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The map label for this gene is dpiB [H]

Identifier: 29142642

GI number: 29142642

Start: 2306619

End: 2308280

Strand: Reverse

Name: dpiB [H]

Synonym: t2242

Alternate gene names: 29142642

Gene position: 2308280-2306619 (Counterclockwise)

Preceding gene: 29142652

Following gene: 29142641

Centisome position: 48.17

GC content: 51.87

Gene sequence:

>1662_bases
ATGTCCTATCAGAAAAATAAAAAGCTGTTTCCTTTTTTTCGGCAACTCGCGTTTCCGTTACGTATTTTTCTGCTCATTCT
GGTGGTTTCTGTTTTCATCGTGGCAGCGTTAGCACAATATTTGTCTGCCAGTTTCGAAGATTATCTGGCGTCCCATGTTC
GTGACATGGCGATGAATCAGGCAAAAATTATTGCTTCTAACGACAGCATTATCGCTGCCGTAAAAAACCGGGACTATAAA
CGCCTGGCGATAATTGCGAATAAACTGCAGCGCGGGACGGATTTTGACTATGTGGTGATAGGCGATCGCCACTCGATCCG
GCTTTACCATCCGAATCCGGAAAAAATCGGCTATCCCATGCAGTTTACGAAACCCGGCGCGCTGGAAAGAGGAGAAAGTT
ATTTCATTACCGGTAAGGGCTCAATAGGGATGGCGATGCGCGCCAAAACGCCTATCTTTGACAACGAAGGCAACGTGATC
GGCGTTGTTTCAATTGGCTATCTGGTCAGTAAAATCGACTCCTGGCGGCTGGACTTCTTATTACCGATGGCGGGGGTTTT
TGTCCTGTTGCTGGTGGTATTAATGCTGCTTTCCTGGTTTTTCGCCGCGCATATCCGCCGACAAATGCTGGGCATGGAGC
CGAAACAGATTGCGCGCGTGGTACGCCAGCAGGAGGCGTTATTTAGCTCGGTTTATGAAGGGCTGATCGCCGTTGATCCG
GAGGGACATATCACCGCGATTAACCGCAATGCGAGAAAAATGTTGGGGCTGCCGTCGCCGGGGCGTCAGTGGCTGGGCAA
GCTGATAGCGGAAGTCGTCAATCCGGCAGACTTTTTTACCTGCCAGATCGCAGAAAGACGCCAGGACGTGATGGCCAACT
TCAATGGCCTGAGCGTCATCGCCAATCGTGAGGCTATCCGTTCCGGCGAGGAGCTGCTGGGCGCTATTATTAGTTTTCGC
AGCAAAGATGAAATCGCGACGTTGAACGCGCAGCTAACCCAGATTAAACAGTATGTTGAGAGCCTGCGCACGCTACGCCA
TGAGCACCTGAACTGGATGTCGACTCTGAACGGCCTGCTGCAAATGAAAGAGTATGATCGGGTCAGAGAGATGGTGCAGG
GCGAATCGCAGGCGCAGCAGCAACTTATTGATAGTTTACGCGGCGCCTTCGCCGATCGTCAGGTGGCCGGTTTGCTGTTT
GGCAAAGTTCAGCGCGCGCGTGAACTGGGGCTCAAGATGGTGATTGTTCCCGGCAGCCAGTTACATCAACTGCCGGAGGG
GCTGGATAGCACGGAGTTCGCCGCCATCGTCGGGAATCTGCTGGATAACGCGTTTGAGGCGAGTTTACGTACACAGGAAG
GCGACAATACCATCGAACTTTTCCTGAGCGACGAGGGCGACGAGGTGGTGATAGAGGTCGCCGACCAGGGCTGCGGCGTT
CCTGAAGCGCTGCGGGAAAAAATTTTTGAGCAGGGCGTAAGTACCCGAGCTGACGAACCCGGTGAGCATGGTATCGGGTT
ATATCTGATTGCAAGTTATGTGGGGCGCTGTGGCGGGGTCATTACGCTGGAAGACAATGATCCTTGTGGCACCTTATTTT
CTTTATTCCTTCCGAAAGTGAAAAAAAACGATGACAGAACCATTAACCCTATTGATCGTTGA

Upstream 100 bases:

>100_bases
GGGAATCTGTAACGATTGGTTTTTATATGCTTCAAGATACTCAAAACATTTTTATTACATTTGCGATAAATCTGTAGAGA
AATACCCATAAGGAACCTTA

Downstream 100 bases:

>100_bases
GGATGAAACGCTGCTGGCGGAAATGCACGCAGAATATATCCGCCATATTCCTGGCTTTAACCAGATATGGCTGGCGGGCA
ATCTGGCGCAGGCGAGAATG

Product: sensor kinase DpiB

Products: NA

Alternate protein names: Sensor histidine kinase CitA [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MSYQKNKKLFPFFRQLAFPLRIFLLILVVSVFIVAALAQYLSASFEDYLASHVRDMAMNQAKIIASNDSIIAAVKNRDYK
RLAIIANKLQRGTDFDYVVIGDRHSIRLYHPNPEKIGYPMQFTKPGALERGESYFITGKGSIGMAMRAKTPIFDNEGNVI
GVVSIGYLVSKIDSWRLDFLLPMAGVFVLLLVVLMLLSWFFAAHIRRQMLGMEPKQIARVVRQQEALFSSVYEGLIAVDP
EGHITAINRNARKMLGLPSPGRQWLGKLIAEVVNPADFFTCQIAERRQDVMANFNGLSVIANREAIRSGEELLGAIISFR
SKDEIATLNAQLTQIKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVREMVQGESQAQQQLIDSLRGAFADRQVAGLLF
GKVQRARELGLKMVIVPGSQLHQLPEGLDSTEFAAIVGNLLDNAFEASLRTQEGDNTIELFLSDEGDEVVIEVADQGCGV
PEALREKIFEQGVSTRADEPGEHGIGLYLIASYVGRCGGVITLEDNDPCGTLFSLFLPKVKKNDDRTINPIDR

Sequences:

>Translated_553_residues
MSYQKNKKLFPFFRQLAFPLRIFLLILVVSVFIVAALAQYLSASFEDYLASHVRDMAMNQAKIIASNDSIIAAVKNRDYK
RLAIIANKLQRGTDFDYVVIGDRHSIRLYHPNPEKIGYPMQFTKPGALERGESYFITGKGSIGMAMRAKTPIFDNEGNVI
GVVSIGYLVSKIDSWRLDFLLPMAGVFVLLLVVLMLLSWFFAAHIRRQMLGMEPKQIARVVRQQEALFSSVYEGLIAVDP
EGHITAINRNARKMLGLPSPGRQWLGKLIAEVVNPADFFTCQIAERRQDVMANFNGLSVIANREAIRSGEELLGAIISFR
SKDEIATLNAQLTQIKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVREMVQGESQAQQQLIDSLRGAFADRQVAGLLF
GKVQRARELGLKMVIVPGSQLHQLPEGLDSTEFAAIVGNLLDNAFEASLRTQEGDNTIELFLSDEGDEVVIEVADQGCGV
PEALREKIFEQGVSTRADEPGEHGIGLYLIASYVGRCGGVITLEDNDPCGTLFSLFLPKVKKNDDRTINPIDR
>Mature_552_residues
SYQKNKKLFPFFRQLAFPLRIFLLILVVSVFIVAALAQYLSASFEDYLASHVRDMAMNQAKIIASNDSIIAAVKNRDYKR
LAIIANKLQRGTDFDYVVIGDRHSIRLYHPNPEKIGYPMQFTKPGALERGESYFITGKGSIGMAMRAKTPIFDNEGNVIG
VVSIGYLVSKIDSWRLDFLLPMAGVFVLLLVVLMLLSWFFAAHIRRQMLGMEPKQIARVVRQQEALFSSVYEGLIAVDPE
GHITAINRNARKMLGLPSPGRQWLGKLIAEVVNPADFFTCQIAERRQDVMANFNGLSVIANREAIRSGEELLGAIISFRS
KDEIATLNAQLTQIKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVREMVQGESQAQQQLIDSLRGAFADRQVAGLLFG
KVQRARELGLKMVIVPGSQLHQLPEGLDSTEFAAIVGNLLDNAFEASLRTQEGDNTIELFLSDEGDEVVIEVADQGCGVP
EALREKIFEQGVSTRADEPGEHGIGLYLIASYVGRCGGVITLEDNDPCGTLFSLFLPKVKKNDDRTINPIDR

Specific function: Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox c

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=546, Percent_Identity=87.9120879120879, Blast_Score=992, Evalue=0.0,
Organism=Escherichia coli, GI1790567, Length=544, Percent_Identity=25.9191176470588, Blast_Score=182, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61814; Mature: 61683

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQKNKKLFPFFRQLAFPLRIFLLILVVSVFIVAALAQYLSASFEDYLASHVRDMAMNQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AKIIASNDSIIAAVKNRDYKRLAIIANKLQRGTDFDYVVIGDRHSIRLYHPNPEKIGYPM
CEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCHHHCCCCC
QFTKPGALERGESYFITGKGSIGMAMRAKTPIFDNEGNVIGVVSIGYLVSKIDSWRLDFL
CCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCEEEEEHHHHHHHHHCCHHHHHH
LPMAGVFVLLLVVLMLLSWFFAAHIRRQMLGMEPKQIARVVRQQEALFSSVYEGLIAVDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECC
EGHITAINRNARKMLGLPSPGRQWLGKLIAEVVNPADFFTCQIAERRQDVMANFNGLSVI
CCCEEEECCCHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEE
ANREAIRSGEELLGAIISFRSKDEIATLNAQLTQIKQYVESLRTLRHEHLNWMSTLNGLL
ECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QMKEYDRVREMVQGESQAQQQLIDSLRGAFADRQVAGLLFGKVQRARELGLKMVIVPGSQ
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHH
LHQLPEGLDSTEFAAIVGNLLDNAFEASLRTQEGDNTIELFLSDEGDEVVIEVADQGCGV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCC
PEALREKIFEQGVSTRADEPGEHGIGLYLIASYVGRCGGVITLEDNDPCGTLFSLFLPKV
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCHH
KKNDDRTINPIDR
CCCCCCCCCCCCC
>Mature Secondary Structure 
SYQKNKKLFPFFRQLAFPLRIFLLILVVSVFIVAALAQYLSASFEDYLASHVRDMAMNQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AKIIASNDSIIAAVKNRDYKRLAIIANKLQRGTDFDYVVIGDRHSIRLYHPNPEKIGYPM
CEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCHHHCCCCC
QFTKPGALERGESYFITGKGSIGMAMRAKTPIFDNEGNVIGVVSIGYLVSKIDSWRLDFL
CCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCEEEEEHHHHHHHHHCCHHHHHH
LPMAGVFVLLLVVLMLLSWFFAAHIRRQMLGMEPKQIARVVRQQEALFSSVYEGLIAVDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECC
EGHITAINRNARKMLGLPSPGRQWLGKLIAEVVNPADFFTCQIAERRQDVMANFNGLSVI
CCCEEEECCCHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEE
ANREAIRSGEELLGAIISFRSKDEIATLNAQLTQIKQYVESLRTLRHEHLNWMSTLNGLL
ECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QMKEYDRVREMVQGESQAQQQLIDSLRGAFADRQVAGLLFGKVQRARELGLKMVIVPGSQ
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHH
LHQLPEGLDSTEFAAIVGNLLDNAFEASLRTQEGDNTIELFLSDEGDEVVIEVADQGCGV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCC
PEALREKIFEQGVSTRADEPGEHGIGLYLIASYVGRCGGVITLEDNDPCGTLFSLFLPKV
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCHH
KKNDDRTINPIDR
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9701802; 8905232; 9278503 [H]