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The map label for this gene is ampD [H]

Identifier: 29140693

GI number: 29140693

Start: 172492

End: 173055

Strand: Direct

Name: ampD [H]

Synonym: t0152

Alternate gene names: 29140693

Gene position: 172492-173055 (Clockwise)

Preceding gene: 29140688

Following gene: 29140694

Centisome position: 3.6

GC content: 55.14

Gene sequence:

>564_bases
ATGTTGCCAGATAAGGGTTGGTTGGTAGAGGCGCGACGCGTCCCTTCTCCGCATTATGATTGCCGTCCGGATGACGAAAA
ACCGTCCTTGCTGGTGGTGCATAATATTAGCCTGCCCCCCGGCGAGTTTGGCGGTCCGTGGATCGATGCATTATTCACCG
GAACGATAGATCCAGACGCCCATCCTTTTTTTGCAGAAATCGCTCATCTTCGTGTTTCGGCCCATTGTCTGATTCGTCGC
GACGGTGAAATCGTCCAGTATGTGCCTTTTGATAAGCGTGCGTGGCATGCGGGCGTATCAAACTATCAGGGGCGGGAACG
TTGTAATGATTTCTCCATTGGTATTGAGCTGGAGGGGACGGATACGCTGGCTTATACCGATGCGCAGTATCAGCAGCTAG
CCGCCGTGACGCGCACGCTTATCGCTTGCTATCCGGCGATCGCCGACAATATGACCGGACACTGCAACATTGCGCCTGAC
CGCAAAACGGACCCTGGCCCTGCCTTTGACTGGCCGCGCTTTCGCGCTTTGGTAGCCCTCTCGTCGCACAAGGAGATGAC
ATGA

Upstream 100 bases:

>100_bases
GGCGAGGCGGCATGATAAAACTCCAAATTGCTAACGAATCATAAGGTAGAAACATGCTACTCTGAAGCAGCTATAAGCAC
CACTCAAAAGGAGACTCCGC

Downstream 100 bases:

>100_bases
CGCTGTTTACGACATTACTGGTGCTGATTGTCGAGCGCCTGTTTAAGCTGGGCGAGCACTGGCAGCTCGATCATCGGCTG
GAGGCGCTATTCCGTCGGAT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MLPDKGWLVEARRVPSPHYDCRPDDEKPSLLVVHNISLPPGEFGGPWIDALFTGTIDPDAHPFFAEIAHLRVSAHCLIRR
DGEIVQYVPFDKRAWHAGVSNYQGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRTLIACYPAIADNMTGHCNIAPD
RKTDPGPAFDWPRFRALVALSSHKEMT

Sequences:

>Translated_187_residues
MLPDKGWLVEARRVPSPHYDCRPDDEKPSLLVVHNISLPPGEFGGPWIDALFTGTIDPDAHPFFAEIAHLRVSAHCLIRR
DGEIVQYVPFDKRAWHAGVSNYQGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRTLIACYPAIADNMTGHCNIAPD
RKTDPGPAFDWPRFRALVALSSHKEMT
>Mature_187_residues
MLPDKGWLVEARRVPSPHYDCRPDDEKPSLLVVHNISLPPGEFGGPWIDALFTGTIDPDAHPFFAEIAHLRVSAHCLIRR
DGEIVQYVPFDKRAWHAGVSNYQGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRTLIACYPAIADNMTGHCNIAPD
RKTDPGPAFDWPRFRALVALSSHKEMT

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=179, Percent_Identity=91.0614525139665, Blast_Score=343, Evalue=3e-96,
Organism=Escherichia coli, GI1787092, Length=145, Percent_Identity=37.2413793103448, Blast_Score=80, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20929; Mature: 20929

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPDKGWLVEARRVPSPHYDCRPDDEKPSLLVVHNISLPPGEFGGPWIDALFTGTIDPDA
CCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEHEEECCCCCCC
HPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSNYQGRERCNDFSIGIELEGT
CHHHHHHHHHHHEEEEEEECCCCEEEEECCCCHHHHCCCCCCCCHHHCCCEEEEEEECCC
DTLAYTDAQYQQLAAVTRTLIACYPAIADNMTGHCNIAPDRKTDPGPAFDWPRFRALVAL
CEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHEEEE
SSHKEMT
CCCCCCC
>Mature Secondary Structure
MLPDKGWLVEARRVPSPHYDCRPDDEKPSLLVVHNISLPPGEFGGPWIDALFTGTIDPDA
CCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEHEEECCCCCCC
HPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSNYQGRERCNDFSIGIELEGT
CHHHHHHHHHHHEEEEEEECCCCEEEEECCCCHHHHCCCCCCCCHHHCCCEEEEEEECCC
DTLAYTDAQYQQLAAVTRTLIACYPAIADNMTGHCNIAPDRKTDPGPAFDWPRFRALVAL
CEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHEEEE
SSHKEMT
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 8419294 [H]