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The map label for this gene is srlA [H]

Identifier: 29143092

GI number: 29143092

Start: 2817053

End: 2817616

Strand: Direct

Name: srlA [H]

Synonym: t2733

Alternate gene names: 29143092

Gene position: 2817053-2817616 (Clockwise)

Preceding gene: 29143076

Following gene: 29143093

Centisome position: 58.79

GC content: 51.24

Gene sequence:

>564_bases
ATGATCGAAACCATTACACATGGTGCGGAGTGGTTCATCGGGTTATTCCAGAAAGGCGGTGAGGTGTTTACCGGTATGGT
GACAGGTATTCTTCCGCTACTGATAAGCCTGCTGGTTATCATGAATGCGCTCATCAATTTTATCGGCCAGCAGCGTATTG
AGCGTTTTGCCCAACGCTGCGCCGGAAATCCGCTATCCCGCTACCTGCTTTTACCGTTTATCGGGACGTTTGTTTTTTGT
AATCCGATGACCCTGAGTCTGGGACGCTTTATGCCGGAGAAGTACAAGCCGAGCTATTACGCGGCGGCCTCCTATAGCTG
TCACTCCATGAACGGTCTGTTTCCGCATATCAATCCCGGCGAACTGTTTGTTTATCTGGGTATCGCCAGCGGACTCACCA
CGTTAGGGCTTCCTCTCGGCCCGCTCGCGGTGAGCTATCTGCTGGTAGGTCTGGTGACGAACTTCTTCCGCGGTTGGGTC
ACCGATCTCACCACCGCTATTTTTGAGAAAAAAATGGGGATTCAACTTGAGCAGAAAGTTCATCTGACAGGAGCGGTATC
ATGA

Upstream 100 bases:

>100_bases
TCTTTTATTTTGAGATCAAAATAACACTTTTATACCTTTCAACATGATTACAATGAGCAGGAAGTTAGCAAGAAAACTAA
AAACCCTACAGGAGAGAACA

Downstream 100 bases:

>100_bases
CACGGGTTCGCATTGAGAAAGGTGCCGGCGGCTGGGGCGGCCCGCTTGAACTGGACGTTACGCCAGGCAAAAAGATCGTC
TATATCACAGCCGGTACGCG

Product: PTS system glucitol/sorbitol-specific transporter subunit IIBC

Products: protein histidine; sugar phosphate

Alternate protein names: EIIC-Gut; PTS system glucitol/sorbitol-specific EIIC component [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNALINFIGQQRIERFAQRCAGNPLSRYLLLPFIGTFVFC
NPMTLSLGRFMPEKYKPSYYAAASYSCHSMNGLFPHINPGELFVYLGIASGLTTLGLPLGPLAVSYLLVGLVTNFFRGWV
TDLTTAIFEKKMGIQLEQKVHLTGAVS

Sequences:

>Translated_187_residues
MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNALINFIGQQRIERFAQRCAGNPLSRYLLLPFIGTFVFC
NPMTLSLGRFMPEKYKPSYYAAASYSCHSMNGLFPHINPGELFVYLGIASGLTTLGLPLGPLAVSYLLVGLVTNFFRGWV
TDLTTAIFEKKMGIQLEQKVHLTGAVS
>Mature_187_residues
MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNALINFIGQQRIERFAQRCAGNPLSRYLLLPFIGTFVFC
NPMTLSLGRFMPEKYKPSYYAAASYSCHSMNGLFPHINPGELFVYLGIASGLTTLGLPLGPLAVSYLLVGLVTNFFRGWV
TDLTTAIFEKKMGIQLEQKVHLTGAVS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3730

COG function: function code G; Phosphotransferase system sorbitol-specific component IIC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI48994904, Length=187, Percent_Identity=96.7914438502674, Blast_Score=368, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004699 [H]

Pfam domain/function: PF03608 EII-GUT [H]

EC number: 2.7.1.69

Molecular weight: Translated: 20601; Mature: 20601

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS51107 PTS_EIIC_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNALINFIGQQRIERFAQRC
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGNPLSRYLLLPFIGTFVFCNPMTLSLGRFMPEKYKPSYYAAASYSCHSMNGLFPHINPG
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHCCCCEEEECCCCHHCCCCCCCCCCCC
ELFVYLGIASGLTTLGLPLGPLAVSYLLVGLVTNFFRGWVTDLTTAIFEKKMGIQLEQKV
CEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHE
HLTGAVS
EECCCCC
>Mature Secondary Structure
MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNALINFIGQQRIERFAQRC
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGNPLSRYLLLPFIGTFVFCNPMTLSLGRFMPEKYKPSYYAAASYSCHSMNGLFPHINPG
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHCCCCEEEECCCCHHCCCCCCCCCCCC
ELFVYLGIASGLTTLGLPLGPLAVSYLLVGLVTNFFRGWVTDLTTAIFEKKMGIQLEQKV
CEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHE
HLTGAVS
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3553176; 9205837; 9278503; 1334233 [H]