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The map label for this gene is ansA [H]

Identifier: 29141639

GI number: 29141639

Start: 1239676

End: 1240692

Strand: Reverse

Name: ansA [H]

Synonym: t1173

Alternate gene names: 29141639

Gene position: 1240692-1239676 (Counterclockwise)

Preceding gene: 29141640

Following gene: 29141638

Centisome position: 25.89

GC content: 52.61

Gene sequence:

>1017_bases
ATGCAAAAGAAATCAATTTACGTTGCCTATACCGGCGGTACTATCGGTATGCAGCGCTCCGATAAAGGGTATATCCCGGT
GTCCGGGCGCCTTCAGCGTCAGCTGGCGCTGATGCCGGAATTCCACCGCCCGGAGATGCCGGATTTTACCATCCATGAAT
ATGCCCCGCTGATGGATTCATCAGATATGACGCCGGAAGACTGGCAGCATATCGCTGCAGATATCAAAGCGCACTACGAT
GAGTATGACGGTTTTGTTATTCTGCACGGCACCGACACGATGGCGTATACCGCATCGGCGCTCTCTTTCATGCTGGAAAA
TCTGGGTAAACCGGTTATTGTGACAGGGTCACAAATTCCGCTGGCTGAGCTGCGCTCCGACGGGCAAATTAATTTGCTGA
ATGCGCTTTATGTCGCCGCCAACTACCCTGTTAATGAAGTAACGCTGTTTTTTAATAATCGTCTGTTCCGCGGCAATCGC
ACGACCAAAGCACATGCGGATGGTTTTGACGCTTTTGCCTCTCCTAATCTGCCGCCCTTGCTGGAAGCAGGAATTCACAT
TCGCCGTTTAAATACGCCTCCCGCGCCCTACGGTTCTGGTGAATTAATCGTTCACCCTATTACGCCGCAGCCGATAGGCG
TAGTGACGATTTATCCGGGGATTTCCGCCGAGGTAGTACGTAACTTTCTGCGTCAGCCGGTAAAAGCGCTGATTCTGCGC
TCTTATGGCGTAGGCAACGCGCCGCAAAATAAAGCGTTTTTACAGGAGCTGAAAGAGGCCAGTTCGCGCGGCATCGTGGT
GGTTAACCTGACGCAATGCATGTCAGGGCGAGTCAATATGGGCGGGTACGCTACCGGTAATGCGCTGGCGCACGCTGGCG
TGATTGGCGGTGCCGATATGACCGTCGAAGCTACCTTAACCAAACTGCATTATTTGTTAAGCCAGGGTCTGGACACTCAG
GCGATTCGTAGCGCGATGGCGCAAAACTTGCGTGGTGAATTGACGCCGGATGATTAA

Upstream 100 bases:

>100_bases
GCTAATGCTTCATCAATAGCCATACCGGATGTCGGCGTCTGCTCATAGCCGCCATCCGATAATCATTAATACGTCCTTCG
CGACACGACTGAACATTATC

Downstream 100 bases:

>100_bases
AAAATCCCGACGACAAGGAGAAGATAATGACAAACCGGGCCTTATTACTGGTCGACTTGCAAAATGACTTTTGTGCCGGC
GGCGCGCTGGCTGTAGCGGA

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MQKKSIYVAYTGGTIGMQRSDKGYIPVSGRLQRQLALMPEFHRPEMPDFTIHEYAPLMDSSDMTPEDWQHIAADIKAHYD
EYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPVNEVTLFFNNRLFRGNR
TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPYGSGELIVHPITPQPIGVVTIYPGISAEVVRNFLRQPVKALILR
SYGVGNAPQNKAFLQELKEASSRGIVVVNLTQCMSGRVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQGLDTQ
AIRSAMAQNLRGELTPDD

Sequences:

>Translated_338_residues
MQKKSIYVAYTGGTIGMQRSDKGYIPVSGRLQRQLALMPEFHRPEMPDFTIHEYAPLMDSSDMTPEDWQHIAADIKAHYD
EYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPVNEVTLFFNNRLFRGNR
TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPYGSGELIVHPITPQPIGVVTIYPGISAEVVRNFLRQPVKALILR
SYGVGNAPQNKAFLQELKEASSRGIVVVNLTQCMSGRVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQGLDTQ
AIRSAMAQNLRGELTPDD
>Mature_338_residues
MQKKSIYVAYTGGTIGMQRSDKGYIPVSGRLQRQLALMPEFHRPEMPDFTIHEYAPLMDSSDMTPEDWQHIAADIKAHYD
EYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPVNEVTLFFNNRLFRGNR
TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPYGSGELIVHPITPQPIGVVTIYPGISAEVVRNFLRQPVKALILR
SYGVGNAPQNKAFLQELKEASSRGIVVVNLTQCMSGRVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQGLDTQ
AIRSAMAQNLRGELTPDD

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=363, Percent_Identity=45.1790633608815, Blast_Score=278, Evalue=5e-75,
Organism=Escherichia coli, GI1788065, Length=338, Percent_Identity=94.3786982248521, Blast_Score=666, Evalue=0.0,
Organism=Escherichia coli, GI1789327, Length=283, Percent_Identity=27.9151943462898, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=360, Percent_Identity=45.2777777777778, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI193207374, Length=363, Percent_Identity=45.4545454545455, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17558280, Length=363, Percent_Identity=45.4545454545455, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17558278, Length=363, Percent_Identity=45.4545454545455, Blast_Score=295, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6320527, Length=176, Percent_Identity=29.5454545454545, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24639609, Length=368, Percent_Identity=42.9347826086957, Blast_Score=281, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24645530, Length=362, Percent_Identity=42.5414364640884, Blast_Score=274, Evalue=6e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36867; Mature: 36867

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKSIYVAYTGGTIGMQRSDKGYIPVSGRLQRQLALMPEFHRPEMPDFTIHEYAPLMDS
CCCCEEEEEEECCEEEECCCCCCEECCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCC
SDMTPEDWQHIAADIKAHYDEYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIP
CCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
LAELRSDGQINLLNALYVAANYPVNEVTLFFNNRLFRGNRTTKAHADGFDAFASPNLPPL
HHHHCCCCCEEEEEEEEEEECCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCHH
LEAGIHIRRLNTPPAPYGSGELIVHPITPQPIGVVTIYPGISAEVVRNFLRQPVKALILR
HHCCEEEEECCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
SYGVGNAPQNKAFLQELKEASSRGIVVVNLTQCMSGRVNMGGYATGNALAHAGVIGGADM
HCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCEECCCHHHHCCEECCCCE
TVEATLTKLHYLLSQGLDTQAIRSAMAQNLRGELTPDD
EHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQKKSIYVAYTGGTIGMQRSDKGYIPVSGRLQRQLALMPEFHRPEMPDFTIHEYAPLMDS
CCCCEEEEEEECCEEEECCCCCCEECCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCC
SDMTPEDWQHIAADIKAHYDEYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIP
CCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
LAELRSDGQINLLNALYVAANYPVNEVTLFFNNRLFRGNRTTKAHADGFDAFASPNLPPL
HHHHCCCCCEEEEEEEEEEECCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCHH
LEAGIHIRRLNTPPAPYGSGELIVHPITPQPIGVVTIYPGISAEVVRNFLRQPVKALILR
HHCCEEEEECCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
SYGVGNAPQNKAFLQELKEASSRGIVVVNLTQCMSGRVNMGGYATGNALAHAGVIGGADM
HCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCEECCCHHHHCCEECCCCE
TVEATLTKLHYLLSQGLDTQAIRSAMAQNLRGELTPDD
EHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]