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The map label for this gene is gpsA

Identifier: 28572415

GI number: 28572415

Start: 286002

End: 286979

Strand: Direct

Name: gpsA

Synonym: TW256

Alternate gene names: 28572415

Gene position: 286002-286979 (Clockwise)

Preceding gene: 28572414

Following gene: 28572416

Centisome position: 30.89

GC content: 46.93

Gene sequence:

>978_bases
ATGAAAGAGGGTGGGTTGCGCAATAAAGTCGCTGTTATAGGTTCTGGATCATGGGGGACCGCCATTGCCAATCTGCTTTG
TAAGGCGGGGAATGAAACTATTCTATGGGGCAGGGATGAAAATGTTATTGATGAGATAAATAACGCCAGAGTCAATTCGA
AATATCTTCCAGGAGTAGAATTATTCCTACGGGCAACTTGTGATCTTGATTATGCCGTTGCCGACGCCTCCCATGTATAC
ATAGCATTACCGAGCTTTGCGCTATCAAAAGTATTACCCAAATTATCCCTGGATAAGTTTTCCATTGTAATAAGTCTTAT
AAAGTGTCTTGAGCCTGACACGGGCAGACGGATGAGTGAGGTTATTTCGGAGGCTTTGGACCTCGGTCACAATCGCCTTG
CCGTCATAAGCGGCCCTAATCTCGCACTTGAAGTGGCCAATGACGAACCCAGTGTGTCGGTTGTTGCGTCTGCGAATATT
GCAACAGCGAATATTGTGGCTGGCACTCTGAAGTGTCCTGGGTTTTATTGCATTCCAAGTTCCGACATCAAAGGTGTTGA
AATATGTGCTGCCTCAAAAAATCTGGTTGCACTAATATCTGGAATAGCCAGAGGTATGGATCTTGGGGATAATACACGGG
CTGCGCTAATAACGCTCGGATTCAGAGAGCTCTTGCGTCTTGTGCTTGAAAATGGTGGTACTGAGGAAACTGTTTTTGGC
GTAGCGGGCCTGGGTGATGTGGTGGCAACGTGCAATTCTCATTTGTCAAGAAATAACAAAGCAGGAGTTTTGCTTGCAAA
GGGTGCCCCCCTTGATGAGGTAAAACAAACCGCAGAGGGGGTTGTTGCCATTTCGGGCGTTCTCGCCCTCGCGGAACGCA
GTGGTGTATACATGCCAATTGCGCAAGCGTTAAGTCAGGTTATTAGCGGTAAACGAACGGCACATTCGCTTCTGGATATT
TGTTTTAGCCTGGCGTAA

Upstream 100 bases:

>100_bases
TCAGATGAGGAGATTAAGCGCTTCAGTAACCTGATGAAGGAAAAAATTAATACAGCCCTTGCAGCTATCAGGTTATCCGA
TGAGACAGGCTTCATGCGAC

Downstream 100 bases:

>100_bases
TGCGCAGCTTGCTCTTATTATTTGGTGGTCGCTCACAAGAGCATTCGGTTAGTTATGCATCGGCCAGGGCGTTGCTTGAA
CATATATGCGATGAATGGAC

Product: glycerol-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKEGGLRNKVAVIGSGSWGTAIANLLCKAGNETILWGRDENVIDEINNARVNSKYLPGVELFLRATCDLDYAVADASHVY
IALPSFALSKVLPKLSLDKFSIVISLIKCLEPDTGRRMSEVISEALDLGHNRLAVISGPNLALEVANDEPSVSVVASANI
ATANIVAGTLKCPGFYCIPSSDIKGVEICAASKNLVALISGIARGMDLGDNTRAALITLGFRELLRLVLENGGTEETVFG
VAGLGDVVATCNSHLSRNNKAGVLLAKGAPLDEVKQTAEGVVAISGVLALAERSGVYMPIAQALSQVISGKRTAHSLLDI
CFSLA

Sequences:

>Translated_325_residues
MKEGGLRNKVAVIGSGSWGTAIANLLCKAGNETILWGRDENVIDEINNARVNSKYLPGVELFLRATCDLDYAVADASHVY
IALPSFALSKVLPKLSLDKFSIVISLIKCLEPDTGRRMSEVISEALDLGHNRLAVISGPNLALEVANDEPSVSVVASANI
ATANIVAGTLKCPGFYCIPSSDIKGVEICAASKNLVALISGIARGMDLGDNTRAALITLGFRELLRLVLENGGTEETVFG
VAGLGDVVATCNSHLSRNNKAGVLLAKGAPLDEVKQTAEGVVAISGVLALAERSGVYMPIAQALSQVISGKRTAHSLLDI
CFSLA
>Mature_325_residues
MKEGGLRNKVAVIGSGSWGTAIANLLCKAGNETILWGRDENVIDEINNARVNSKYLPGVELFLRATCDLDYAVADASHVY
IALPSFALSKVLPKLSLDKFSIVISLIKCLEPDTGRRMSEVISEALDLGHNRLAVISGPNLALEVANDEPSVSVVASANI
ATANIVAGTLKCPGFYCIPSSDIKGVEICAASKNLVALISGIARGMDLGDNTRAALITLGFRELLRLVLENGGTEETVFG
VAGLGDVVATCNSHLSRNNKAGVLLAKGAPLDEVKQTAEGVVAISGVLALAERSGVYMPIAQALSQVISGKRTAHSLLDI
CFSLA

Specific function: De novo phospholipid biosynthesis; glycerol-3 phosphate formation. [C]

COG id: COG0240

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI24307999, Length=340, Percent_Identity=27.3529411764706, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI33695088, Length=281, Percent_Identity=29.8932384341637, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1790037, Length=308, Percent_Identity=35.3896103896104, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17507425, Length=348, Percent_Identity=26.7241379310345, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI32564399, Length=343, Percent_Identity=29.1545189504373, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI193210136, Length=352, Percent_Identity=29.2613636363636, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32564403, Length=352, Percent_Identity=29.2613636363636, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI193210134, Length=337, Percent_Identity=28.1899109792285, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320181, Length=352, Percent_Identity=28.9772727272727, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6324513, Length=340, Percent_Identity=28.5294117647059, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI22026922, Length=291, Percent_Identity=29.8969072164948, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17136204, Length=348, Percent_Identity=26.7241379310345, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17136202, Length=348, Percent_Identity=26.7241379310345, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17136200, Length=348, Percent_Identity=26.7241379310345, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281362270, Length=291, Percent_Identity=25.4295532646048, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45551945, Length=291, Percent_Identity=25.4295532646048, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648969, Length=247, Percent_Identity=25.9109311740891, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPDA_TROWT (Q83G27)

Other databases:

- EMBL:   AE014184
- RefSeq:   NP_787634.1
- ProteinModelPortal:   Q83G27
- SMR:   Q83G27
- STRING:   Q83G27
- GeneID:   1482490
- GenomeReviews:   AE014184_GR
- KEGG:   twh:TWT506
- eggNOG:   COG0240
- HOGENOM:   HBG586392
- OMA:   SQTLRGN
- ProtClustDB:   CLSK229603
- BioCyc:   TWHI203267:TW506-MONOMER
- HAMAP:   MF_00394
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR006168
- InterPro:   IPR006109
- InterPro:   IPR011128
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR11728
- PIRSF:   PIRSF000114
- PRINTS:   PR00077

Pfam domain/function: PF07479 NAD_Gly3P_dh_C; PF01210 NAD_Gly3P_dh_N; SSF48179 6DGDH_C_like

EC number: =1.1.1.94

Molecular weight: Translated: 34013; Mature: 34013

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00957 NAD_G3PDH

Important sites: ACT_SITE 207-207 BINDING 122-122 BINDING 122-122 BINDING 156-156 BINDING 271-271 BINDING 293-293

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEGGLRNKVAVIGSGSWGTAIANLLCKAGNETILWGRDENVIDEINNARVNSKYLPGVE
CCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCCCCCCCHH
LFLRATCDLDYAVADASHVYIALPSFALSKVLPKLSLDKFSIVISLIKCLEPDTGRRMSE
HHHHHHCCCCEEECCCCEEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHH
VISEALDLGHNRLAVISGPNLALEVANDEPSVSVVASANIATANIVAGTLKCPGFYCIPS
HHHHHHHCCCCEEEEEECCCEEEEECCCCCCEEEEEECCCEEEHEEEEEECCCEEEEECC
SDIKGVEICAASKNLVALISGIARGMDLGDNTRAALITLGFRELLRLVLENGGTEETVFG
CCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCHHEEE
VAGLGDVVATCNSHLSRNNKAGVLLAKGAPLDEVKQTAEGVVAISGVLALAERSGVYMPI
CCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHH
AQALSQVISGKRTAHSLLDICFSLA
HHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEGGLRNKVAVIGSGSWGTAIANLLCKAGNETILWGRDENVIDEINNARVNSKYLPGVE
CCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCCCCCCCHH
LFLRATCDLDYAVADASHVYIALPSFALSKVLPKLSLDKFSIVISLIKCLEPDTGRRMSE
HHHHHHCCCCEEECCCCEEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHH
VISEALDLGHNRLAVISGPNLALEVANDEPSVSVVASANIATANIVAGTLKCPGFYCIPS
HHHHHHHCCCCEEEEEECCCEEEEECCCCCCEEEEEECCCEEEHEEEEEECCCEEEEECC
SDIKGVEICAASKNLVALISGIARGMDLGDNTRAALITLGFRELLRLVLENGGTEETVFG
CCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCHHEEE
VAGLGDVVATCNSHLSRNNKAGVLLAKGAPLDEVKQTAEGVVAISGVLALAERSGVYMPI
CCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHH
AQALSQVISGKRTAHSLLDICFSLA
HHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12902375