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The map label for this gene is dpiA [H]

Identifier: 29142641

GI number: 29142641

Start: 2305970

End: 2306650

Strand: Reverse

Name: dpiA [H]

Synonym: t2241

Alternate gene names: 29142641

Gene position: 2306650-2305970 (Counterclockwise)

Preceding gene: 29142642

Following gene: 29142639

Centisome position: 48.14

GC content: 53.3

Gene sequence:

>681_bases
ATGACAGAACCATTAACCCTATTGATCGTTGAGGATGAAACGCTGCTGGCGGAAATGCACGCAGAATATATCCGCCATAT
TCCTGGCTTTAACCAGATATGGCTGGCGGGCAATCTGGCGCAGGCGAGAATGATGATTGACCGGTTCAAACCTGGATTAA
TTCTGTTGGATAATTATCTGCCGGATGGGAAGGGGATTACGCTGCTGCATGAATTAATGCAGTCGCGCTATCCCGGCGGC
GTCGTGTTTACGACCGCCGCCAGTGACATGGAGACGGTAGCGGAAGCGGTACGTAGCGGCGCGTTTGATTATCTGGTCAA
ACCTATTGCTTATGAGCGTTTGGGGCAGACGCTGACCCGTTATCAACAGCGCAGACGTATGCTGGCGAGCGCGGATAGCG
CAAGCCAGAAGCAGATAGATGAAATGTTCAACGCCTATGCGCGCGGCGAACCGAAAGGGGATTTGCCGACAGGTATTGAT
GCCTTAACCCTTAATGCGGTGATGAAGCTGTTTGCCGATCCAACCGTACGCCATACGGCTGAAACGGTCGCGCAGGCCTT
AACGATAAGCCGCACCACATCCCGACGCTACCTGGAATATTGCGCGAGTCGGCACCTGATTGTCGCAGAGATTATTCATG
GCAAAGTGGGCAGGCCGCAGCGTATTTATCACGGCGGCTAA

Upstream 100 bases:

>100_bases
GCAAGTTATGTGGGGCGCTGTGGCGGGGTCATTACGCTGGAAGACAATGATCCTTGTGGCACCTTATTTTCTTTATTCCT
TCCGAAAGTGAAAAAAAACG

Downstream 100 bases:

>100_bases
GCCCGCTTTAAGATGCCGGAGCGATGTTTTCCCTCGCCCCGGCTTACCGGCTATCAGCCGCCGGTGACGGCGGAAGAGGC
GCCTGGCACCATAATTTCCG

Product: two-component response regulator DpiA

Products: NA

Alternate protein names: Destabilizer of plasmid inheritance [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MTEPLTLLIVEDETLLAEMHAEYIRHIPGFNQIWLAGNLAQARMMIDRFKPGLILLDNYLPDGKGITLLHELMQSRYPGG
VVFTTAASDMETVAEAVRSGAFDYLVKPIAYERLGQTLTRYQQRRRMLASADSASQKQIDEMFNAYARGEPKGDLPTGID
ALTLNAVMKLFADPTVRHTAETVAQALTISRTTSRRYLEYCASRHLIVAEIIHGKVGRPQRIYHGG

Sequences:

>Translated_226_residues
MTEPLTLLIVEDETLLAEMHAEYIRHIPGFNQIWLAGNLAQARMMIDRFKPGLILLDNYLPDGKGITLLHELMQSRYPGG
VVFTTAASDMETVAEAVRSGAFDYLVKPIAYERLGQTLTRYQQRRRMLASADSASQKQIDEMFNAYARGEPKGDLPTGID
ALTLNAVMKLFADPTVRHTAETVAQALTISRTTSRRYLEYCASRHLIVAEIIHGKVGRPQRIYHGG
>Mature_225_residues
TEPLTLLIVEDETLLAEMHAEYIRHIPGFNQIWLAGNLAQARMMIDRFKPGLILLDNYLPDGKGITLLHELMQSRYPGGV
VFTTAASDMETVAEAVRSGAFDYLVKPIAYERLGQTLTRYQQRRRMLASADSASQKQIDEMFNAYARGEPKGDLPTGIDA
LTLNAVMKLFADPTVRHTAETVAQALTISRTTSRRYLEYCASRHLIVAEIIHGKVGRPQRIYHGG

Specific function: Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox c

COG id: COG4565

COG function: function code KT; Response regulator of citrate/malate metabolism

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786838, Length=226, Percent_Identity=85.3982300884956, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1790566, Length=220, Percent_Identity=29.0909090909091, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25307; Mature: 25175

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPLTLLIVEDETLLAEMHAEYIRHIPGFNQIWLAGNLAQARMMIDRFKPGLILLDNYL
CCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCEEEEECCC
PDGKGITLLHELMQSRYPGGVVFTTAASDMETVAEAVRSGAFDYLVKPIAYERLGQTLTR
CCCCCHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YQQRRRMLASADSASQKQIDEMFNAYARGEPKGDLPTGIDALTLNAVMKLFADPTVRHTA
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
ETVAQALTISRTTSRRYLEYCASRHLIVAEIIHGKVGRPQRIYHGG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TEPLTLLIVEDETLLAEMHAEYIRHIPGFNQIWLAGNLAQARMMIDRFKPGLILLDNYL
CCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCEEEEECCC
PDGKGITLLHELMQSRYPGGVVFTTAASDMETVAEAVRSGAFDYLVKPIAYERLGQTLTR
CCCCCHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YQQRRRMLASADSASQKQIDEMFNAYARGEPKGDLPTGIDALTLNAVMKLFADPTVRHTA
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
ETVAQALTISRTTSRRYLEYCASRHLIVAEIIHGKVGRPQRIYHGG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]