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The map label for this gene is rsrIM [H]

Identifier: 28572924

GI number: 28572924

Start: 887009

End: 887890

Strand: Direct

Name: rsrIM [H]

Synonym: TW783

Alternate gene names: 28572924

Gene position: 887009-887890 (Clockwise)

Preceding gene: 28572923

Following gene: 28572925

Centisome position: 95.8

GC content: 45.92

Gene sequence:

>882_bases
GTGCGGAACGGTGGTACCTGCTGGAGTCCAGACGGAGACAACCTTGTGGTGCAAGGTAATAACATTGACTTCCTTCACAA
ATTACCTGATGACTCCTTCCAACTCATATACATAGACCCACCATTCAACACGGGTAAACGACAAACCAGACAATGCAGCA
AGATGGTCAGATCACCCTACGGTGAACAGATCGGATTTTGCGGAAACAGGTACAAAATACTCTCGGAGAGTAGGACCGAA
TATGCAGATATGTTCGATGATTACCTGGGATTCCTAAAACCGCGACTGCACGAAGCATGGCGGCTCCTCAAGCCGGAAGG
GATGTTCTATCTACATCTTGATTACCGGGAGGTGCACTATGCAAAAATCCTACTGGATTCCATCTTTGGGCGCGATTCAT
TTATAAACGAGATCATTTGGGCGTATGACTACGGAGCAAAGACAAAAAGGAGATGGCCCGCAAAGCATGACAATATCCTC
GTATACGCCAAAGATCCACGAAAATATCTATTCGATAGCGAAAATGTTGATCGGGAGCCGTACATGGCCCCAGGGCTTGT
AACTCCTGATAAAGCGAAACGTGGAAAATTACCAACAGACGTTTGGTGGCACACAATAGTGTCACCAACCGGAAAAGAAA
AGACTGGGTATCCAACGCAAAAACCCGAGGGGGTTTTGAGGCGTATAATTCAAGCAAGCAGCCGTCCAAATGATTGGGTG
CTTGATTTTTTTGCTGGCAGCGGAACCACTGGGATTGTTGCGGGCAAATTGGGTCGTAAATTTGTTCTGATTGACAACAG
TCCTCAAGCCGTGAAAATAATGAAATTGAGAATAGATCGGAGTCTGTCAGAAAATCCAGTCAGGTACACAAAGCTGTATT
AA

Upstream 100 bases:

>100_bases
TAAGACAGTGGCACATAACGGGCATGCTCAATGGGTCTGTCAGATCAATCTTCGTATGTCTCACGCTGGGCTAAAGAAGC
GCCTCCACTAGAATGATCGG

Downstream 100 bases:

>100_bases
CAAAACCTGGATTATCTGGTGTTGGGCAGTGAATCTCACTTATACGTCGCTGGGCGCGCCTGTTGATCCCAGTCAAGTGT
CTCATAAGGGTCAATATGCG

Product: DNA methylase

Products: NA

Alternate protein names: M.RsrI; Adenine-specific methyltransferase RsrI [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MRNGGTCWSPDGDNLVVQGNNIDFLHKLPDDSFQLIYIDPPFNTGKRQTRQCSKMVRSPYGEQIGFCGNRYKILSESRTE
YADMFDDYLGFLKPRLHEAWRLLKPEGMFYLHLDYREVHYAKILLDSIFGRDSFINEIIWAYDYGAKTKRRWPAKHDNIL
VYAKDPRKYLFDSENVDREPYMAPGLVTPDKAKRGKLPTDVWWHTIVSPTGKEKTGYPTQKPEGVLRRIIQASSRPNDWV
LDFFAGSGTTGIVAGKLGRKFVLIDNSPQAVKIMKLRIDRSLSENPVRYTKLY

Sequences:

>Translated_293_residues
MRNGGTCWSPDGDNLVVQGNNIDFLHKLPDDSFQLIYIDPPFNTGKRQTRQCSKMVRSPYGEQIGFCGNRYKILSESRTE
YADMFDDYLGFLKPRLHEAWRLLKPEGMFYLHLDYREVHYAKILLDSIFGRDSFINEIIWAYDYGAKTKRRWPAKHDNIL
VYAKDPRKYLFDSENVDREPYMAPGLVTPDKAKRGKLPTDVWWHTIVSPTGKEKTGYPTQKPEGVLRRIIQASSRPNDWV
LDFFAGSGTTGIVAGKLGRKFVLIDNSPQAVKIMKLRIDRSLSENPVRYTKLY
>Mature_293_residues
MRNGGTCWSPDGDNLVVQGNNIDFLHKLPDDSFQLIYIDPPFNTGKRQTRQCSKMVRSPYGEQIGFCGNRYKILSESRTE
YADMFDDYLGFLKPRLHEAWRLLKPEGMFYLHLDYREVHYAKILLDSIFGRDSFINEIIWAYDYGAKTKRRWPAKHDNIL
VYAKDPRKYLFDSENVDREPYMAPGLVTPDKAKRGKLPTDVWWHTIVSPTGKEKTGYPTQKPEGVLRRIIQASSRPNDWV
LDFFAGSGTTGIVAGKLGRKFVLIDNSPQAVKIMKLRIDRSLSENPVRYTKLY

Specific function: This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-? on both strands, and protects the DNA from cleavage by the RsrI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=272, Percent_Identity=31.25, Blast_Score=110, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 33926; Mature: 33926

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNGGTCWSPDGDNLVVQGNNIDFLHKLPDDSFQLIYIDPPFNTGKRQTRQCSKMVRSPY
CCCCCCCCCCCCCEEEEECCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCC
GEQIGFCGNRYKILSESRTEYADMFDDYLGFLKPRLHEAWRLLKPEGMFYLHLDYREVHY
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHH
AKILLDSIFGRDSFINEIIWAYDYGAKTKRRWPAKHDNILVYAKDPRKYLFDSENVDREP
HHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCCC
YMAPGLVTPDKAKRGKLPTDVWWHTIVSPTGKEKTGYPTQKPEGVLRRIIQASSRPNDWV
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEE
LDFFAGSGTTGIVAGKLGRKFVLIDNSPQAVKIMKLRIDRSLSENPVRYTKLY
EEEECCCCCCEEEECCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCEEEECC
>Mature Secondary Structure
MRNGGTCWSPDGDNLVVQGNNIDFLHKLPDDSFQLIYIDPPFNTGKRQTRQCSKMVRSPY
CCCCCCCCCCCCCEEEEECCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCC
GEQIGFCGNRYKILSESRTEYADMFDDYLGFLKPRLHEAWRLLKPEGMFYLHLDYREVHY
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHH
AKILLDSIFGRDSFINEIIWAYDYGAKTKRRWPAKHDNILVYAKDPRKYLFDSENVDREP
HHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCCC
YMAPGLVTPDKAKRGKLPTDVWWHTIVSPTGKEKTGYPTQKPEGVLRRIIQASSRPNDWV
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEE
LDFFAGSGTTGIVAGKLGRKFVLIDNSPQAVKIMKLRIDRSLSENPVRYTKLY
EEEECCCCCCEEEECCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2690017; 2602165; 11024175 [H]