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The map label for this gene is gacS [H]

Identifier: 29346647

GI number: 29346647

Start: 1538986

End: 1540911

Strand: Reverse

Name: gacS [H]

Synonym: BT_1237

Alternate gene names: 29346647

Gene position: 1540911-1538986 (Counterclockwise)

Preceding gene: 29346649

Following gene: 29346646

Centisome position: 24.61

GC content: 48.18

Gene sequence:

>1926_bases
ATGATGAGATATATAGGGTGCCTGTTTATCTGTTTGCTATGGCTTTTCCGGGCCACAGAGTTATTTGCTGTCTCTAACGA
TCAGAAACCCATCCTGATTATCTGCTCCTACAATCCCGCCGCTCATCAAACTTCGGTCACTATCTCCGACTATATGGAAG
AATATAACAAACTGGGAGGGAAACGCGATATTATCATTGAAAACATGAACTGCAAAAGTTTCTCCGAAGCCCCGCTTTGG
AGCGGTATGATGACGCAGATTCTTTCTAAATATCAAGGGGAGAAGCATCCGGCGCAAATTATTCTGTTGGGACAGGAAGC
GTGGGCGGCGTATCTGTCGCAACGGGATTCGATACAGGTGAAGGTGCCGGTGATGTGTAGTCTCGTCAGCAGCAACATCG
TCATACTGCCTGAGGATACGGTTGCCGGACTGGACGCATGGATGCCGGAATCGGTCGATCTCTTTACCGACCACATGAAT
ATACCGGAATTGAAGTCGGGATTTATCAATCAGTACAATATAGAAGATAATGTCCGGATGATCAAGGCGTTTTATCCGAA
GACGGAACACATCGCTTTTATTTCGGACAATACTTACGGCGGCGTCACCATGCAGGCACTGGTGCGGAAAGAAATGAAGA
AGTTTCCCGAAATCGACCTGATCCTGATGGACGGCCGGCGGCATACTATCTATACAATTGTGGAAGAGTTGCGTCATTTA
CCGGAGAATACGGTGATCATGGTAGGCACGTGGCGTGTGGATATGAACGAAGGTTATTTCATGCGGAATGCTACCTATGC
CATGATGGAGGTTACTCCGACCATTCCGACTTTTACTCCCTCATCGGTGAGTCTGGGGTATTGGGCGATCGGCGGAGTGC
TGCCCGATTACCGGAAACTGGGGATGGACATGGCATTGGCATCCGTACAGATTGATCGATATCCGGCGGATGAGCAGCAG
CACCTTTCCGTGATTGGCAGCAAGGCCGTACTGGACAGCCGGAAGGTGAAGGAGTGGGGACTGGACCCGGACATCCTTCC
TTTTGATGTGCAGATTATTAATCAGACCGTATCATTCTATCAGCAATATACTTATCAGATTTGGTCGGCATGTGCGCTGG
TTGTCATCCTTGTGCTGGGGTTGTGCATCTCGCTCTTCTACTATTTCCGCACCAAACGTCTGAAGGACGATCTGCTGAAG
TCGGAAAAAGACCTGCGGGTGGCAAAAGACCGTGCAGAAGAATCAAACCGCCTGAAAAGTGCTTTCCTCGCCAATATGAG
CCATGAGATACGTACGCCTTTAAACTCCATTGTAGGTTTCTCGGATGTGCTCGCTGTGGGTGGCAATACGGAAGAAGAAC
AGCAGACCTATTATCAGATTATCAAGACAAATTCGGATTTGTTGCTTCGCCTGATCAATGATATTCTTGATCTCTCACGC
CTGGAAGCGAACAGGGTCACGTTGACTTGGGAAGAGTGTGACGTGGTGCAGCTATGCAGGCAGGTGGTGGCATCTGTCAG
CGTTTCACGGCAGTCCGGCAATCAGTTCCTGTTTGTCAGCGATTATGAGTCGTTTCGGATGACGACAGACATACAGCGTA
TGCAGCAGGTGATTATCAATCTGATGTCTAATGCGGATAAGTTCACCAGGAAAGGGCAGATAACACTGGAATTTTCGGTG
AATGAAGAGACGGAGATGGCTGTATTCTCTGTGACGGATACAGGGTGCGGCATCCCGAAAGAGAAACAGAAACTGGTATT
CGAGCGCTTCGAGAAGCTGAACGAATATGCCCAGGGAACAGGTTTGGGATTATCTATCTGTAAATTGATTGTGCATAAAT
GGAAAGGGGATATATGGATCGACTCCGAATATACGGGAGGTGCACGGTTCATGTTCTCACATCCGCTTAAAATAGAAAAA
GAATGA

Upstream 100 bases:

>100_bases
ATGAATGAGCGACAAAAATACACATAAATCTGTTATAGCCGACAAGTTTTAGTATATTTGCAAGCTAAAATGAGATAGTA
ACACTTCAAAACTTACGAGT

Downstream 100 bases:

>100_bases
AACGATTTGCATGTGTTTACGTATGGCTTCTTTGCCTCGTGCTCTCAATTGCGGCACAGGAAAAAGTAGTGAAGCTGAAA
ATTGTACAGACAAGTGATGT

Product: two-component system sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MMRYIGCLFICLLWLFRATELFAVSNDQKPILIICSYNPAAHQTSVTISDYMEEYNKLGGKRDIIIENMNCKSFSEAPLW
SGMMTQILSKYQGEKHPAQIILLGQEAWAAYLSQRDSIQVKVPVMCSLVSSNIVILPEDTVAGLDAWMPESVDLFTDHMN
IPELKSGFINQYNIEDNVRMIKAFYPKTEHIAFISDNTYGGVTMQALVRKEMKKFPEIDLILMDGRRHTIYTIVEELRHL
PENTVIMVGTWRVDMNEGYFMRNATYAMMEVTPTIPTFTPSSVSLGYWAIGGVLPDYRKLGMDMALASVQIDRYPADEQQ
HLSVIGSKAVLDSRKVKEWGLDPDILPFDVQIINQTVSFYQQYTYQIWSACALVVILVLGLCISLFYYFRTKRLKDDLLK
SEKDLRVAKDRAEESNRLKSAFLANMSHEIRTPLNSIVGFSDVLAVGGNTEEEQQTYYQIIKTNSDLLLRLINDILDLSR
LEANRVTLTWEECDVVQLCRQVVASVSVSRQSGNQFLFVSDYESFRMTTDIQRMQQVIINLMSNADKFTRKGQITLEFSV
NEETEMAVFSVTDTGCGIPKEKQKLVFERFEKLNEYAQGTGLGLSICKLIVHKWKGDIWIDSEYTGGARFMFSHPLKIEK
E

Sequences:

>Translated_641_residues
MMRYIGCLFICLLWLFRATELFAVSNDQKPILIICSYNPAAHQTSVTISDYMEEYNKLGGKRDIIIENMNCKSFSEAPLW
SGMMTQILSKYQGEKHPAQIILLGQEAWAAYLSQRDSIQVKVPVMCSLVSSNIVILPEDTVAGLDAWMPESVDLFTDHMN
IPELKSGFINQYNIEDNVRMIKAFYPKTEHIAFISDNTYGGVTMQALVRKEMKKFPEIDLILMDGRRHTIYTIVEELRHL
PENTVIMVGTWRVDMNEGYFMRNATYAMMEVTPTIPTFTPSSVSLGYWAIGGVLPDYRKLGMDMALASVQIDRYPADEQQ
HLSVIGSKAVLDSRKVKEWGLDPDILPFDVQIINQTVSFYQQYTYQIWSACALVVILVLGLCISLFYYFRTKRLKDDLLK
SEKDLRVAKDRAEESNRLKSAFLANMSHEIRTPLNSIVGFSDVLAVGGNTEEEQQTYYQIIKTNSDLLLRLINDILDLSR
LEANRVTLTWEECDVVQLCRQVVASVSVSRQSGNQFLFVSDYESFRMTTDIQRMQQVIINLMSNADKFTRKGQITLEFSV
NEETEMAVFSVTDTGCGIPKEKQKLVFERFEKLNEYAQGTGLGLSICKLIVHKWKGDIWIDSEYTGGARFMFSHPLKIEK
E
>Mature_641_residues
MMRYIGCLFICLLWLFRATELFAVSNDQKPILIICSYNPAAHQTSVTISDYMEEYNKLGGKRDIIIENMNCKSFSEAPLW
SGMMTQILSKYQGEKHPAQIILLGQEAWAAYLSQRDSIQVKVPVMCSLVSSNIVILPEDTVAGLDAWMPESVDLFTDHMN
IPELKSGFINQYNIEDNVRMIKAFYPKTEHIAFISDNTYGGVTMQALVRKEMKKFPEIDLILMDGRRHTIYTIVEELRHL
PENTVIMVGTWRVDMNEGYFMRNATYAMMEVTPTIPTFTPSSVSLGYWAIGGVLPDYRKLGMDMALASVQIDRYPADEQQ
HLSVIGSKAVLDSRKVKEWGLDPDILPFDVQIINQTVSFYQQYTYQIWSACALVVILVLGLCISLFYYFRTKRLKDDLLK
SEKDLRVAKDRAEESNRLKSAFLANMSHEIRTPLNSIVGFSDVLAVGGNTEEEQQTYYQIIKTNSDLLLRLINDILDLSR
LEANRVTLTWEECDVVQLCRQVVASVSVSRQSGNQFLFVSDYESFRMTTDIQRMQQVIINLMSNADKFTRKGQITLEFSV
NEETEMAVFSVTDTGCGIPKEKQKLVFERFEKLNEYAQGTGLGLSICKLIVHKWKGDIWIDSEYTGGARFMFSHPLKIEK
E

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=239, Percent_Identity=35.5648535564854, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1788713, Length=244, Percent_Identity=31.5573770491803, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI145693157, Length=247, Percent_Identity=34.412955465587, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI48994928, Length=232, Percent_Identity=31.4655172413793, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI87081816, Length=238, Percent_Identity=33.6134453781513, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1786912, Length=252, Percent_Identity=28.1746031746032, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788549, Length=231, Percent_Identity=26.4069264069264, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790436, Length=276, Percent_Identity=26.8115942028986, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786600, Length=242, Percent_Identity=28.5123966942149, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788393, Length=247, Percent_Identity=23.8866396761134, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87082128, Length=224, Percent_Identity=27.2321428571429, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1790861, Length=205, Percent_Identity=28.780487804878, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1787894, Length=225, Percent_Identity=28, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1786783, Length=237, Percent_Identity=24.4725738396624, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 73289; Mature: 73289

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRYIGCLFICLLWLFRATELFAVSNDQKPILIICSYNPAAHQTSVTISDYMEEYNKLGG
CCHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHCCC
KRDIIIENMNCKSFSEAPLWSGMMTQILSKYQGEKHPAQIILLGQEAWAAYLSQRDSIQV
CCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCCCCCEEE
KVPVMCSLVSSNIVILPEDTVAGLDAWMPESVDLFTDHMNIPELKSGFINQYNIEDNVRM
EHHHHHHHHCCCEEEEECCCHHCHHHCCCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCEE
IKAFYPKTEHIAFISDNTYGGVTMQALVRKEMKKFPEIDLILMDGRRHTIYTIVEELRHL
HHEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHC
PENTVIMVGTWRVDMNEGYFMRNATYAMMEVTPTIPTFTPSSVSLGYWAIGGVLPDYRKL
CCCCEEEEEEEEEECCCCEEEECCEEEEEEEECCCCCCCCCCCCCCHHHHCCCCHHHHHH
GMDMALASVQIDRYPADEQQHLSVIGSKAVLDSRKVKEWGLDPDILPFDVQIINQTVSFY
CHHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHH
QQYTYQIWSACALVVILVLGLCISLFYYFRTKRLKDDLLKSEKDLRVAKDRAEESNRLKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AFLANMSHEIRTPLNSIVGFSDVLAVGGNTEEEQQTYYQIIKTNSDLLLRLINDILDLSR
HHHHHCHHHHHCCHHHHCCHHHHHEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LEANRVTLTWEECDVVQLCRQVVASVSVSRQSGNQFLFVSDYESFRMTTDIQRMQQVIIN
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
LMSNADKFTRKGQITLEFSVNEETEMAVFSVTDTGCGIPKEKQKLVFERFEKLNEYAQGT
HHHCCHHHCCCCEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
GLGLSICKLIVHKWKGDIWIDSEYTGGARFMFSHPLKIEKE
CCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCEEECCC
>Mature Secondary Structure
MMRYIGCLFICLLWLFRATELFAVSNDQKPILIICSYNPAAHQTSVTISDYMEEYNKLGG
CCHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHCCC
KRDIIIENMNCKSFSEAPLWSGMMTQILSKYQGEKHPAQIILLGQEAWAAYLSQRDSIQV
CCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCCCCCEEE
KVPVMCSLVSSNIVILPEDTVAGLDAWMPESVDLFTDHMNIPELKSGFINQYNIEDNVRM
EHHHHHHHHCCCEEEEECCCHHCHHHCCCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCEE
IKAFYPKTEHIAFISDNTYGGVTMQALVRKEMKKFPEIDLILMDGRRHTIYTIVEELRHL
HHEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHC
PENTVIMVGTWRVDMNEGYFMRNATYAMMEVTPTIPTFTPSSVSLGYWAIGGVLPDYRKL
CCCCEEEEEEEEEECCCCEEEECCEEEEEEEECCCCCCCCCCCCCCHHHHCCCCHHHHHH
GMDMALASVQIDRYPADEQQHLSVIGSKAVLDSRKVKEWGLDPDILPFDVQIINQTVSFY
CHHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHH
QQYTYQIWSACALVVILVLGLCISLFYYFRTKRLKDDLLKSEKDLRVAKDRAEESNRLKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AFLANMSHEIRTPLNSIVGFSDVLAVGGNTEEEQQTYYQIIKTNSDLLLRLINDILDLSR
HHHHHCHHHHHCCHHHHCCHHHHHEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LEANRVTLTWEECDVVQLCRQVVASVSVSRQSGNQFLFVSDYESFRMTTDIQRMQQVIIN
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
LMSNADKFTRKGQITLEFSVNEETEMAVFSVTDTGCGIPKEKQKLVFERFEKLNEYAQGT
HHHCCHHHCCCCEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
GLGLSICKLIVHKWKGDIWIDSEYTGGARFMFSHPLKIEKE
CCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]