYVVESTGLFLSKDKAQAHIEAGAKYVVM
CCCCEEEEECEEEEEEECCCCCCCEEECCCCEEEEECCCCEEECCHHHHHHHCCCEEEEE
SAPSKDDTPMFVCGVNEKTYVKGTQFVSNASCTTNCLAPIAKVLNDKFGILDGLMTTVHS
ECCCCCCCCEEEEECCCCEEECCCCEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHC
TTATQKTVDGPSMKDWRGGRAASGNIIPSSTGAAKAVGKVIPALNGKLTGMSMRVPTLDV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECEEEECCEEEE
SVVDLTVNLAKPATYAEICAAMKEASEGELKGILGYTEDAVVSSDFLGDTRTSIFDAKAG
EEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHCCHHCCCCCHHHHHHCCCC
IALTDTFVKVVSWYDNEIGYSNKVLDLIAHMASVNA
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]