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The map label for this gene is wcaI [H]

Identifier: 29141267

GI number: 29141267

Start: 854809

End: 856032

Strand: Direct

Name: wcaI [H]

Synonym: t0766

Alternate gene names: 29141267

Gene position: 854809-856032 (Clockwise)

Preceding gene: 29141266

Following gene: 29141268

Centisome position: 17.84

GC content: 57.68

Gene sequence:

>1224_bases
ATGAAGATCCTGGTCTATGGCATTAACTATTCACCGGAACTGACCGGTATCGGCAAATATACCGGCGAAATGGTGGCGTG
GATGGCGCGGGAAGGCCATGAGGTGCGGGTTATTACTGCGCCGCCGTACTACCCGCAGTGGAAGGTCGGTGAGCGCTATT
CCGCCTGGCGCTATCGCCGGGAAGAGGGCGAGGCTACCGTCTGGCGCTGCCCGCTGTATGTGCCGAAACAGCCCTCCACC
CTAAAACGACTGCTTCATCTGGGGAGCTTTGCATTGAGCAGTTTTTTCCCACTGATGGCGCAGCGTCGCTGGAAGCCGGA
TCGGATTATCGGCGTTGTACCGACGCTCTTTTGTACGCCGGGAATGCGTCTGCTGGCGACACTCTCCGGCGCGCGTACCG
TACTGCATATTCAGGATTACGAAGTGGACGCCATGCTCGGCTTAGGAATGGCGGGAAAAGGCAAGCGCGGCAGCGTAGCG
CGGTTAGCGACGGCCTTTGAACGCAGCGCGCTGCGTAACGTTGATAATGTTTCGACCATCTCTCGCTCCATGATGAATAA
AGCGCGGGAAAAGGGCGTCGCGGCGGAAAAAATCCTCTTTTTTCCAAACTGGTCGGAAGTGGCGCGCTTTCAGGACGTTA
ATGACGCTGACGTGACGGCTTTGCGTCAGCAGCTTGGCTTGCCAGAGGGCAAAAAAATCGTGCTCTATTCCGGCAATATC
GGCGAAAAACAAGGGCTGGAAAAGGTGATTGACGCCGCAGAACGATTACGCGATCGACCGTTGATTTTTGCGATTGTCGG
CCAGGGGGGCGGCAAGGCGCGGCTGGAGAACATGGCCCGCGAACGCGGCCTGCTAAATATTAAATTCCTCCCGCTGCAGC
CTTACGACGCCTTACCCGCGCTGCTGAAAATGGGCGACTGCCATCTGGTGGTGCAAAAGCGCGGCGCAGCGGATGCGGTA
CTGCCCTCCAAGCTGACTAACATTCTGGCGGTCGGCGGTAATGCGGTGATTACCGCTGAGCCGCACACCGAATTAGGACA
ACTTTGCGCGCGCTATCCGGGCATCGCCGTTTGCGTGGAGCCGGAGTCGACCGACGCGCTGGTCGACGGGATTAGCCAGG
CGCTCGCCATGCCGAAAAACAACACGACGGCACGTGAATACGCCGAACGCACGCTCAATAAAGAGAACGTGCTACGCCAA
TTTATTGCAGATATTCGGGGATGA

Upstream 100 bases:

>100_bases
GCAGTTACCGCTGGCTGACGCCGGAACAGCTTCTGGCAGGCGACAATGTTCATGAGAACAGTCGGGCCTACTTCTCTCCT
GACGCGCCGGCGGTGGGCCT

Downstream 100 bases:

>100_bases
TAATGCGTCAGACAAAACTTTACCCGGTTGTGATGGCCGGTGGCTCCGGTAGCCGATTGTGGCCGCTTTCTCGCGTGCTT
TATCCAAAACAGTTTTTGTG

Product: glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MKILVYGINYSPELTGIGKYTGEMVAWMAREGHEVRVITAPPYYPQWKVGERYSAWRYRREEGEATVWRCPLYVPKQPST
LKRLLHLGSFALSSFFPLMAQRRWKPDRIIGVVPTLFCTPGMRLLATLSGARTVLHIQDYEVDAMLGLGMAGKGKRGSVA
RLATAFERSALRNVDNVSTISRSMMNKAREKGVAAEKILFFPNWSEVARFQDVNDADVTALRQQLGLPEGKKIVLYSGNI
GEKQGLEKVIDAAERLRDRPLIFAIVGQGGGKARLENMARERGLLNIKFLPLQPYDALPALLKMGDCHLVVQKRGAADAV
LPSKLTNILAVGGNAVITAEPHTELGQLCARYPGIAVCVEPESTDALVDGISQALAMPKNNTTAREYAERTLNKENVLRQ
FIADIRG

Sequences:

>Translated_407_residues
MKILVYGINYSPELTGIGKYTGEMVAWMAREGHEVRVITAPPYYPQWKVGERYSAWRYRREEGEATVWRCPLYVPKQPST
LKRLLHLGSFALSSFFPLMAQRRWKPDRIIGVVPTLFCTPGMRLLATLSGARTVLHIQDYEVDAMLGLGMAGKGKRGSVA
RLATAFERSALRNVDNVSTISRSMMNKAREKGVAAEKILFFPNWSEVARFQDVNDADVTALRQQLGLPEGKKIVLYSGNI
GEKQGLEKVIDAAERLRDRPLIFAIVGQGGGKARLENMARERGLLNIKFLPLQPYDALPALLKMGDCHLVVQKRGAADAV
LPSKLTNILAVGGNAVITAEPHTELGQLCARYPGIAVCVEPESTDALVDGISQALAMPKNNTTAREYAERTLNKENVLRQ
FIADIRG
>Mature_407_residues
MKILVYGINYSPELTGIGKYTGEMVAWMAREGHEVRVITAPPYYPQWKVGERYSAWRYRREEGEATVWRCPLYVPKQPST
LKRLLHLGSFALSSFFPLMAQRRWKPDRIIGVVPTLFCTPGMRLLATLSGARTVLHIQDYEVDAMLGLGMAGKGKRGSVA
RLATAFERSALRNVDNVSTISRSMMNKAREKGVAAEKILFFPNWSEVARFQDVNDADVTALRQQLGLPEGKKIVLYSGNI
GEKQGLEKVIDAAERLRDRPLIFAIVGQGGGKARLENMARERGLLNIKFLPLQPYDALPALLKMGDCHLVVQKRGAADAV
LPSKLTNILAVGGNAVITAEPHTELGQLCARYPGIAVCVEPESTDALVDGISQALAMPKNNTTAREYAERTLNKENVLRQ
FIADIRG

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788363, Length=407, Percent_Identity=85.995085995086, Blast_Score=706, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 45064; Mature: 45064

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVYGINYSPELTGIGKYTGEMVAWMAREGHEVRVITAPPYYPQWKVGERYSAWRYRR
CEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
EEGEATVWRCPLYVPKQPSTLKRLLHLGSFALSSFFPLMAQRRWKPDRIIGVVPTLFCTP
HCCCEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHCC
GMRLLATLSGARTVLHIQDYEVDAMLGLGMAGKGKRGSVARLATAFERSALRNVDNVSTI
CHHHHHHHCCCEEEEEEECCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
SRSMMNKAREKGVAAEKILFFPNWSEVARFQDVNDADVTALRQQLGLPEGKKIVLYSGNI
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCC
GEKQGLEKVIDAAERLRDRPLIFAIVGQGGGKARLENMARERGLLNIKFLPLQPYDALPA
CCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHH
LLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGNAVITAEPHTELGQLCARYPGIAVCVE
HHHCCCEEEEEECCCCCCHHHHHHHHHHEEECCCEEEEECCCHHHHHHHHHCCCEEEEEC
PESTDALVDGISQALAMPKNNTTAREYAERTLNKENVLRQFIADIRG
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILVYGINYSPELTGIGKYTGEMVAWMAREGHEVRVITAPPYYPQWKVGERYSAWRYRR
CEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
EEGEATVWRCPLYVPKQPSTLKRLLHLGSFALSSFFPLMAQRRWKPDRIIGVVPTLFCTP
HCCCEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHCC
GMRLLATLSGARTVLHIQDYEVDAMLGLGMAGKGKRGSVARLATAFERSALRNVDNVSTI
CHHHHHHHCCCEEEEEEECCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
SRSMMNKAREKGVAAEKILFFPNWSEVARFQDVNDADVTALRQQLGLPEGKKIVLYSGNI
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCC
GEKQGLEKVIDAAERLRDRPLIFAIVGQGGGKARLENMARERGLLNIKFLPLQPYDALPA
CCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHH
LLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGNAVITAEPHTELGQLCARYPGIAVCVE
HHHCCCEEEEEECCCCCCHHHHHHHHHHEEECCCEEEEECCCHHHHHHHHHCCCEEEEEC
PESTDALVDGISQALAMPKNNTTAREYAERTLNKENVLRQFIADIRG
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503 [H]