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The map label for this gene is emrB [H]

Identifier: 29143076

GI number: 29143076

Start: 2801719

End: 2803257

Strand: Direct

Name: emrB [H]

Synonym: t2712

Alternate gene names: 29143076

Gene position: 2801719-2803257 (Clockwise)

Preceding gene: 29143075

Following gene: 29143092

Centisome position: 58.47

GC content: 56.99

Gene sequence:

>1539_bases
ATGCAACAGCAAAAACCGCTGGAAGGCGCGCAGCTCATCATCATGACGATCGCGCTGTCGCTGGCGACATTCATGCAGGT
GCTGGACTCCACCATTGCCAACGTGGCGATTCCGACCATCGCCGGGAACCTGGGTTCTTCCCTAAGCCAGGGGACATGGG
TTATCACCTCTTTCGGGGTGGCGAACGCTATCTCGATTCCCTTAACCGGCTGGCTGGCGAAGCGTTTTGGCGAAGTCAAA
CTGTTCATGTGGTCGACGGTCGCCTTTGCCGCGGCCTCCTGGGCCTGTGGCGTATCCAGCAGCCTGAACATGCTGATCTT
CTTCCGCGTGGTGCAGGGCGTGGTCGCCGGGCCGCTGATTCCGCTCTCGCAAAGCCTGTTGCTGAATAACTATCCGCCTG
CAAAGCGCTCCATTGCGCTGGCGCTCTGGTCAATGACCGTCATTGTCGCGCCGATTTGCGGCCCGATTCTGGGGGGCTAC
ATCAGTGATAATTACCACTGGGGATGGATTTTCTTCATCAACGTGCCGATTGGCATCGCCGTCGTCCTGATGACGTTACA
CACGCTGCGCGGACGTGAAACTCATACCGAACGGCGACGTATTGACGCCGTCGGGCTGGCGTTACTGGTGATTGGCATCA
GCAGCCTGCAAATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTCTCCTCGCAGGAAATTATCATCCTGACCGTGGTC
GCCGTCATCGCCATCAGTTTCCTGATCGTCTGGGAACTGACCGACGATCACCCTATCGTCGATCTTTCTCTTTTTAAATC
GCGTAACTTCACTATTGGCTGCCTGTGCATCAGCCTTGCCTATATGCTCTATTTCGGCGCCATTGTTCTATTGCCGCAGC
TTTTGCAAGAGGTGTACGGCTATACCGCAACGTGGGCAGGTCTGGCGTCGGCGCCAGTCGGTATTATTCCGGTAATTTTG
TCGCCGATTATCGGACGCTTCGCGCATAAACTGGATATGCGCAGGCTGGTCACATTCAGCTTCATTATGTACGCCGTCTG
TTTTTACTGGCGCGCCTGGACGTTCGAACCGGGTATGGATTTCGGCGCTTCCGCATGGCCGCAGTTTATTCAAGGATTTG
CCGTCGCCTGCTTCTTTATGCCGCTGACCACCATTACGCTCTCCGGGTTACCGCCCGAACGGCTGGCGGCGGCATCAAGC
CTGTCGAACTTCACGCGAACGCTGGCGGGCTCCATTGGCACCTCGATTACCACCACTATGTGGACCGATCGTGAGTCGCT
ACACCATGCGCAGCTCACCGAGTCGGTTACGGCGTATAATCCAAACGCTCAGGCTATGTACGATAAACTGGAAGGCTTAG
GGATGACGCACCAACAGGCGTCAGGCTGGATAGCCCAGCAAATCACGAATCAGGGGCTGATTATTTCCGCCAACGAAATC
TTCTGGATGTCGGCGGGGATCTTCCTGGTGCTGCTCGGCCTGGTGTGGTTCGCCAAACCGCCCTTCGGTGCGGGCGGCGG
CGGCGGTGGCGCGCACTAA

Upstream 100 bases:

>100_bases
GTTGCCGAAAGTAACGCACGCGAAATTAATCTCGCGCCGGTCAATAAACTGATCGACGACATCGTACAGGCTAACGCGGG
TTAATCCTGAGGTGCGTGTG

Downstream 100 bases:

>100_bases
CTATCTGATAAAAGGGATCTACGGATCCCTTTTTTAGTAGACCAACAATCAGAAGAAATATTTGAATCTCAGACGCCCGC
CGTAACGCGCATCTTCACTG

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MQQQKPLEGAQLIIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRFGEVK
LFMWSTVAFAAASWACGVSSSLNMLIFFRVVQGVVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGIAVVLMTLHTLRGRETHTERRRIDAVGLALLVIGISSLQIMLDRGKELDWFSSQEIIILTVV
AVIAISFLIVWELTDDHPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVIL
SPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASS
LSNFTRTLAGSIGTSITTTMWTDRESLHHAQLTESVTAYNPNAQAMYDKLEGLGMTHQQASGWIAQQITNQGLIISANEI
FWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH

Sequences:

>Translated_512_residues
MQQQKPLEGAQLIIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRFGEVK
LFMWSTVAFAAASWACGVSSSLNMLIFFRVVQGVVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGIAVVLMTLHTLRGRETHTERRRIDAVGLALLVIGISSLQIMLDRGKELDWFSSQEIIILTVV
AVIAISFLIVWELTDDHPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVIL
SPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASS
LSNFTRTLAGSIGTSITTTMWTDRESLHHAQLTESVTAYNPNAQAMYDKLEGLGMTHQQASGWIAQQITNQGLIISANEI
FWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH
>Mature_512_residues
MQQQKPLEGAQLIIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRFGEVK
LFMWSTVAFAAASWACGVSSSLNMLIFFRVVQGVVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGIAVVLMTLHTLRGRETHTERRRIDAVGLALLVIGISSLQIMLDRGKELDWFSSQEIIILTVV
AVIAISFLIVWELTDDHPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVIL
SPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASS
LSNFTRTLAGSIGTSITTTMWTDRESLHHAQLTESVTAYNPNAQAMYDKLEGLGMTHQQASGWIAQQITNQGLIISANEI
FWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=502, Percent_Identity=95.4183266932271, Blast_Score=944, Evalue=0.0,
Organism=Escherichia coli, GI1788710, Length=506, Percent_Identity=62.4505928853755, Blast_Score=629, Evalue=0.0,
Organism=Escherichia coli, GI1790195, Length=423, Percent_Identity=32.387706855792, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87081983, Length=421, Percent_Identity=27.790973871734, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=29.4554455445545, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322958, Length=534, Percent_Identity=23.2209737827715, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319770, Length=313, Percent_Identity=25.8785942492013, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=29.3333333333333, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55696; Mature: 55696

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQQKPLEGAQLIIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEECCC
ANAISIPLTGWLAKRFGEVKLFMWSTVAFAAASWACGVSSSLNMLIFFRVVQGVVAGPLI
HHEEECCHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
PLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPIGIA
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECHHHHH
VVLMTLHTLRGRETHTERRRIDAVGLALLVIGISSLQIMLDRGKELDWFSSQEIIILTVV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AVIAISFLIVWELTDDHPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYG
HHHHHHHHHEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YTATWAGLASAPVGIIPVILSPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMD
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASSLSNFTRTLAGSIGTSITTTM
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
WTDRESLHHAQLTESVTAYNPNAQAMYDKLEGLGMTHQQASGWIAQQITNQGLIISANEI
ECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCEEEECCCE
FWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH
EEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQQKPLEGAQLIIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEECCC
ANAISIPLTGWLAKRFGEVKLFMWSTVAFAAASWACGVSSSLNMLIFFRVVQGVVAGPLI
HHEEECCHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
PLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPIGIA
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECHHHHH
VVLMTLHTLRGRETHTERRRIDAVGLALLVIGISSLQIMLDRGKELDWFSSQEIIILTVV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AVIAISFLIVWELTDDHPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYG
HHHHHHHHHEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YTATWAGLASAPVGIIPVILSPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMD
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASSLSNFTRTLAGSIGTSITTTM
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
WTDRESLHHAQLTESVTAYNPNAQAMYDKLEGLGMTHQQASGWIAQQITNQGLIISANEI
ECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCEEEECCCE
FWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH
EEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]