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The map label for this gene is pgl [H]

Identifier: 29346428

GI number: 29346428

Start: 1272083

End: 1273420

Strand: Reverse

Name: pgl [H]

Synonym: BT_1018

Alternate gene names: 29346428

Gene position: 1273420-1272083 (Counterclockwise)

Preceding gene: 29346429

Following gene: 29346427

Centisome position: 20.34

GC content: 43.2

Gene sequence:

>1338_bases
ATGAATTTACGAATCACTCTTTTGGCTTTTCTTTGCCTTTGCGCAACAGCGATCCTTCGTGCCGAACGCGTTGATATGTT
GAAAGCGGGTGCAAAAGCAAATGGGAAAGCTCTCAATACAAAGCTGATAAATTCAACGATTGACCGTTTGAATCGTGGAG
GCGGAGGTACGCTCTTTTTTCCTGCCGGAACGTATCTGACGGGAAGTATCCATCTGAAAAGTAACATTACACTGGAACTG
GAAGCCGGAGCTACCCTGCTTTTTTCAGACAACTTTGATGATTATCTTCCTTTTGTAGAAGTACGTCACGAAGGAGTCAT
GATGAAAAGTTTCCAGCCGTTAATCTATGCGGTTGATGCGGAAAATATTACTATTAAGGGAGAAGGTACACTGGATGGTC
AAGGTAAGAAGTGGTGGATGGAGTTCTTTCGTGTAATGATTGACCTAAAAGATAATGGTATGCGTGATATTAATAAATAT
CAGCCGATGTGGGATGCTCAGAATGATACGACTGCCATCTATGCAGAGACGAATAAAGATTATGTCAGCACTTTGCAGCG
TCGTTTTTTCCGCCCTCCGTTCATTCAGCCTGTTCGTTGCAAGAAGGTGAAGATTGAAGGAGTGAAAATTGTCAATTCTC
CTTTCTGGACTGTTAATCCGGAGTTTTGTGATAATGTGACCATCAAAGGGATCACAATAAATAATGTCCCTTCTCCCAAC
ACGGATGGAATCAATCCTGAATCCTGCCGGAATGTACATATCAGTGATTGCCATATTTCTGTAGGCGATGACTGCATTAC
GATTAAATCCGGAAGAGACGCACAAGCCCGCCGCTTGGGAGTACCGTGTGAGAATATTACTATTACCAATTGTACGATGC
TTTCCGGTCATGGTGGTGTAGTGATCGGCAGTGAAATGAGTGGTAGTGTGCGTAAGGTCACGATCTCTAACTGTGTATTT
GACGGAACCGATCGTGGTATCCGCATCAAGTCAACCCGTGGAAGGGGAGGAGTCGTAGAGGATATCCGTGTCAGTAATAT
CGTAATGAGTAACATCAAGCAGGAGGCTGTCGTCTTGAATCTGAAATACAGCCAAATGCCTGCAGAAGCAAAGAGTGAGC
GTACTCCGATATTTCGTAATGTTCATATCAGCGGTATGACTGTGACGGATGTAAAGACTCCGATTAAAATAGTAGGACTG
GAAGAAGCTCCGATTTCTGACATTGTATTGCGTGACATCCATATTCAGGGAGCCAGACAGAAGTGTATCTTTGAAGATTG
TGAACGTATCACTATGGATGACGTAATTATTAACGGTGAAGAAATGAAACTGAAATAA

Upstream 100 bases:

>100_bases
TCCGGGTGAAAAAGAACCGAAAGAAGTACAAAGCGGGACATATAAGTTTGTTGCTGCCGAATAAGATATAAAAAAGAAGA
ACTATTTAATAATATAGAAT

Downstream 100 bases:

>100_bases
ACCTCTTGGGAGGTTACATCTTTATAAATATAAGAAAATGAAACGAATCCTTTTCTCATTGCTTCTCGCTGCATCTCTTT
CGGCAGAAGCACAGACGCAG

Product: polygalacturonase (Pectinase)

Products: NA

Alternate protein names: PGL; Pectinase [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MNLRITLLAFLCLCATAILRAERVDMLKAGAKANGKALNTKLINSTIDRLNRGGGGTLFFPAGTYLTGSIHLKSNITLEL
EAGATLLFSDNFDDYLPFVEVRHEGVMMKSFQPLIYAVDAENITIKGEGTLDGQGKKWWMEFFRVMIDLKDNGMRDINKY
QPMWDAQNDTTAIYAETNKDYVSTLQRRFFRPPFIQPVRCKKVKIEGVKIVNSPFWTVNPEFCDNVTIKGITINNVPSPN
TDGINPESCRNVHISDCHISVGDDCITIKSGRDAQARRLGVPCENITITNCTMLSGHGGVVIGSEMSGSVRKVTISNCVF
DGTDRGIRIKSTRGRGGVVEDIRVSNIVMSNIKQEAVVLNLKYSQMPAEAKSERTPIFRNVHISGMTVTDVKTPIKIVGL
EEAPISDIVLRDIHIQGARQKCIFEDCERITMDDVIINGEEMKLK

Sequences:

>Translated_445_residues
MNLRITLLAFLCLCATAILRAERVDMLKAGAKANGKALNTKLINSTIDRLNRGGGGTLFFPAGTYLTGSIHLKSNITLEL
EAGATLLFSDNFDDYLPFVEVRHEGVMMKSFQPLIYAVDAENITIKGEGTLDGQGKKWWMEFFRVMIDLKDNGMRDINKY
QPMWDAQNDTTAIYAETNKDYVSTLQRRFFRPPFIQPVRCKKVKIEGVKIVNSPFWTVNPEFCDNVTIKGITINNVPSPN
TDGINPESCRNVHISDCHISVGDDCITIKSGRDAQARRLGVPCENITITNCTMLSGHGGVVIGSEMSGSVRKVTISNCVF
DGTDRGIRIKSTRGRGGVVEDIRVSNIVMSNIKQEAVVLNLKYSQMPAEAKSERTPIFRNVHISGMTVTDVKTPIKIVGL
EEAPISDIVLRDIHIQGARQKCIFEDCERITMDDVIINGEEMKLK
>Mature_445_residues
MNLRITLLAFLCLCATAILRAERVDMLKAGAKANGKALNTKLINSTIDRLNRGGGGTLFFPAGTYLTGSIHLKSNITLEL
EAGATLLFSDNFDDYLPFVEVRHEGVMMKSFQPLIYAVDAENITIKGEGTLDGQGKKWWMEFFRVMIDLKDNGMRDINKY
QPMWDAQNDTTAIYAETNKDYVSTLQRRFFRPPFIQPVRCKKVKIEGVKIVNSPFWTVNPEFCDNVTIKGITINNVPSPN
TDGINPESCRNVHISDCHISVGDDCITIKSGRDAQARRLGVPCENITITNCTMLSGHGGVVIGSEMSGSVRKVTISNCVF
DGTDRGIRIKSTRGRGGVVEDIRVSNIVMSNIKQEAVVLNLKYSQMPAEAKSERTPIFRNVHISGMTVTDVKTPIKIVGL
EEAPISDIVLRDIHIQGARQKCIFEDCERITMDDVIINGEEMKLK

Specific function: Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322613, Length=260, Percent_Identity=25.3846153846154, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 49443; Mature: 49443

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00502 POLYGALACTURONASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRITLLAFLCLCATAILRAERVDMLKAGAKANGKALNTKLINSTIDRLNRGGGGTLFF
CCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCEEEEE
PAGTYLTGSIHLKSNITLELEAGATLLFSDNFDDYLPFVEVRHEGVMMKSFQPLIYAVDA
ECCCEEEEEEEEECEEEEEEECCEEEEEECCCCHHCCEEEECCCCEEEECCCCEEEEEEC
ENITIKGEGTLDGQGKKWWMEFFRVMIDLKDNGMRDINKYQPMWDAQNDTTAIYAETNKD
CEEEEEECCEECCCCHHHHHHHHHEEEEECCCCCCHHHHCCCCCCCCCCCEEEEEECCCH
YVSTLQRRFFRPPFIQPVRCKKVKIEGVKIVNSPFWTVNPEFCDNVTIKGITINNVPSPN
HHHHHHHHHCCCCCCCCCEEEEEEEEEEEEECCCCEEECHHHCCCEEEEEEEECCCCCCC
TDGINPESCRNVHISDCHISVGDDCITIKSGRDAQARRLGVPCENITITNCTMLSGHGGV
CCCCCHHHHCEEEEEEEEEEECCCEEEEECCCCHHHHCCCCCCCCEEEEEEEEEECCCCE
VIGSEMSGSVRKVTISNCVFDGTDRGIRIKSTRGRGGVVEDIRVSNIVMSNIKQEAVVLN
EECCCCCCCEEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEE
LKYSQMPAEAKSERTPIFRNVHISGMTVTDVKTPIKIVGLEEAPISDIVLRDIHIQGARQ
EEHHCCCCCHHHCCCCEEEEEEECCEEEECCCCCEEEEECCCCCCCCEEEEEEEECCCCH
KCIFEDCERITMDDVIINGEEMKLK
HHHHHHHHHCEECCEEECCCEEEEC
>Mature Secondary Structure
MNLRITLLAFLCLCATAILRAERVDMLKAGAKANGKALNTKLINSTIDRLNRGGGGTLFF
CCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCEEEEE
PAGTYLTGSIHLKSNITLELEAGATLLFSDNFDDYLPFVEVRHEGVMMKSFQPLIYAVDA
ECCCEEEEEEEEECEEEEEEECCEEEEEECCCCHHCCEEEECCCCEEEECCCCEEEEEEC
ENITIKGEGTLDGQGKKWWMEFFRVMIDLKDNGMRDINKYQPMWDAQNDTTAIYAETNKD
CEEEEEECCEECCCCHHHHHHHHHEEEEECCCCCCHHHHCCCCCCCCCCCEEEEEECCCH
YVSTLQRRFFRPPFIQPVRCKKVKIEGVKIVNSPFWTVNPEFCDNVTIKGITINNVPSPN
HHHHHHHHHCCCCCCCCCEEEEEEEEEEEEECCCCEEECHHHCCCEEEEEEEECCCCCCC
TDGINPESCRNVHISDCHISVGDDCITIKSGRDAQARRLGVPCENITITNCTMLSGHGGV
CCCCCHHHHCEEEEEEEEEEECCCEEEEECCCCHHHHCCCCCCCCEEEEEEEEEECCCCE
VIGSEMSGSVRKVTISNCVFDGTDRGIRIKSTRGRGGVVEDIRVSNIVMSNIKQEAVVLN
EECCCCCCCEEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEE
LKYSQMPAEAKSERTPIFRNVHISGMTVTDVKTPIKIVGLEEAPISDIVLRDIHIQGARQ
EEHHCCCCCHHHCCCCEEEEEEECCEEEECCCCCEEEEECCCCCCCCEEEEEEEECCCCH
KCIFEDCERITMDDVIINGEEMKLK
HHHHHHHHHCEECCEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1860822 [H]