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The map label for this gene is wcaH [H]

Identifier: 29141266

GI number: 29141266

Start: 854333

End: 854812

Strand: Direct

Name: wcaH [H]

Synonym: t0764

Alternate gene names: 29141266

Gene position: 854333-854812 (Clockwise)

Preceding gene: 29141265

Following gene: 29141267

Centisome position: 17.83

GC content: 60.21

Gene sequence:

>480_bases
GTGACAATGTTTTTACGTCAGGAAGATTTCGCCGCCGTGGTGCGGACCACGCCCCTCATCTCCCTCGATTTCATCGTGGA
AAACGGCCAGGGGGAAATTTTGCTGGGCCAGCGCCTCAACCGTCCGGCGCAGGGCTACTGGTTTGTGCCGGGGGGGCGGG
TGTGCAAAGACGAAACGCTGGAGGCCGCTTTTGCACGCCTGACGCAGGCGGAACTGGGCGTGCGTCTGCCGCTGGCGGCA
GGGACGTTTTATGGCGTCTGGCAGCACTTCTATGACGACAACTTTTCCAGTGAGGATTTTTCAACTCACTACATCGTGCT
CGGCTTTCGTCTGCGCGTGGCGGAGAGCGATTTACGCCTGCCTGATGCCCAGCATGGCAGTTACCGCTGGCTGACGCCGG
AACAGCTTCTGGCAGGCGACAATGTTCATGAGAACAGTCGGGCCTACTTCTCTCCTGACGCGCCGGCGGTGGGCCTATGA

Upstream 100 bases:

>100_bases
CGCGCCTGCATCAGCTTGGCTGGTATCACGAAATTTCACTGGAGGCAGGGCTTGCCGGTACTTACCAGTGGTTCCTTGAG
AATCAGCAACGGTTCCGGGG

Downstream 100 bases:

>100_bases
AGATCCTGGTCTATGGCATTAACTATTCACCGGAACTGACCGGTATCGGCAAATATACCGGCGAAATGGTGGCGTGGATG
GCGCGGGAAGGCCATGAGGT

Product: O-antigen biosynthesis protein

Products: GDP; mannose [C]

Alternate protein names: GDPMH; Colanic acid biosynthesis protein wcaH [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MTMFLRQEDFAAVVRTTPLISLDFIVENGQGEILLGQRLNRPAQGYWFVPGGRVCKDETLEAAFARLTQAELGVRLPLAA
GTFYGVWQHFYDDNFSSEDFSTHYIVLGFRLRVAESDLRLPDAQHGSYRWLTPEQLLAGDNVHENSRAYFSPDAPAVGL

Sequences:

>Translated_159_residues
MTMFLRQEDFAAVVRTTPLISLDFIVENGQGEILLGQRLNRPAQGYWFVPGGRVCKDETLEAAFARLTQAELGVRLPLAA
GTFYGVWQHFYDDNFSSEDFSTHYIVLGFRLRVAESDLRLPDAQHGSYRWLTPEQLLAGDNVHENSRAYFSPDAPAVGL
>Mature_158_residues
TMFLRQEDFAAVVRTTPLISLDFIVENGQGEILLGQRLNRPAQGYWFVPGGRVCKDETLEAAFARLTQAELGVRLPLAAG
TFYGVWQHFYDDNFSSEDFSTHYIVLGFRLRVAESDLRLPDAQHGSYRWLTPEQLLAGDNVHENSRAYFSPDAPAVGL

Specific function: It could participate in the regulation of cell wall biosynthesis by influencing the concentration of GDP-mannose or GDP-glucose in the cell. May be involved in the degradation of GDP-mannose and GDP-glucose, diverting the GDP to the synthesis of GDP-fucos

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI87082031, Length=148, Percent_Identity=78.3783783783784, Blast_Score=249, Evalue=9e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021161
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: NA

Molecular weight: Translated: 17912; Mature: 17781

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMFLRQEDFAAVVRTTPLISLDFIVENGQGEILLGQRLNRPAQGYWFVPGGRVCKDETL
CCEEECCCCHHEEECCCCEEEEEEEEECCCCCEEECCCCCCCCCCEEECCCCCCCCHHHH
EAAFARLTQAELGVRLPLAAGTFYGVWQHFYDDNFSSEDFSTHYIVLGFRLRVAESDLRL
HHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCCC
PDAQHGSYRWLTPEQLLAGDNVHENSRAYFSPDAPAVGL
CCCCCCCEEEECHHHHHCCCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure 
TMFLRQEDFAAVVRTTPLISLDFIVENGQGEILLGQRLNRPAQGYWFVPGGRVCKDETL
CEEECCCCHHEEECCCCEEEEEEEEECCCCCEEECCCCCCCCCCEEECCCCCCCCHHHH
EAAFARLTQAELGVRLPLAAGTFYGVWQHFYDDNFSSEDFSTHYIVLGFRLRVAESDLRL
HHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCCC
PDAQHGSYRWLTPEQLLAGDNVHENSRAYFSPDAPAVGL
CCCCCCCEEEECHHHHHCCCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-mannose; H2O [C]

Specific reaction: GDP-mannose + H2O = GDP + mannose [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]