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The map label for this gene is pncA [H]

Identifier: 29141638

GI number: 29141638

Start: 1238993

End: 1239649

Strand: Reverse

Name: pncA [H]

Synonym: t1172

Alternate gene names: 29141638

Gene position: 1239649-1238993 (Counterclockwise)

Preceding gene: 29141639

Following gene: 29141635

Centisome position: 25.87

GC content: 57.38

Gene sequence:

>657_bases
ATGACAAACCGGGCCTTATTACTGGTCGACTTGCAAAATGACTTTTGTGCCGGCGGCGCGCTGGCTGTAGCGGAAGGCGA
CAGCACCATCGACATCGCCAACGCTCTTATTGACTGGTGCCAGCCCCGGCAGATTCCGGTGCTGGCCAGCCAGGACTGGC
ATCCCGCGCAGCATGGCAGCTTCGCCAGCCAGCATCAGGCGGAACCCTATAGACAGGGAAAGCTGGACGGGCTGCCGCAA
ACGCTCTGGCCGGATCACTGCGTTCAACATACCGACGGCGCGGCGTTACATCCCCTGCTTAACCAACATGCGATTGACGC
CTGCATCTATAAAGGTGAAAACCCGCTGATTGATAGCTACAGCGCGTTTTTTGACAACGAACATCGGCAGAAAACTACCC
TTGATACCTGGCTTCGCGAGCATGACGTTACCGAGCTTATCGTGATGGGGTTGGCGACCGATTACTGCGTAAAATTTACG
GTGCTGGATGCGCTAGAGTTGGGCTATGCCGTTAACGTTATCACCGATGGCTGTCGCGGCGTGAATATTCACCCGCAGGA
TAGCGCACACGCGTTTATGGAGATGGCGGCGGCAGGCGCGACGCTGTATACGCTGGCAGACTGGGAAGAGACGCAGGGCC
AGGCTATCGCCAGGTAG

Upstream 100 bases:

>100_bases
AGGGTCTGGACACTCAGGCGATTCGTAGCGCGATGGCGCAAAACTTGCGTGGTGAATTGACGCCGGATGATTAAAAAATC
CCGACGACAAGGAGAAGATA

Downstream 100 bases:

>100_bases
CGGAGCAGGTATCGTATCCGGTCAATACATCACGCAACGGTGAGACCATTGCATAGATCTTTGGGCCGGATACGTCACGT
CAGCGTCGCTACCAGGCAAA

Product: nicotinamidase/pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTNRALLLVDLQNDFCAGGALAVAEGDSTIDIANALIDWCQPRQIPVLASQDWHPAQHGSFASQHQAEPYRQGKLDGLPQ
TLWPDHCVQHTDGAALHPLLNQHAIDACIYKGENPLIDSYSAFFDNEHRQKTTLDTWLREHDVTELIVMGLATDYCVKFT
VLDALELGYAVNVITDGCRGVNIHPQDSAHAFMEMAAAGATLYTLADWEETQGQAIAR

Sequences:

>Translated_218_residues
MTNRALLLVDLQNDFCAGGALAVAEGDSTIDIANALIDWCQPRQIPVLASQDWHPAQHGSFASQHQAEPYRQGKLDGLPQ
TLWPDHCVQHTDGAALHPLLNQHAIDACIYKGENPLIDSYSAFFDNEHRQKTTLDTWLREHDVTELIVMGLATDYCVKFT
VLDALELGYAVNVITDGCRGVNIHPQDSAHAFMEMAAAGATLYTLADWEETQGQAIAR
>Mature_217_residues
TNRALLLVDLQNDFCAGGALAVAEGDSTIDIANALIDWCQPRQIPVLASQDWHPAQHGSFASQHQAEPYRQGKLDGLPQT
LWPDHCVQHTDGAALHPLLNQHAIDACIYKGENPLIDSYSAFFDNEHRQKTTLDTWLREHDVTELIVMGLATDYCVKFTV
LDALELGYAVNVITDGCRGVNIHPQDSAHAFMEMAAAGATLYTLADWEETQGQAIAR

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=213, Percent_Identity=80.7511737089202, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI193206735, Length=216, Percent_Identity=34.2592592592593, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17543130, Length=216, Percent_Identity=34.2592592592593, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71999339, Length=214, Percent_Identity=32.7102803738318, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6321401, Length=201, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648247, Length=204, Percent_Identity=37.7450980392157, Blast_Score=118, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 24008; Mature: 23877

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRALLLVDLQNDFCAGGALAVAEGDSTIDIANALIDWCQPRQIPVLASQDWHPAQHGS
CCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCC
FASQHQAEPYRQGKLDGLPQTLWPDHCVQHTDGAALHPLLNQHAIDACIYKGENPLIDSY
CCHHHCCCCHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCHHHH
SAFFDNEHRQKTTLDTWLREHDVTELIVMGLATDYCVKFTVLDALELGYAVNVITDGCRG
HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCC
VNIHPQDSAHAFMEMAAAGATLYTLADWEETQGQAIAR
CCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TNRALLLVDLQNDFCAGGALAVAEGDSTIDIANALIDWCQPRQIPVLASQDWHPAQHGS
CCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCC
FASQHQAEPYRQGKLDGLPQTLWPDHCVQHTDGAALHPLLNQHAIDACIYKGENPLIDSY
CCHHHCCCCHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCHHHH
SAFFDNEHRQKTTLDTWLREHDVTELIVMGLATDYCVKFTVLDALELGYAVNVITDGCRG
HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCC
VNIHPQDSAHAFMEMAAAGATLYTLADWEETQGQAIAR
CCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]