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The map label for this gene is xapA [H]

Identifier: 29140962

GI number: 29140962

Start: 508412

End: 509245

Strand: Direct

Name: xapA [H]

Synonym: t0435

Alternate gene names: 29140962

Gene position: 508412-509245 (Clockwise)

Preceding gene: 29140960

Following gene: 29140963

Centisome position: 10.61

GC content: 53.24

Gene sequence:

>834_bases
ATGCCTCACGCTCTTTTTTCTCATAATCCTGAGTATTGTATTGATATTATTCATGCACATAAACCGGCATTTACCCCGCG
AGTCGCGTTTATTTTAGGCTCCGGGTTGGGTGCTCTGGCGGATCAAATCGAAGATGCGGTAGCCATTTCTTACGAGAAAT
TGCCGGGATTTCCCGTCAGCACCGTACACGGCCATGCTGGTGAACTGGTGCTCGGCCATCTGGCGGGTGTTCCGGTGGCC
TGTATGAAAGGCCGCGGACATTTTTATGAAGGGCGTGGAATGGCGGTGATGACCGATGCCATTCGCACGTTGAAATTACT
GGGCTGTGAAGTGTTGTTCTGCACCAATGCGGCAGGATCTCTGCGTCCGGAAGTGGGGCCGGGTAGCCTGGTTGCGCTTA
GCGATCACATTAATACGATGCCCGGTACGCCGATGGTGGGGCCAAACGATGATCGTTATGGCGATCGCTTCTTCTCGCTG
GCTAATGCTTATGACGCAGATTATCGCGCCATGCTTCAGTCCGTTGCGGCAGAGAAGGGATTCCCCCTCACTGAAGGCGT
ATTTGTTTCTTATCCGGGTCCGAACTTCGAAACGGCGGCAGAAATCCGCATGATGCAGATTATTGGCGGCGATGTGGTCG
GAATGTCCGTTGTCCCTGAGGTGATTAGCGCACGTCATTGTGGCCTCAAAGTTGTTGCCGTCTCAGCGATCACCAATCTG
GCGGAAGGGCTGGGCGATGTAAAACTCTCCCATGCGCAAACGCTGGCGGCGGCTGAACTTTCCCGGCAGAACTTTATCAA
TCTGATTTGCGGCTTTTTACGTAAGCTGGCCTAA

Upstream 100 bases:

>100_bases
TAAATGGCTATTGATGACACGTGAAAAGTGTATTGGTCAGCAAGGGGCGATGGTCGTTATAAAAAGGAGTCAAATTAAAT
CCGATAGAACAAGGAAACTT

Downstream 100 bases:

>100_bases
ACACAATGACTACGGCCCCGTATTGGGGCTTTATCTCACGGCAAGGAATAAAAAATGGGTATTACGTCCCGCTTAAAAGT
CATGTCGTTCTTGCAATATT

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MPHALFSHNPEYCIDIIHAHKPAFTPRVAFILGSGLGALADQIEDAVAISYEKLPGFPVSTVHGHAGELVLGHLAGVPVA
CMKGRGHFYEGRGMAVMTDAIRTLKLLGCEVLFCTNAAGSLRPEVGPGSLVALSDHINTMPGTPMVGPNDDRYGDRFFSL
ANAYDADYRAMLQSVAAEKGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVVPEVISARHCGLKVVAVSAITNL
AEGLGDVKLSHAQTLAAAELSRQNFINLICGFLRKLA

Sequences:

>Translated_277_residues
MPHALFSHNPEYCIDIIHAHKPAFTPRVAFILGSGLGALADQIEDAVAISYEKLPGFPVSTVHGHAGELVLGHLAGVPVA
CMKGRGHFYEGRGMAVMTDAIRTLKLLGCEVLFCTNAAGSLRPEVGPGSLVALSDHINTMPGTPMVGPNDDRYGDRFFSL
ANAYDADYRAMLQSVAAEKGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVVPEVISARHCGLKVVAVSAITNL
AEGLGDVKLSHAQTLAAAELSRQNFINLICGFLRKLA
>Mature_276_residues
PHALFSHNPEYCIDIIHAHKPAFTPRVAFILGSGLGALADQIEDAVAISYEKLPGFPVSTVHGHAGELVLGHLAGVPVAC
MKGRGHFYEGRGMAVMTDAIRTLKLLGCEVLFCTNAAGSLRPEVGPGSLVALSDHINTMPGTPMVGPNDDRYGDRFFSLA
NAYDADYRAMLQSVAAEKGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVVPEVISARHCGLKVVAVSAITNLA
EGLGDVKLSHAQTLAAAELSRQNFINLICGFLRKLA

Specific function: The nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the nucleoside molecule, with the formation of the corresponding free bases and pentose-1-phosphate. This protein can degrade all purine nucleosides except ade

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=252, Percent_Identity=44.4444444444444, Blast_Score=201, Evalue=6e-52,
Organism=Homo sapiens, GI47132622, Length=176, Percent_Identity=28.4090909090909, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788746, Length=277, Percent_Identity=88.4476534296029, Blast_Score=509, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17541190, Length=239, Percent_Identity=38.4937238493724, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6323238, Length=275, Percent_Identity=39.6363636363636, Blast_Score=175, Evalue=9e-45,
Organism=Drosophila melanogaster, GI24656090, Length=285, Percent_Identity=40, Blast_Score=204, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24656093, Length=260, Percent_Identity=41.9230769230769, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI45552887, Length=260, Percent_Identity=41.9230769230769, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI45552885, Length=260, Percent_Identity=41.9230769230769, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24762376, Length=236, Percent_Identity=33.0508474576271, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR010943
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: 2.4.2.-

Molecular weight: Translated: 29373; Mature: 29242

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHALFSHNPEYCIDIIHAHKPAFTPRVAFILGSGLGALADQIEDAVAISYEKLPGFPVS
CCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
TVHGHAGELVLGHLAGVPVACMKGRGHFYEGRGMAVMTDAIRTLKLLGCEVLFCTNAAGS
CCCCCCCHHHHHHHCCCCEEEECCCCCEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCC
LRPEVGPGSLVALSDHINTMPGTPMVGPNDDRYGDRFFSLANAYDADYRAMLQSVAAEKG
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCC
FPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVVPEVISARHCGLKVVAVSAITNL
CCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHH
AEGLGDVKLSHAQTLAAAELSRQNFINLICGFLRKLA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PHALFSHNPEYCIDIIHAHKPAFTPRVAFILGSGLGALADQIEDAVAISYEKLPGFPVS
CCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
TVHGHAGELVLGHLAGVPVACMKGRGHFYEGRGMAVMTDAIRTLKLLGCEVLFCTNAAGS
CCCCCCCHHHHHHHCCCCEEEECCCCCEECCCCCCHHHHHHHHHHHHCCEEEEEECCCCC
LRPEVGPGSLVALSDHINTMPGTPMVGPNDDRYGDRFFSLANAYDADYRAMLQSVAAEKG
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCC
FPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVVPEVISARHCGLKVVAVSAITNL
CCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHH
AEGLGDVKLSHAQTLAAAELSRQNFINLICGFLRKLA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559336; 9205837; 9278503 [H]