Click here to switch to the map view.

The map label for this gene is yfiG [H]

Identifier: 29140796

GI number: 29140796

Start: 300176

End: 300595

Strand: Reverse

Name: yfiG [H]

Synonym: t0261

Alternate gene names: 29140796

Gene position: 300595-300176 (Counterclockwise)

Preceding gene: 29140798

Following gene: 29140795

Centisome position: 6.27

GC content: 53.1

Gene sequence:

>420_bases
ATGAACACCGTTTGTACCCATTGTCAGGCCATTAACCGCATCCCCGGCGATCGGCTTCAGGACGCGGCAAAATGTGGACG
CTGCGGTCATGAGTTGTTTGACGGAGAAGTCATTAATGCGACCGGCGAAACGCTGGACAAACTGTTGAAGGACGATCTTC
CAGTGGTGATCGACTTTTGGGCACCCTGGTGCGGCCCTTGCCGTAACTTTGCGCCAATCTTCGAGGATGTCGCAGAAGAG
CGCAGCGGTAAAGTCCGTTTCGTCAAAGTAAATACCGAGGCGGAACGGGAACTCAGCGCTCGTTTCGGTATCCGCAGCAT
CCCGATGATTATGATTTTTAAACATGGACAGGTGGTCGATATGCTTAATGGCGCAGTGCCTAAAGCGCCTTTCGACAGCT
GGCTGAACGAAGCGCTGTAA

Upstream 100 bases:

>100_bases
AAACACATTGTTGTTTAGCACAGACTCCTCGATAATATATAACATTATAGAAACATCCCGCGCGTAGCGGGACGTCTTCC
GACGTATTCAGAGGTTAGCT

Downstream 100 bases:

>100_bases
ACGCCACGGGGCGCATCTTGTGCCCCGTTTTCACCTCTGCCACAATAGCGTTTTTCTCCGCAAACGCTTTCTATGACCAA
TAACGCTGTCCTTCAGTTAC

Product: thioredoxin 2

Products: NA

Alternate protein names: Trx-2; Protein-disulfide reductase [H]

Number of amino acids: Translated: 139; Mature: 139

Protein sequence:

>139_residues
MNTVCTHCQAINRIPGDRLQDAAKCGRCGHELFDGEVINATGETLDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAEE
RSGKVRFVKVNTEAERELSARFGIRSIPMIMIFKHGQVVDMLNGAVPKAPFDSWLNEAL

Sequences:

>Translated_139_residues
MNTVCTHCQAINRIPGDRLQDAAKCGRCGHELFDGEVINATGETLDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAEE
RSGKVRFVKVNTEAERELSARFGIRSIPMIMIFKHGQVVDMLNGAVPKAPFDSWLNEAL
>Mature_139_residues
MNTVCTHCQAINRIPGDRLQDAAKCGRCGHELFDGEVINATGETLDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAEE
RSGKVRFVKVNTEAERELSARFGIRSIPMIMIFKHGQVVDMLNGAVPKAPFDSWLNEAL

Specific function: Efficient electron donor for the essential enzyme ribonucleotide reductase. Is also able to reduce the interchain disulfide bridges of insulin [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Homo sapiens, GI4758304, Length=83, Percent_Identity=36.144578313253, Blast_Score=68, Evalue=3e-12,
Organism=Homo sapiens, GI21361403, Length=94, Percent_Identity=29.7872340425532, Blast_Score=67, Evalue=3e-12,
Organism=Homo sapiens, GI24308127, Length=68, Percent_Identity=44.1176470588235, Blast_Score=67, Evalue=6e-12,
Organism=Homo sapiens, GI5031973, Length=87, Percent_Identity=36.7816091954023, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1788936, Length=139, Percent_Identity=93.5251798561151, Blast_Score=275, Evalue=7e-76,
Organism=Escherichia coli, GI87082331, Length=105, Percent_Identity=36.1904761904762, Blast_Score=87, Evalue=4e-19,
Organism=Escherichia coli, GI87081747, Length=105, Percent_Identity=29.5238095238095, Blast_Score=69, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI212646193, Length=111, Percent_Identity=29.7297297297297, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507705, Length=75, Percent_Identity=42.6666666666667, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144092, Length=88, Percent_Identity=31.8181818181818, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17510299, Length=75, Percent_Identity=41.3333333333333, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17556044, Length=81, Percent_Identity=37.037037037037, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71998175, Length=71, Percent_Identity=40.8450704225352, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323072, Length=89, Percent_Identity=31.4606741573034, Blast_Score=62, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28573567, Length=93, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921434, Length=100, Percent_Identity=38, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24639826, Length=77, Percent_Identity=35.0649350649351, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005746
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR013766
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00085 Thioredoxin [H]

EC number: =1.8.1.8 [H]

Molecular weight: Translated: 15532; Mature: 15532

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVCTHCQAINRIPGDRLQDAAKCGRCGHELFDGEVINATGETLDKLLKDDLPVVIDFW
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHCCCCEEEECC
APWCGPCRNFAPIFEDVAEERSGKVRFVKVNTEAERELSARFGIRSIPMIMIFKHGQVVD
CCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCCEEEEECCCCHHH
MLNGAVPKAPFDSWLNEAL
HHCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MNTVCTHCQAINRIPGDRLQDAAKCGRCGHELFDGEVINATGETLDKLLKDDLPVVIDFW
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHCCCCEEEECC
APWCGPCRNFAPIFEDVAEERSGKVRFVKVNTEAERELSARFGIRSIPMIMIFKHGQVVD
CCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCCEEEEECCCCHHH
MLNGAVPKAPFDSWLNEAL
HHCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]