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The map label for this gene is srlE [H]

Identifier: 29143093

GI number: 29143093

Start: 2817613

End: 2818584

Strand: Direct

Name: srlE [H]

Synonym: t2734

Alternate gene names: 29143093

Gene position: 2817613-2818584 (Clockwise)

Preceding gene: 29143092

Following gene: 29143094

Centisome position: 58.8

GC content: 57.72

Gene sequence:

>972_bases
ATGACACGGGTTCGCATTGAGAAAGGTGCCGGCGGCTGGGGCGGCCCGCTTGAACTGGACGTTACGCCAGGCAAAAAGAT
CGTCTATATCACAGCCGGTACGCGCCCGGCGATCGTCGACAAACTGGCGCAACTAACAGGCTGGCAAGCGGTGGACGGCT
TTAAAGAAGGCGAACCGCCGGAAGCGGAGATCGGCGCGGCCATTATCGACTGCGGCGGTACGCTGCGCTGCGGTATCTAC
CCGAAACGCCGGATTCCAACCATTAATATTCACTCGACGGGTAAGTCCGGCCCACTGGCGCAGTATATTGTTGAAGATAT
TTATGTCTCCGGCGTAAAAGAAGAAAACATTACTCTAGTCGGCGAAACATCTGCCAGACCCCAGCCTGCCAAAACGACAT
TAGGACGTGACTACGACACCAGCAAAAAAATCACCGAGCAGAGCGACGGGCTGCTGGCAAAAGTCGGTATGGGAATGGGT
TCTGCCGTGGCGGTACTGTTCCAGTCCGGTCGCGACACCATTGATACGGTCCTGAAAACAATTCTGCCGTTTATGGCGTT
CGTTTCGGCGCTAATCGGCATCATTATGGCCTCAGGTCTTGGCGACTGGATCGCCCACGGCCTGGCGCCATTAGCCAGCC
ATCCACTGGGGCTGGTGACGCTGGCATTGATCTGCTCTTTCCCGCTGCTGTCGCCCTTTCTCGGCCCAGGCGCGGTTATC
GCTCAGGTTATTGGCGTTCTGATCGGCGTTCAGATAGGTCTGGGCAATATCCCCCCGCATCTGGCGCTGCCCGCCCTGTT
CGCGATTAACGCGCAGGCGGCCTGCGACTTTATCCCGGTCGGCCTGTCACTGGCGGAAGCGAAACAAGACACCGTTCGCG
TCGGCGTACCTTCTGTGCTGGTCGGACGCTTCCTGACCGGCGCGCCCACGGTACTTATCGCCTGGTTTGTTTCCGGCTTT
ATCTATCAATAA

Upstream 100 bases:

>100_bases
ACTTCTTCCGCGGTTGGGTCACCGATCTCACCACCGCTATTTTTGAGAAAAAAATGGGGATTCAACTTGAGCAGAAAGTT
CATCTGACAGGAGCGGTATC

Downstream 100 bases:

>100_bases
GAGGTTCAGACATGAGCGTGATTTATCAAACCACCATCACCCGCATCGGCCAGAGCGCGATGGAGGCGCTCGGCGAAAAG
ATGCTGATTACGTTTCGGGA

Product: PTS system glucitol/sorbitol-specific transporter subunit IIBC

Products: NA

Alternate protein names: EII-Gut; PTS system glucitol/sorbitol-specific EIIB component [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTRVRIEKGAGGWGGPLELDVTPGKKIVYITAGTRPAIVDKLAQLTGWQAVDGFKEGEPPEAEIGAAIIDCGGTLRCGIY
PKRRIPTINIHSTGKSGPLAQYIVEDIYVSGVKEENITLVGETSARPQPAKTTLGRDYDTSKKITEQSDGLLAKVGMGMG
SAVAVLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVTLALICSFPLLSPFLGPGAVI
AQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEAKQDTVRVGVPSVLVGRFLTGAPTVLIAWFVSGF
IYQ

Sequences:

>Translated_323_residues
MTRVRIEKGAGGWGGPLELDVTPGKKIVYITAGTRPAIVDKLAQLTGWQAVDGFKEGEPPEAEIGAAIIDCGGTLRCGIY
PKRRIPTINIHSTGKSGPLAQYIVEDIYVSGVKEENITLVGETSARPQPAKTTLGRDYDTSKKITEQSDGLLAKVGMGMG
SAVAVLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVTLALICSFPLLSPFLGPGAVI
AQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEAKQDTVRVGVPSVLVGRFLTGAPTVLIAWFVSGF
IYQ
>Mature_322_residues
TRVRIEKGAGGWGGPLELDVTPGKKIVYITAGTRPAIVDKLAQLTGWQAVDGFKEGEPPEAEIGAAIIDCGGTLRCGIYP
KRRIPTINIHSTGKSGPLAQYIVEDIYVSGVKEENITLVGETSARPQPAKTTLGRDYDTSKKITEQSDGLLAKVGMGMGS
AVAVLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVTLALICSFPLLSPFLGPGAVIA
QVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEAKQDTVRVGVPSVLVGRFLTGAPTVLIAWFVSGFI
YQ

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3732

COG function: function code G; Phosphotransferase system sorbitol-specific component IIBC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI48994905, Length=323, Percent_Identity=92.8792569659443, Blast_Score=596, Evalue=1e-172,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011638
- InterPro:   IPR011618
- InterPro:   IPR004702 [H]

Pfam domain/function: PF07663 EIIBC-GUT_C; PF03612 EIIBC-GUT_N [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 33715; Mature: 33584

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00107 PROTEIN_KINASE_ATP ; PS51102 PTS_EIIB_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVRIEKGAGGWGGPLELDVTPGKKIVYITAGTRPAIVDKLAQLTGWQAVDGFKEGEPP
CCEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHCCCHHHCCCCCCCCC
EAEIGAAIIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQYIVEDIYVSGVKEENITLV
CHHHCEEEEECCCEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEE
GETSARPQPAKTTLGRDYDTSKKITEQSDGLLAKVGMGMGSAVAVLFQSGRDTIDTVLKT
ECCCCCCCCCHHHCCCCCCCCHHHHHHCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHH
ILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVTLALICSFPLLSPFLGPGAVI
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHH
AQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEAKQDTVRVGVPSVL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHCCEEECCCHHHHHCCCCEECCCHHHH
VGRFLTGAPTVLIAWFVSGFIYQ
HHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRVRIEKGAGGWGGPLELDVTPGKKIVYITAGTRPAIVDKLAQLTGWQAVDGFKEGEPP
CEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHCCCHHHCCCCCCCCC
EAEIGAAIIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQYIVEDIYVSGVKEENITLV
CHHHCEEEEECCCEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEE
GETSARPQPAKTTLGRDYDTSKKITEQSDGLLAKVGMGMGSAVAVLFQSGRDTIDTVLKT
ECCCCCCCCCHHHCCCCCCCCHHHHHHCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHH
ILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVTLALICSFPLLSPFLGPGAVI
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHH
AQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPVGLSLAEAKQDTVRVGVPSVL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHCCEEECCCHHHHHCCCCEECCCHHHH
VGRFLTGAPTVLIAWFVSGFIYQ
HHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3553176; 9205837; 9278503 [H]