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The map label for this gene is ampE [H]

Identifier: 29140694

GI number: 29140694

Start: 173052

End: 173906

Strand: Direct

Name: ampE [H]

Synonym: t0153

Alternate gene names: 29140694

Gene position: 173052-173906 (Clockwise)

Preceding gene: 29140693

Following gene: 29140698

Centisome position: 3.61

GC content: 55.44

Gene sequence:

>855_bases
ATGACGCTGTTTACGACATTACTGGTGCTGATTGTCGAGCGCCTGTTTAAGCTGGGCGAGCACTGGCAGCTCGATCATCG
GCTGGAGGCGCTATTCCGTCGGATTACGCATTTTTCGATGTTGCGCACGATGGGAATGACGGCGATCGCGATGGTGGTGA
CTTTTCTACTGCTCCAGGCGTTAAAAGGGCTGCTATTTAACGTGCCGACGCTGGTGGTGTGGATTCTCCTGGGCGTGCTG
TGTATCGGCGCGGGCAAAGTGCGAGTACATTATCACGCCTGGCTGAAAGCGGCGTCCCGTAACGATCCGCATCCTTGCGA
GGCGATGGCCAGCGAGCTTACGCTGATTCACGGCGTGCCGCCGGATTGTAATGAGCGTGAGTTTTTGCGTGAGCTGCAAA
ACGCGTTGTTATGGATAAATTTCCGTTTTTACCTCGCACCGCTATTTTGGTTTATCGTTGGCGGTCCGTGGGGGCCGGTA
ACGCTGGTGGGCTATGCGTTTTTACGGGCCTGGCAGACCTGGCTGGCGCGCTATCAGACGCCGCACCAACGTTTGCAATC
CGGCATTGACGCTATTCTTCATGTACTGGACTGGATTCCGGTACGTCTGGCGGGCGTAGTGTATGCATTACTGGGGCATG
GCGAGAAAGCGTTGCCGGCCTGGTTTGCCTCGCTGGCGGATCTGCATACTTCGCAATATCAGGTGCTCACACGTCTGGCG
CAGTTCTCACTGGTGCGCGAACCGCATACTGACAAAGTAGAAACCCCCAAAGCGGCGGTATCGATGGCGAAGAAGGCCTC
GTTTGTGGTGGTGGTGATTATTGCGTTGCTCACCATTTATGGGGCATTAGTGTAA

Upstream 100 bases:

>100_bases
ACTGCAACATTGCGCCTGACCGCAAAACGGACCCTGGCCCTGCCTTTGACTGGCCGCGCTTTCGCGCTTTGGTAGCCCTC
TCGTCGCACAAGGAGATGAC

Downstream 100 bases:

>100_bases
TGTTGTGCCGATAGCGCTAACGCTTATGCTGCCTACAATGGAAAAATCTGTAGGCCCGGTAAGCGCTAGCGCCACCGGGC
AGTGCAACGGCTTAGATCGT

Product: regulatory protein AmpE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTLFTTLLVLIVERLFKLGEHWQLDHRLEALFRRITHFSMLRTMGMTAIAMVVTFLLLQALKGLLFNVPTLVVWILLGVL
CIGAGKVRVHYHAWLKAASRNDPHPCEAMASELTLIHGVPPDCNEREFLRELQNALLWINFRFYLAPLFWFIVGGPWGPV
TLVGYAFLRAWQTWLARYQTPHQRLQSGIDAILHVLDWIPVRLAGVVYALLGHGEKALPAWFASLADLHTSQYQVLTRLA
QFSLVREPHTDKVETPKAAVSMAKKASFVVVVIIALLTIYGALV

Sequences:

>Translated_284_residues
MTLFTTLLVLIVERLFKLGEHWQLDHRLEALFRRITHFSMLRTMGMTAIAMVVTFLLLQALKGLLFNVPTLVVWILLGVL
CIGAGKVRVHYHAWLKAASRNDPHPCEAMASELTLIHGVPPDCNEREFLRELQNALLWINFRFYLAPLFWFIVGGPWGPV
TLVGYAFLRAWQTWLARYQTPHQRLQSGIDAILHVLDWIPVRLAGVVYALLGHGEKALPAWFASLADLHTSQYQVLTRLA
QFSLVREPHTDKVETPKAAVSMAKKASFVVVVIIALLTIYGALV
>Mature_283_residues
TLFTTLLVLIVERLFKLGEHWQLDHRLEALFRRITHFSMLRTMGMTAIAMVVTFLLLQALKGLLFNVPTLVVWILLGVLC
IGAGKVRVHYHAWLKAASRNDPHPCEAMASELTLIHGVPPDCNEREFLRELQNALLWINFRFYLAPLFWFIVGGPWGPVT
LVGYAFLRAWQTWLARYQTPHQRLQSGIDAILHVLDWIPVRLAGVVYALLGHGEKALPAWFASLADLHTSQYQVLTRLAQ
FSLVREPHTDKVETPKAAVSMAKKASFVVVVIIALLTIYGALV

Specific function: Putative signaling protein in beta-lactamase regulation. AmpE seems not to act as a direct sensor for beta-lactams [H]

COG id: COG3725

COG function: function code V; Membrane protein required for beta-lactamase induction

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786301, Length=284, Percent_Identity=84.5070422535211, Blast_Score=481, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: NA

Molecular weight: Translated: 32153; Mature: 32021

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLFTTLLVLIVERLFKLGEHWQLDHRLEALFRRITHFSMLRTMGMTAIAMVVTFLLLQA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKGLLFNVPTLVVWILLGVLCIGAGKVRVHYHAWLKAASRNDPHPCEAMASELTLIHGVP
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
PDCNEREFLRELQNALLWINFRFYLAPLFWFIVGGPWGPVTLVGYAFLRAWQTWLARYQT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
PHQRLQSGIDAILHVLDWIPVRLAGVVYALLGHGEKALPAWFASLADLHTSQYQVLTRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QFSLVREPHTDKVETPKAAVSMAKKASFVVVVIIALLTIYGALV
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503 [H]