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The map label for this gene is ddlA

Identifier: 28572416

GI number: 28572416

Start: 286979

End: 288022

Strand: Direct

Name: ddlA

Synonym: TW257

Alternate gene names: 28572416

Gene position: 286979-288022 (Clockwise)

Preceding gene: 28572415

Following gene: 28572422

Centisome position: 30.99

GC content: 43.97

Gene sequence:

>1044_bases
ATGCGCAGCTTGCTCTTATTATTTGGTGGTCGCTCACAAGAGCATTCGGTTAGTTATGCATCGGCCAGGGCGTTGCTTGA
ACATATATGCGATGAATGGACTGTAATTCCTGTTGGCATAACAAAACACGGCGATTTTGTATACTGCAGCACTGTGTCAG
AGCAGGATTATGGCGAAGTCATTGATAATGGTAATAGAGTTGTTTGGCCCAGCCGCGCGGGGTGCAAAAAAATTGAGGTA
GTCCAGGCTCGCGGCAAAAGCTTTTTTGTTGAGTTCGATCTCGTATTTCCTCTCCTTCATGGGGTGTTTGGTGAAGACGG
AACAATCCAGGGGATGCTTGACCTGCTTGATATTCCTTATGTTGGTTCCGGAGTATTTGCAAGTGCAGCTGCGATGGATA
AGCACTATACAAAAATACTGTGTTCCCACGCGGGCATTCCGGTTCTGCCGTGGTATCTCATTAAGGGGCATGCTTGGCAT
AAGAACAGAGATTCTTTTTTGAAGCAAATTTCTGACAGATTTACCTTTCCTCTGTTTGTGAAGCCTGTTGATGCTGGCTC
GAGTTTTGGTTGCACTTTTGTTGATTTTTTCGAGCAATTGCCAGTGGCTATAGAACATGCTCTTCAACACGGAAAATCTG
CTATTGTTGAGCCTGCCCTTGATGCTCCAGAAGTTTTTTGCTCACTCATTGAAAATAATGGGGTATTGCAGTGCTCTGAA
CTCGTTTTTGTAAAACATAGTGGCAGTAAGTTTTATGATTATTCCGCCAAATACCTGAACCCGGTTGAGTTTGTGATTCC
AGCTCAGTTTGACAATACCGACGGATATGCCGAGATTGCTTCAAAAGTTTTTTTCGAGCTTGGCTGTCGCCACTACGCTC
GGATTGATTTTTTTGTTACAGAATCGGGTCTTGTTCTAAATGAGATTAACACATCACCCGGCCTCACTCAGAAATCGATT
TTTCCATCAAGCTTTAAGTCGATGCAATACAGTCAAGTTATAGAGACTATTCTTGCCTCAGCCTACTGCCAGGTCAAACG
CTAA

Upstream 100 bases:

>100_bases
GCAGTGGTGTATACATGCCAATTGCGCAAGCGTTAAGTCAGGTTATTAGCGGTAAACGAACGGCACATTCGCTTCTGGAT
ATTTGTTTTAGCCTGGCGTA

Downstream 100 bases:

>100_bases
ACTGAGACTTGCATCAACTGCATGAAGCCACTTTTGGCAGGTAATTTACCGCCAACCCAAGTGTTGCCAGTGCTGTTGCT
GGTGATATTTTAAAATTGTC

Product: D-alanine--D-alanine ligase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MRSLLLLFGGRSQEHSVSYASARALLEHICDEWTVIPVGITKHGDFVYCSTVSEQDYGEVIDNGNRVVWPSRAGCKKIEV
VQARGKSFFVEFDLVFPLLHGVFGEDGTIQGMLDLLDIPYVGSGVFASAAAMDKHYTKILCSHAGIPVLPWYLIKGHAWH
KNRDSFLKQISDRFTFPLFVKPVDAGSSFGCTFVDFFEQLPVAIEHALQHGKSAIVEPALDAPEVFCSLIENNGVLQCSE
LVFVKHSGSKFYDYSAKYLNPVEFVIPAQFDNTDGYAEIASKVFFELGCRHYARIDFFVTESGLVLNEINTSPGLTQKSI
FPSSFKSMQYSQVIETILASAYCQVKR

Sequences:

>Translated_347_residues
MRSLLLLFGGRSQEHSVSYASARALLEHICDEWTVIPVGITKHGDFVYCSTVSEQDYGEVIDNGNRVVWPSRAGCKKIEV
VQARGKSFFVEFDLVFPLLHGVFGEDGTIQGMLDLLDIPYVGSGVFASAAAMDKHYTKILCSHAGIPVLPWYLIKGHAWH
KNRDSFLKQISDRFTFPLFVKPVDAGSSFGCTFVDFFEQLPVAIEHALQHGKSAIVEPALDAPEVFCSLIENNGVLQCSE
LVFVKHSGSKFYDYSAKYLNPVEFVIPAQFDNTDGYAEIASKVFFELGCRHYARIDFFVTESGLVLNEINTSPGLTQKSI
FPSSFKSMQYSQVIETILASAYCQVKR
>Mature_347_residues
MRSLLLLFGGRSQEHSVSYASARALLEHICDEWTVIPVGITKHGDFVYCSTVSEQDYGEVIDNGNRVVWPSRAGCKKIEV
VQARGKSFFVEFDLVFPLLHGVFGEDGTIQGMLDLLDIPYVGSGVFASAAAMDKHYTKILCSHAGIPVLPWYLIKGHAWH
KNRDSFLKQISDRFTFPLFVKPVDAGSSFGCTFVDFFEQLPVAIEHALQHGKSAIVEPALDAPEVFCSLIENNGVLQCSE
LVFVKHSGSKFYDYSAKYLNPVEFVIPAQFDNTDGYAEIASKVFFELGCRHYARIDFFVTESGLVLNEINTSPGLTQKSI
FPSSFKSMQYSQVIETILASAYCQVKR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=353, Percent_Identity=32.2946175637394, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1786280, Length=270, Percent_Identity=37.037037037037, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_TROW8 (Q83I36)

Other databases:

- EMBL:   BX251410
- RefSeq:   NP_789196.1
- ProteinModelPortal:   Q83I36
- STRING:   Q83I36
- GeneID:   1064548
- GenomeReviews:   BX072543_GR
- KEGG:   tws:TW257
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   EVLVEKW
- PhylomeDB:   Q83I36
- ProtClustDB:   CLSK229604
- BioCyc:   TWHI218496:TW0241-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 38660; Mature: 38660

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSLLLLFGGRSQEHSVSYASARALLEHICDEWTVIPVGITKHGDFVYCSTVSEQDYGEV
CCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCHHHH
IDNGNRVVWPSRAGCKKIEVVQARGKSFFVEFDLVFPLLHGVFGEDGTIQGMLDLLDIPY
HCCCCEEEECCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
VGSGVFASAAAMDKHYTKILCSHAGIPVLPWYLIKGHAWHKNRDSFLKQISDRFTFPLFV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCHHHHHHHHHHCCCCEEEE
KPVDAGSSFGCTFVDFFEQLPVAIEHALQHGKSAIVEPALDAPEVFCSLIENNGVLQCSE
EEECCCCCCCCHHHHHHHHCCHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHCCCCEEEEE
LVFVKHSGSKFYDYSAKYLNPVEFVIPAQFDNTDGYAEIASKVFFELGCRHYARIDFFVT
EEEEEECCCEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEE
ESGLVLNEINTSPGLTQKSIFPSSFKSMQYSQVIETILASAYCQVKR
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSLLLLFGGRSQEHSVSYASARALLEHICDEWTVIPVGITKHGDFVYCSTVSEQDYGEV
CCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCHHHH
IDNGNRVVWPSRAGCKKIEVVQARGKSFFVEFDLVFPLLHGVFGEDGTIQGMLDLLDIPY
HCCCCEEEECCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
VGSGVFASAAAMDKHYTKILCSHAGIPVLPWYLIKGHAWHKNRDSFLKQISDRFTFPLFV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCHHHHHHHHHHCCCCEEEE
KPVDAGSSFGCTFVDFFEQLPVAIEHALQHGKSAIVEPALDAPEVFCSLIENNGVLQCSE
EEECCCCCCCCHHHHHHHHCCHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHCCCCEEEEE
LVFVKHSGSKFYDYSAKYLNPVEFVIPAQFDNTDGYAEIASKVFFELGCRHYARIDFFVT
EEEEEECCCEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEE
ESGLVLNEINTSPGLTQKSIFPSSFKSMQYSQVIETILASAYCQVKR
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12606174