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The map label for this gene is gph [C]

Identifier: 28572923

GI number: 28572923

Start: 885973

End: 886731

Strand: Direct

Name: gph [C]

Synonym: TW782

Alternate gene names: 28572923

Gene position: 885973-886731 (Clockwise)

Preceding gene: 28572919

Following gene: 28572924

Centisome position: 95.68

GC content: 49.28

Gene sequence:

>759_bases
GTGCATGCGGATTTCTTATCGATTCATGCCGTCTCTGGGGCCGTCGAACTTACGGGCCCTATTGAAAGTGGTGAACATAA
GAGACAGGTGCAGAATACGGTGATGCGGAGATACAGTTGTGTATTATTTGATCTCGATGGGACGCTAATTGACTCTTTAG
AGGGCATAGTGAGCTCATTGAAGCGTGCAATTGAGTTCTTTGGCCTGCCGGTTCCCGATGACAGAACCATGCGGTCGTTT
GTTGGTCCGCCTTTGATTGAGAGTTTCACAAATATCTTGGGGCTATGCCGTAAAGATGCAGAAAAAGCCGTTGAGGTGTA
TCGCGAAAATTTTGATATCGGGGTTGCGTGCAATCCGCTATTCCCGGGTATCTGCGAGATGTTAGATAGCTTGCGAGAAG
CCAAGATGCCAATTGCGGTCGCAACATCCAAACCCAAACTCCTGGCCGTTCGGATACTTGACAACCATCAAATAACTCCT
TACTTCTGTGCCATAGAAGGCGGCAAGCCAAATGAAGAGGATGGAGAAAAGGCGATTGTTGTTGGGAATGCGCTAGGAGC
CCTGAATAATATGTGCGTTGACACCAGTCGGGCTGTTATGGTTGGCGACAGATGCTATGACTCCCACGGTGCTAGGGCAA
ACGGCTTACCCGCGATACTGGTCTCGTGGGGGTACGCGAGCAAAGAGGAATTGGCAAAGGAAAAGTTTGTCGTGGATGAT
GTAGAACAGCTAACTTCCGCCTTGCTTAGCCCCCTTTAA

Upstream 100 bases:

>100_bases
ATTTTTCATGGGATAAGTGGCGGCGCATACACTGGGCCGCGTGCTAGAATTTCGCAGGTATTTGGGTTACGAGTCTGGCG
TGACCGGCAATTTTAAGGGT

Downstream 100 bases:

>100_bases
GGCCCTGCACTAAGCTGGTCTGACTTTCACCTTACCAATCTGCAGTAAACTCGATTGCGGGTGCAATGGAGGATTCGCCT
AGTGGCCTATGGCGCGCGCT

Product: hydrolase

Products: Glycolate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MHADFLSIHAVSGAVELTGPIESGEHKRQVQNTVMRRYSCVLFDLDGTLIDSLEGIVSSLKRAIEFFGLPVPDDRTMRSF
VGPPLIESFTNILGLCRKDAEKAVEVYRENFDIGVACNPLFPGICEMLDSLREAKMPIAVATSKPKLLAVRILDNHQITP
YFCAIEGGKPNEEDGEKAIVVGNALGALNNMCVDTSRAVMVGDRCYDSHGARANGLPAILVSWGYASKEELAKEKFVVDD
VEQLTSALLSPL

Sequences:

>Translated_252_residues
MHADFLSIHAVSGAVELTGPIESGEHKRQVQNTVMRRYSCVLFDLDGTLIDSLEGIVSSLKRAIEFFGLPVPDDRTMRSF
VGPPLIESFTNILGLCRKDAEKAVEVYRENFDIGVACNPLFPGICEMLDSLREAKMPIAVATSKPKLLAVRILDNHQITP
YFCAIEGGKPNEEDGEKAIVVGNALGALNNMCVDTSRAVMVGDRCYDSHGARANGLPAILVSWGYASKEELAKEKFVVDD
VEQLTSALLSPL
>Mature_252_residues
MHADFLSIHAVSGAVELTGPIESGEHKRQVQNTVMRRYSCVLFDLDGTLIDSLEGIVSSLKRAIEFFGLPVPDDRTMRSF
VGPPLIESFTNILGLCRKDAEKAVEVYRENFDIGVACNPLFPGICEMLDSLREAKMPIAVATSKPKLLAVRILDNHQITP
YFCAIEGGKPNEEDGEKAIVVGNALGALNNMCVDTSRAVMVGDRCYDSHGARANGLPAILVSWGYASKEELAKEKFVVDD
VEQLTSALLSPL

Specific function: Unknown

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=236, Percent_Identity=24.5762711864407, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.18 [C]

Molecular weight: Translated: 27456; Mature: 27456

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHADFLSIHAVSGAVELTGPIESGEHKRQVQNTVMRRYSCVLFDLDGTLIDSLEGIVSSL
CCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHH
KRAIEFFGLPVPDDRTMRSFVGPPLIESFTNILGLCRKDAEKAVEVYRENFDIGVACNPL
HHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
FPGICEMLDSLREAKMPIAVATSKPKLLAVRILDNHQITPYFCAIEGGKPNEEDGEKAIV
CHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCCEEEE
VGNALGALNNMCVDTSRAVMVGDRCYDSHGARANGLPAILVSWGYASKEELAKEKFVVDD
EECHHHHHHHHHCCCCCEEEECCHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
VEQLTSALLSPL
HHHHHHHHHCCC
>Mature Secondary Structure
MHADFLSIHAVSGAVELTGPIESGEHKRQVQNTVMRRYSCVLFDLDGTLIDSLEGIVSSL
CCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHH
KRAIEFFGLPVPDDRTMRSFVGPPLIESFTNILGLCRKDAEKAVEVYRENFDIGVACNPL
HHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
FPGICEMLDSLREAKMPIAVATSKPKLLAVRILDNHQITPYFCAIEGGKPNEEDGEKAIV
CHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCCEEEE
VGNALGALNNMCVDTSRAVMVGDRCYDSHGARANGLPAILVSWGYASKEELAKEKFVVDD
EECHHHHHHHHHCCCCCEEEECCHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
VEQLTSALLSPL
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.57 {phosphoglycolate}} [C]

Substrates: D-Glycerate 2-phosphatelyc; H2O [C]

Specific reaction: D-Glycerate 2-phosphatelyc + H2O --> Glycolate + Phosphate [C]

General reaction: Additional information:

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12368430; 12218036 [H]