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The map label for this gene is metG [H]

Identifier: 28572916

GI number: 28572916

Start: 872789

End: 874540

Strand: Direct

Name: metG [H]

Synonym: TW775

Alternate gene names: 28572916

Gene position: 872789-874540 (Clockwise)

Preceding gene: 28572912

Following gene: 28572919

Centisome position: 94.26

GC content: 45.55

Gene sequence:

>1752_bases
ATGGGTTTTTATATTACAACCCCGGTGTTTTATGTAAATGATATCCCCCACATAGGTCATGCGTATACATTAGTCGTTTC
CGACGTTTTCGCGCGATGGCAACGACAGAGCGGAAAAGACACATTTTTTCTCTCAGGGACAGATGAGCACGGATTGAAAG
TGCTGCGTGCTGCAGAAGAGAAGAACATGACCCCACAGGGATGGGCTGACCTGATGGTGAAAACTGCCTGGAAGCCACTC
CTCGACCTAATGCACATTTCAAATGATGATTTTGTTCGCACAACGGAATCAAGGCATCGGGAGCGTGTTAAATGGTTTAT
AAATAGACTCAGAGAAAATCAATACATCTACAGCGGACAGTACAGCGGATACTATTGTGTCAGTTGCGAAGAATTCAAAA
CCGTTTCACAAAGTGCGCTGCCTAATGATGAGTCAGTTAATGGTACGCTCGGCAAAGACCCTCGTACATGCAGCCTGCCG
CAGGGTGAAGAACAAAATTATTTCTGCGAAGTGCACGGGTGCCAGCTCGAGCACTTCACCGAAAAGAACTATTTCTTTAG
GTTGAGCGCATTTACAGACAGATTACTTGATCTTTATAAGAAAAATCCGGACTTTATAGAACCCAAGTCTGCATATAACG
AAGTAGTACAGTTTGTAAAGAATGGAGTATCTGACCTCTCGATATCCAGGTCAAGTTTTACTTGGGGCATTGAGGTGCCC
TGGGATGAGGGGCATGTGGTCTATGTTTGGTTCGACGCATTGATTAACTATCTGACACCCGTAATGTCTGCTAAGGAAGA
GCTTCCGATCGATGGTACCACCCCTGGGGGTGCAGAATTTTGCACACACTCCAATCTGAATACACTTGAATCAAGTATTC
CAGGACCTGGAGGATCAGCAGAAAGCGATAGGCTATCCCATACTTTAAGTACCAGTGCCGACAATGCAGCTTTGACCTCT
CCGTTCAAAAAATACTGGCCAGCTAGCCATATAATCGGTAAAGACATATTGAGATTCCATGCCGTCATATGGCCTGCTAT
GCTGATGGCCGCAGGACTGCCTGTGCCGGAGAAAATATTTGCTCATGGTTGGCTGTTAGTTGACGGGCAGAAGATGTCAA
AGTCAAAGCTGACAGGTATTAAGCCGCAAGATTTAACAGATATTTTTGGCGTTGACGCAGTGAGATACTATTTTTTGAGA
CAGCCTGTTTTTGGGCAGGATTATTCATTCAGCATTGAGAGTATTGCAGCCTGCTACAACTCGGAGCTAGCAGATGGCTA
CGGGAATCTTGCCTGTCGACTAAGTTCCATGTACACCACATATCTGAATGGGCGCGTTATCGTGCCAAAGATTTATTCTG
AAGAAGACGAATCTTTGCTGCAGGCTTGTAAAGATGTGTCAGATAGGGCCTGTAAAGCAATAGATGAACTCATTCCACAC
ATCGCTATGGCTGAAATCTGGTCTCTTGCAGTCCGTGCAAATACATATACAACCAGGCAACAACCCTGGAGTTTACACAA
AAAGCAGGATGATGAGCGCCTAGCTTGCGTTCTTTACACAGCCCTCCGGGCTCTTTCAACTATCACAATTTTACTCTCCC
CCATCATGCCTCGGATAACGAACAAGCTATGGAGTGCATTTGGAGCAGATGGCGGTGTTAGTCAGCAAAATATACGCTCC
GCCTGGGGCGGGAAATTGTCAGACAAGGTAAGTCTGATACCTCCTATGTTTCCAAAAATCACCATCTCATAG

Upstream 100 bases:

>100_bases
GGGACTGACCATATCTCGCAAGATTCGAAAGTAAATTATCCGAACTAGACCGACAGTGCTATCCTGCCTGTAGGGCTGTG
CAATCCGGATATGGGGGCTT

Downstream 100 bases:

>100_bases
ACGTTCATATACGTATTCGTATAACAGACACTAATACATATACATGGTCTCTCTATGACTCTGGTAGCACTGAGGGCACA
GGTATCATTCCTGCAGATAA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MGFYITTPVFYVNDIPHIGHAYTLVVSDVFARWQRQSGKDTFFLSGTDEHGLKVLRAAEEKNMTPQGWADLMVKTAWKPL
LDLMHISNDDFVRTTESRHRERVKWFINRLRENQYIYSGQYSGYYCVSCEEFKTVSQSALPNDESVNGTLGKDPRTCSLP
QGEEQNYFCEVHGCQLEHFTEKNYFFRLSAFTDRLLDLYKKNPDFIEPKSAYNEVVQFVKNGVSDLSISRSSFTWGIEVP
WDEGHVVYVWFDALINYLTPVMSAKEELPIDGTTPGGAEFCTHSNLNTLESSIPGPGGSAESDRLSHTLSTSADNAALTS
PFKKYWPASHIIGKDILRFHAVIWPAMLMAAGLPVPEKIFAHGWLLVDGQKMSKSKLTGIKPQDLTDIFGVDAVRYYFLR
QPVFGQDYSFSIESIAACYNSELADGYGNLACRLSSMYTTYLNGRVIVPKIYSEEDESLLQACKDVSDRACKAIDELIPH
IAMAEIWSLAVRANTYTTRQQPWSLHKKQDDERLACVLYTALRALSTITILLSPIMPRITNKLWSAFGADGGVSQQNIRS
AWGGKLSDKVSLIPPMFPKITIS

Sequences:

>Translated_583_residues
MGFYITTPVFYVNDIPHIGHAYTLVVSDVFARWQRQSGKDTFFLSGTDEHGLKVLRAAEEKNMTPQGWADLMVKTAWKPL
LDLMHISNDDFVRTTESRHRERVKWFINRLRENQYIYSGQYSGYYCVSCEEFKTVSQSALPNDESVNGTLGKDPRTCSLP
QGEEQNYFCEVHGCQLEHFTEKNYFFRLSAFTDRLLDLYKKNPDFIEPKSAYNEVVQFVKNGVSDLSISRSSFTWGIEVP
WDEGHVVYVWFDALINYLTPVMSAKEELPIDGTTPGGAEFCTHSNLNTLESSIPGPGGSAESDRLSHTLSTSADNAALTS
PFKKYWPASHIIGKDILRFHAVIWPAMLMAAGLPVPEKIFAHGWLLVDGQKMSKSKLTGIKPQDLTDIFGVDAVRYYFLR
QPVFGQDYSFSIESIAACYNSELADGYGNLACRLSSMYTTYLNGRVIVPKIYSEEDESLLQACKDVSDRACKAIDELIPH
IAMAEIWSLAVRANTYTTRQQPWSLHKKQDDERLACVLYTALRALSTITILLSPIMPRITNKLWSAFGADGGVSQQNIRS
AWGGKLSDKVSLIPPMFPKITIS
>Mature_582_residues
GFYITTPVFYVNDIPHIGHAYTLVVSDVFARWQRQSGKDTFFLSGTDEHGLKVLRAAEEKNMTPQGWADLMVKTAWKPLL
DLMHISNDDFVRTTESRHRERVKWFINRLRENQYIYSGQYSGYYCVSCEEFKTVSQSALPNDESVNGTLGKDPRTCSLPQ
GEEQNYFCEVHGCQLEHFTEKNYFFRLSAFTDRLLDLYKKNPDFIEPKSAYNEVVQFVKNGVSDLSISRSSFTWGIEVPW
DEGHVVYVWFDALINYLTPVMSAKEELPIDGTTPGGAEFCTHSNLNTLESSIPGPGGSAESDRLSHTLSTSADNAALTSP
FKKYWPASHIIGKDILRFHAVIWPAMLMAAGLPVPEKIFAHGWLLVDGQKMSKSKLTGIKPQDLTDIFGVDAVRYYFLRQ
PVFGQDYSFSIESIAACYNSELADGYGNLACRLSSMYTTYLNGRVIVPKIYSEEDESLLQACKDVSDRACKAIDELIPHI
AMAEIWSLAVRANTYTTRQQPWSLHKKQDDERLACVLYTALRALSTITILLSPIMPRITNKLWSAFGADGGVSQQNIRSA
WGGKLSDKVSLIPPMFPKITIS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=570, Percent_Identity=30.7017543859649, Blast_Score=229, Evalue=7e-60,
Organism=Escherichia coli, GI1788432, Length=568, Percent_Identity=25, Blast_Score=155, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI212645096, Length=266, Percent_Identity=33.4586466165414, Blast_Score=145, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17540794, Length=574, Percent_Identity=25.7839721254355, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI71992781, Length=251, Percent_Identity=30.2788844621514, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321610, Length=564, Percent_Identity=31.3829787234043, Blast_Score=240, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6321704, Length=270, Percent_Identity=27.4074074074074, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21358483, Length=558, Percent_Identity=31.7204301075269, Blast_Score=240, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24655311, Length=245, Percent_Identity=27.3469387755102, Blast_Score=81, Evalue=3e-15,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 65701; Mature: 65570

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFYITTPVFYVNDIPHIGHAYTLVVSDVFARWQRQSGKDTFFLSGTDEHGLKVLRAAEE
CCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
KNMTPQGWADLMVKTAWKPLLDLMHISNDDFVRTTESRHRERVKWFINRLRENQYIYSGQ
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHCCCEEEECC
YSGYYCVSCEEFKTVSQSALPNDESVNGTLGKDPRTCSLPQGEEQNYFCEVHGCQLEHFT
CCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHCC
EKNYFFRLSAFTDRLLDLYKKNPDFIEPKSAYNEVVQFVKNGVSDLSISRSSFTWGIEVP
CCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHCEECCCCEEEEEECC
WDEGHVVYVWFDALINYLTPVMSAKEELPIDGTTPGGAEFCTHSNLNTLESSIPGPGGSA
CCCCCEEEEEHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHCCCCCHHHHHCCCCCCCCC
ESDRLSHTLSTSADNAALTSPFKKYWPASHIIGKDILRFHAVIWPAMLMAAGLPVPEKIF
CHHHHHHHHCCCCCCCHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AHGWLLVDGQKMSKSKLTGIKPQDLTDIFGVDAVRYYFLRQPVFGQDYSFSIESIAACYN
HCCEEEEECCHHCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHC
SELADGYGNLACRLSSMYTTYLNGRVIVPKIYSEEDESLLQACKDVSDRACKAIDELIPH
CHHHCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAMAEIWSLAVRANTYTTRQQPWSLHKKQDDERLACVLYTALRALSTITILLSPIMPRIT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKLWSAFGADGGVSQQNIRSAWGGKLSDKVSLIPPMFPKITIS
HHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEC
>Mature Secondary Structure 
GFYITTPVFYVNDIPHIGHAYTLVVSDVFARWQRQSGKDTFFLSGTDEHGLKVLRAAEE
CEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
KNMTPQGWADLMVKTAWKPLLDLMHISNDDFVRTTESRHRERVKWFINRLRENQYIYSGQ
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHCCCEEEECC
YSGYYCVSCEEFKTVSQSALPNDESVNGTLGKDPRTCSLPQGEEQNYFCEVHGCQLEHFT
CCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHCC
EKNYFFRLSAFTDRLLDLYKKNPDFIEPKSAYNEVVQFVKNGVSDLSISRSSFTWGIEVP
CCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHCEECCCCEEEEEECC
WDEGHVVYVWFDALINYLTPVMSAKEELPIDGTTPGGAEFCTHSNLNTLESSIPGPGGSA
CCCCCEEEEEHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHCCCCCHHHHHCCCCCCCCC
ESDRLSHTLSTSADNAALTSPFKKYWPASHIIGKDILRFHAVIWPAMLMAAGLPVPEKIF
CHHHHHHHHCCCCCCCHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AHGWLLVDGQKMSKSKLTGIKPQDLTDIFGVDAVRYYFLRQPVFGQDYSFSIESIAACYN
HCCEEEEECCHHCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHC
SELADGYGNLACRLSSMYTTYLNGRVIVPKIYSEEDESLLQACKDVSDRACKAIDELIPH
CHHHCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAMAEIWSLAVRANTYTTRQQPWSLHKKQDDERLACVLYTALRALSTITILLSPIMPRIT
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKLWSAFGADGGVSQQNIRSAWGGKLSDKVSLIPPMFPKITIS
HHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]