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The map label for this gene is poxB [H]

Identifier: 29142419

GI number: 29142419

Start: 2057552

End: 2059270

Strand: Direct

Name: poxB [H]

Synonym: t1998

Alternate gene names: 29142419

Gene position: 2057552-2059270 (Clockwise)

Preceding gene: 29142418

Following gene: 29142420

Centisome position: 42.94

GC content: 57.07

Gene sequence:

>1719_bases
ATGAAACAAACCGTTGCTGCTTTTATCGCCAAAACGCTGGAACAGGCTGGCGTAAAACAAATCTGGGGCGTCACTGGCGA
CTCCCTGAACGGCCTGAGCGATAGTCTTAATCGTATGGGAACCATTGAATGGATGCCAACCCGCCACGAAGAGGTCGCCG
CTTTCGCCGCCGGCGCACAAGCGCAGCTTACCGGCGAGCTGGCAGTGTGCGCCGGTTCATGTGGACCGGGCAACCTGCAC
CTGATCAATGGCCTGTTTGATTGCCACCGTAACCACGTCCCTGTTCTGGCGATCGCCGCCCATATTCCGTCCAGTGAAAT
CGGCAGCGGTTATTTCCAGGAGACCCACCCGCAGGAGTTGTTCCGTGAATGCAGCCACTATTGCGAGTTGGTTTCCAGCC
CGGAGCAGATCCCGCAGGTACTCGCCATCGCCATGCGCAAAGCGGTGCTAAATCGCGGCGTCTCCGTGGTCGTACTTCCG
GGTGACGTGGCGCTGAAACCCGCGCCTGAAAACGCCGTCACCCATTGGTACCATGCCCCGCACCCCGTCGTTACGCCGGC
GGAGGAAGAGCTGAAAAAACTGGCGCAACTGCTGCGCTACTCCAGCAATATCGCATTGATGTGCGGTAGCGGCTGCGCGG
GCGCGCATGAGGAGTTGGTCGCGTTGGCGGCAAAACTAAAAGCGCCAATCGTTCATGCCCTGCGCGGCAAAGAGCACGTT
GAGTACGATAACCCTTATGATGTGGGAATGACCGGCCTGATTGGCTTCTCTTCTGGCTTCCACACCATGATGAACGCCGA
TACGCTGATCCTGCTCGGCACCCAGTTCCCCTATCGCGCCTTTTATCCGAGCGATGCCAAAATCATTCAGATTGACATCA
ACCCGGGCAGTATTGGCGCGCACAGCAAAGTCGACATGGCGCTGGTGGGCGATATTAAAGCCACGCTTCGCGCCCTGCTG
CCGCTGGTGGAAGAAAAAAGCAATCGTAAATTCCTCGATAAAGCTCTGGAACACTATCGGGACGCCCGTAAAGGGCTTGA
TGACTTAGCCAAACTCAGCGATAAAGCCATCCACCCACAATATCTGGCGCAACAAATTAGTCATTTCGCCGCTGACGACG
CTATTTTTACCTGCGATGTCGGCACGCCGACCGTCTGGGCGGCCCGCTATCTAAAAATGAACGGCAAGCGCCGCCTGCTG
GGCTCGTTTAACCACGGGTCAATGGCCAACGCCATGCCGCAGGCGCTCGGCGCTCAGGCCACCGCGCCGGGTCGTCAGGT
TATCGCGATGTGCGGTGATGGCGGCTTCAGTATGCTGATGGGCGATTTTCTCTCGGTGGTACAGATGAAGCTGCCGATAA
AAATCGTCGTCTTTAATAACAGCGTGCTGGGCTTTGTGGCGATGGAAATGAAAGCTGGCGGTTACCTTACCGACGGTACC
GAACTGCACGACACCAATTTCGCGCGTATCGCCGAAGCCTGCGGCATTACCGGTATTCGCGTGGAAAAAGCCGCAGACGT
GGACGGAGCGCTACAGCGCGCGTTTTCCATCGATGGCCCGGTACTGGTTGACGTGGTCGTGGCAAAAGAAGAGCTGGCGA
TCCCGCCGCAGATCAAACTGGAACAAGCGAAAGGCTTTAGCCTGTACATGCTGCGCGCCATTATTAGCGGCCGCGGCGAT
GAAGTGATCGAACTGGCGAAAACAAACTGGCTAAGGTAA

Upstream 100 bases:

>100_bases
CTCGCTTCATCCTTTCGCCCCTCTCTCCGCCAAAGAAAAACGTCATCGTCATCTTCAACTAAACTTGTAACCGTTATCAC
ATTTAAGGAGATGGAGAACC

Downstream 100 bases:

>100_bases
AACAGTGCACATCACCTTTTAAAATCCAGGGATGTGCCATGATTGATTTACGCAGTGATACCGTTACCCGACCGGGCCGC
GCCATGCTCGAGGCGATGAT

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MKQTVAAFIAKTLEQAGVKQIWGVTGDSLNGLSDSLNRMGTIEWMPTRHEEVAAFAAGAQAQLTGELAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
GDVALKPAPENAVTHWYHAPHPVVTPAEEELKKLAQLLRYSSNIALMCGSGCAGAHEELVALAAKLKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLILLGTQFPYRAFYPSDAKIIQIDINPGSIGAHSKVDMALVGDIKATLRALL
PLVEEKSNRKFLDKALEHYRDARKGLDDLAKLSDKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLL
GSFNHGSMANAMPQALGAQATAPGRQVIAMCGDGGFSMLMGDFLSVVQMKLPIKIVVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGITGIRVEKAADVDGALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGD
EVIELAKTNWLR

Sequences:

>Translated_572_residues
MKQTVAAFIAKTLEQAGVKQIWGVTGDSLNGLSDSLNRMGTIEWMPTRHEEVAAFAAGAQAQLTGELAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
GDVALKPAPENAVTHWYHAPHPVVTPAEEELKKLAQLLRYSSNIALMCGSGCAGAHEELVALAAKLKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLILLGTQFPYRAFYPSDAKIIQIDINPGSIGAHSKVDMALVGDIKATLRALL
PLVEEKSNRKFLDKALEHYRDARKGLDDLAKLSDKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLL
GSFNHGSMANAMPQALGAQATAPGRQVIAMCGDGGFSMLMGDFLSVVQMKLPIKIVVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGITGIRVEKAADVDGALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGD
EVIELAKTNWLR
>Mature_572_residues
MKQTVAAFIAKTLEQAGVKQIWGVTGDSLNGLSDSLNRMGTIEWMPTRHEEVAAFAAGAQAQLTGELAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
GDVALKPAPENAVTHWYHAPHPVVTPAEEELKKLAQLLRYSSNIALMCGSGCAGAHEELVALAAKLKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLILLGTQFPYRAFYPSDAKIIQIDINPGSIGAHSKVDMALVGDIKATLRALL
PLVEEKSNRKFLDKALEHYRDARKGLDDLAKLSDKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLL
GSFNHGSMANAMPQALGAQATAPGRQVIAMCGDGGFSMLMGDFLSVVQMKLPIKIVVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGITGIRVEKAADVDGALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGD
EVIELAKTNWLR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=559, Percent_Identity=24.5080500894454, Blast_Score=128, Evalue=2e-29,
Organism=Escherichia coli, GI1787096, Length=572, Percent_Identity=94.2307692307692, Blast_Score=1100, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=560, Percent_Identity=29.8214285714286, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1790104, Length=551, Percent_Identity=28.3121597096189, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI1786717, Length=548, Percent_Identity=25, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1788716, Length=543, Percent_Identity=22.8360957642726, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17531299, Length=563, Percent_Identity=26.4653641207815, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17531301, Length=563, Percent_Identity=26.4653641207815, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=559, Percent_Identity=25.2236135957066, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6320816, Length=544, Percent_Identity=22.6102941176471, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922626, Length=474, Percent_Identity=27.2151898734177, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 61708; Mature: 61708

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTVAAFIAKTLEQAGVKQIWGVTGDSLNGLSDSLNRMGTIEWMPTRHEEVAAFAAGAQ
CCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHCEEEEECCCCHHHHHHHHCCCC
AQLTGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
CEECCCEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHH
FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLPGDVALKPAPENAVTHWYHAP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCEEECCC
HPVVTPAEEELKKLAQLLRYSSNIALMCGSGCAGAHEELVALAAKLKAPIVHALRGKEHV
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
EYDNPYDVGMTGLIGFSSGFHTMMNADTLILLGTQFPYRAFYPSDAKIIQIDINPGSIGA
CCCCCCCCCCCEEEECCCCCHHHHCCCEEEEEECCCCCEEECCCCCEEEEEEECCCCCCC
HSKVDMALVGDIKATLRALLPLVEEKSNRKFLDKALEHYRDARKGLDDLAKLSDKAIHPQ
CCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHH
YLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQA
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCC
TAPGRQVIAMCGDGGFSMLMGDFLSVVQMKLPIKIVVFNNSVLGFVAMEMKAGGYLTDGT
CCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCEECCCC
ELHDTNFARIAEACGITGIRVEKAADVDGALQRAFSIDGPVLVDVVVAKEELAIPPQIKL
CCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCCCCH
EQAKGFSLYMLRAIISGRGDEVIELAKTNWLR
HCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKQTVAAFIAKTLEQAGVKQIWGVTGDSLNGLSDSLNRMGTIEWMPTRHEEVAAFAAGAQ
CCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHCEEEEECCCCHHHHHHHHCCCC
AQLTGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
CEECCCEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHH
FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLPGDVALKPAPENAVTHWYHAP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCEEECCC
HPVVTPAEEELKKLAQLLRYSSNIALMCGSGCAGAHEELVALAAKLKAPIVHALRGKEHV
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
EYDNPYDVGMTGLIGFSSGFHTMMNADTLILLGTQFPYRAFYPSDAKIIQIDINPGSIGA
CCCCCCCCCCCEEEECCCCCHHHHCCCEEEEEECCCCCEEECCCCCEEEEEEECCCCCCC
HSKVDMALVGDIKATLRALLPLVEEKSNRKFLDKALEHYRDARKGLDDLAKLSDKAIHPQ
CCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHH
YLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQA
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCC
TAPGRQVIAMCGDGGFSMLMGDFLSVVQMKLPIKIVVFNNSVLGFVAMEMKAGGYLTDGT
CCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEECCCCEECCCC
ELHDTNFARIAEACGITGIRVEKAADVDGALQRAFSIDGPVLVDVVVAKEELAIPPQIKL
CCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCCCCH
EQAKGFSLYMLRAIISGRGDEVIELAKTNWLR
HCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]