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The map label for this gene is yfkN [H]

Identifier: 29346646

GI number: 29346646

Start: 1537247

End: 1538989

Strand: Reverse

Name: yfkN [H]

Synonym: BT_1236

Alternate gene names: 29346646

Gene position: 1538989-1537247 (Counterclockwise)

Preceding gene: 29346647

Following gene: 29346645

Centisome position: 24.58

GC content: 43.43

Gene sequence:

>1743_bases
ATGAAACGATTTGCATGTGTTTACGTATGGCTTCTTTGCCTCGTGCTCTCAATTGCGGCACAGGAAAAAGTAGTGAAGCT
GAAAATTGTACAGACAAGTGATGTACATGGTAATTATTATCCTTATAATTTTATTACCCGTAAGGATTGGCAGGGCAGTC
TGGCACGAATATACGCTTTTGTGGAGAAAGAACGCGAACAATATAAGGAGAATCTTATTTTACTCGACAACGGCGATATA
TTGCAGGGGCAACCGACTGCCTATTACTATAATTATATAGATACAGTATCACCCCACCTATGCGCGGAAATGATGAATTA
CATGAAATATGATGCCGGAAATATGGGTAATCATGATGTGGAGACAGGCCGAGCCGTGTTCGACCGCTGGATAAATACCT
GTGACTTTCCCGTATTAGGAGCTAATATCATCGATATATCGACCGGAGAACCTCATCTTCCTCCTTATAAGGTCATGGAA
CGTGACGGAGTGAAAATCGTAATCCTTGGAATGATCACCCCTGCTATCCCTGCATGGCTCTCGGAGAACCTGTGGAAGGG
ACTGCGTTTCGACGACATGGAGGAGACTGCACGCAAATGGATGAAGATCATCCGTGAAAAAGAGAATCCGGACTTGATGA
TCGGCTTGTTCCATGCGGGGCAGGAGGCTTTCAAAATGTCCGGCAAGTATAATGAAAACGCTTCTCTGAGTGTGGCGAAG
AATGTCCCCGGATTTGACATTGTACTGATGGGACACGATCATGCGCGCGAATGTAAGAAAGTGATGAATGTGGCAGGTGA
TTCGGTACTGGTCATTGATCCGGCAAGCAACGGAATTGTTCTGTCCAACATCGATGTGACCTTGAAACTGAAAGATGGGA
AAGTGCGCAGCAAAGATATAAAAGGAGTATTGACCGAAACGAAAGATTATGGAATCAGTGAGGACTTTATGAAGAATTTT
GCTCCTCAATATGACACTGTCCGTAACTTTGTCTCTAAAAAGATCGGTACATTCACTGAAAGTATCTCGACCCGTCCTTC
TTTCTTCGGATCGTCTGCTTTTATCGACTTGATCCATACCTTGCAGCTTGAAATCACCGGTGCGGAGATATCCTTTGCGG
CACCGCTTTCATTTGATGCGAAAATAAATAAAGGGGATGTGTTTGTCAGTGATATGTTCAATCTGTATAAGTATGAGAAT
ATGCTATATATGATGACTTTATCCGGAAAAGAAATCAAGGATTATCTGGAAATGTCTTATTTCATGTGGACCAACCGGAT
GAAGTCTCCGGACGATCATCTGCTCTGGTTTAAGGAGAAACGTCGCGAAGGTGCGGAGGACAGAGCTTCTTTCCAAAATT
TCAGCTTCAATTTTGATTCAGCATCGGGAATTATTTATACCGTAGACGTCACCAAGCCGCAAGGGGAAAAGATTACGATT
ATCAGCATGGCAGACGGCAGTCCTTTCCTCATGGATAAGATTTATAAGGTGGCTTTGAACTCTTACCGGGGGAATGGCGG
TGGAGAACTGCTGACGAAAGGATCAGGCATCCCGCAGGAGAAATTGAAAGAGCGTATCATCTTCTCTACGGATAAGGATC
TTCGTTTCTATCTGATGAATTATATAGAAAAGAAAGGTACTATGGACCCGAAGGCATTGAACCAATGGAAATTCGTTCCT
GAAAAGTGGACTGTTCCGGCTGCCGAACGTGATTATAAGTTTTTATTCGGAGATAGTCAATAA

Upstream 100 bases:

>100_bases
AATTGATTGTGCATAAATGGAAAGGGGATATATGGATCGACTCCGAATATACGGGAGGTGCACGGTTCATGTTCTCACAT
CCGCTTAAAATAGAAAAAGA

Downstream 100 bases:

>100_bases
GTAGGCAAAAGTGTTCTTTTTGACAGATAATCTCTCGCCTTAGGTTGAACATCTCATTATTACTGGTATCTTTGTCGCAA
CCAAAAGAGAGAGTGTATGG

Product: 2',3'-cyclic-nucleotide 2'-phosphodiesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MKRFACVYVWLLCLVLSIAAQEKVVKLKIVQTSDVHGNYYPYNFITRKDWQGSLARIYAFVEKEREQYKENLILLDNGDI
LQGQPTAYYYNYIDTVSPHLCAEMMNYMKYDAGNMGNHDVETGRAVFDRWINTCDFPVLGANIIDISTGEPHLPPYKVME
RDGVKIVILGMITPAIPAWLSENLWKGLRFDDMEETARKWMKIIREKENPDLMIGLFHAGQEAFKMSGKYNENASLSVAK
NVPGFDIVLMGHDHARECKKVMNVAGDSVLVIDPASNGIVLSNIDVTLKLKDGKVRSKDIKGVLTETKDYGISEDFMKNF
APQYDTVRNFVSKKIGTFTESISTRPSFFGSSAFIDLIHTLQLEITGAEISFAAPLSFDAKINKGDVFVSDMFNLYKYEN
MLYMMTLSGKEIKDYLEMSYFMWTNRMKSPDDHLLWFKEKRREGAEDRASFQNFSFNFDSASGIIYTVDVTKPQGEKITI
ISMADGSPFLMDKIYKVALNSYRGNGGGELLTKGSGIPQEKLKERIIFSTDKDLRFYLMNYIEKKGTMDPKALNQWKFVP
EKWTVPAAERDYKFLFGDSQ

Sequences:

>Translated_580_residues
MKRFACVYVWLLCLVLSIAAQEKVVKLKIVQTSDVHGNYYPYNFITRKDWQGSLARIYAFVEKEREQYKENLILLDNGDI
LQGQPTAYYYNYIDTVSPHLCAEMMNYMKYDAGNMGNHDVETGRAVFDRWINTCDFPVLGANIIDISTGEPHLPPYKVME
RDGVKIVILGMITPAIPAWLSENLWKGLRFDDMEETARKWMKIIREKENPDLMIGLFHAGQEAFKMSGKYNENASLSVAK
NVPGFDIVLMGHDHARECKKVMNVAGDSVLVIDPASNGIVLSNIDVTLKLKDGKVRSKDIKGVLTETKDYGISEDFMKNF
APQYDTVRNFVSKKIGTFTESISTRPSFFGSSAFIDLIHTLQLEITGAEISFAAPLSFDAKINKGDVFVSDMFNLYKYEN
MLYMMTLSGKEIKDYLEMSYFMWTNRMKSPDDHLLWFKEKRREGAEDRASFQNFSFNFDSASGIIYTVDVTKPQGEKITI
ISMADGSPFLMDKIYKVALNSYRGNGGGELLTKGSGIPQEKLKERIIFSTDKDLRFYLMNYIEKKGTMDPKALNQWKFVP
EKWTVPAAERDYKFLFGDSQ
>Mature_580_residues
MKRFACVYVWLLCLVLSIAAQEKVVKLKIVQTSDVHGNYYPYNFITRKDWQGSLARIYAFVEKEREQYKENLILLDNGDI
LQGQPTAYYYNYIDTVSPHLCAEMMNYMKYDAGNMGNHDVETGRAVFDRWINTCDFPVLGANIIDISTGEPHLPPYKVME
RDGVKIVILGMITPAIPAWLSENLWKGLRFDDMEETARKWMKIIREKENPDLMIGLFHAGQEAFKMSGKYNENASLSVAK
NVPGFDIVLMGHDHARECKKVMNVAGDSVLVIDPASNGIVLSNIDVTLKLKDGKVRSKDIKGVLTETKDYGISEDFMKNF
APQYDTVRNFVSKKIGTFTESISTRPSFFGSSAFIDLIHTLQLEITGAEISFAAPLSFDAKINKGDVFVSDMFNLYKYEN
MLYMMTLSGKEIKDYLEMSYFMWTNRMKSPDDHLLWFKEKRREGAEDRASFQNFSFNFDSASGIIYTVDVTKPQGEKITI
ISMADGSPFLMDKIYKVALNSYRGNGGGELLTKGSGIPQEKLKERIIFSTDKDLRFYLMNYIEKKGTMDPKALNQWKFVP
EKWTVPAAERDYKFLFGDSQ

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=619, Percent_Identity=25.686591276252, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI1786687, Length=252, Percent_Identity=24.2063492063492, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 66283; Mature: 66283

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFACVYVWLLCLVLSIAAQEKVVKLKIVQTSDVHGNYYPYNFITRKDWQGSLARIYAF
CCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHH
VEKEREQYKENLILLDNGDILQGQPTAYYYNYIDTVSPHLCAEMMNYMKYDAGNMGNHDV
HHHHHHHHHCCEEEEECCCEECCCCCEEEEECHHCCCHHHHHHHHHHHHCCCCCCCCCCC
ETGRAVFDRWINTCDFPVLGANIIDISTGEPHLPPYKVMERDGVKIVILGMITPAIPAWL
HHHHHHHHHHHCCCCCCEECCEEEEECCCCCCCCCHHHHCCCCCEEEEEECHHHHHHHHH
SENLWKGLRFDDMEETARKWMKIIREKENPDLMIGLFHAGQEAFKMSGKYNENASLSVAK
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHCCCCCCCCCCEEEEC
NVPGFDIVLMGHDHARECKKVMNVAGDSVLVIDPASNGIVLSNIDVTLKLKDGKVRSKDI
CCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECEEEEEEECCCCCCCHH
KGVLTETKDYGISEDFMKNFAPQYDTVRNFVSKKIGTFTESISTRPSFFGSSAFIDLIHT
HHHEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHEE
LQLEITGAEISFAAPLSFDAKINKGDVFVSDMFNLYKYENMLYMMTLSGKEIKDYLEMSY
EEEEEECCEEEEECCCEECCCCCCCCEEHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHH
FMWTNRMKSPDDHLLWFKEKRREGAEDRASFQNFSFNFDSASGIIYTVDVTKPQGEKITI
HHHHCCCCCCCCCEEEEHHHHCCCCHHHHHHCCCEEECCCCCCEEEEEEECCCCCCEEEE
ISMADGSPFLMDKIYKVALNSYRGNGGGELLTKGSGIPQEKLKERIIFSTDKDLRFYLMN
EEECCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHEEECCCCCHHHHHHH
YIEKKGTMDPKALNQWKFVPEKWTVPAAERDYKFLFGDSQ
HHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MKRFACVYVWLLCLVLSIAAQEKVVKLKIVQTSDVHGNYYPYNFITRKDWQGSLARIYAF
CCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHH
VEKEREQYKENLILLDNGDILQGQPTAYYYNYIDTVSPHLCAEMMNYMKYDAGNMGNHDV
HHHHHHHHHCCEEEEECCCEECCCCCEEEEECHHCCCHHHHHHHHHHHHCCCCCCCCCCC
ETGRAVFDRWINTCDFPVLGANIIDISTGEPHLPPYKVMERDGVKIVILGMITPAIPAWL
HHHHHHHHHHHCCCCCCEECCEEEEECCCCCCCCCHHHHCCCCCEEEEEECHHHHHHHHH
SENLWKGLRFDDMEETARKWMKIIREKENPDLMIGLFHAGQEAFKMSGKYNENASLSVAK
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHCCCCCCCCCCEEEEC
NVPGFDIVLMGHDHARECKKVMNVAGDSVLVIDPASNGIVLSNIDVTLKLKDGKVRSKDI
CCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECEEEEEEECCCCCCCHH
KGVLTETKDYGISEDFMKNFAPQYDTVRNFVSKKIGTFTESISTRPSFFGSSAFIDLIHT
HHHEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHEE
LQLEITGAEISFAAPLSFDAKINKGDVFVSDMFNLYKYENMLYMMTLSGKEIKDYLEMSY
EEEEEECCEEEEECCCEECCCCCCCCEEHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHH
FMWTNRMKSPDDHLLWFKEKRREGAEDRASFQNFSFNFDSASGIIYTVDVTKPQGEKITI
HHHHCCCCCCCCCEEEEHHHHCCCCHHHHHHCCCEEECCCCCCEEEEEEECCCCCCEEEE
ISMADGSPFLMDKIYKVALNSYRGNGGGELLTKGSGIPQEKLKERIIFSTDKDLRFYLMN
EEECCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHEEECCCCCHHHHHHH
YIEKKGTMDPKALNQWKFVPEKWTVPAAERDYKFLFGDSQ
HHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]