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The map label for this gene is hrdB [H]

Identifier: 28572422

GI number: 28572422

Start: 296144

End: 297307

Strand: Direct

Name: hrdB [H]

Synonym: TW263

Alternate gene names: 28572422

Gene position: 296144-297307 (Clockwise)

Preceding gene: 28572416

Following gene: 28572423

Centisome position: 31.98

GC content: 51.2

Gene sequence:

>1164_bases
ATGCCGGTAAAGATAAAAACCCAGTCGCGCGGGTCTGATAAAGAGGCTTTTGCCGGGGCTCCCTCTACTTCGGGTGCTTC
CGGGGCCGTTCTTGACGAGGATCTTTCAACTGATCCTGCTTCGCATATCTCTGCACAAGCCACTGCATCCGATACCGACG
TTGCATCTCAAGGATCGGAAGGGGATTTCGAGGCGATCGTCCTTGCGGATACAAAGGATGATGAGTCAACCCCGGCTTCG
CCCGTAATTTCTGGCGCCACTGCTGACCCTGTAAAAGATTATTTAAAGCAAATCGGTAAGGTATCACTCCTAACGGCCGA
CGAGGAGGTTAGCCTTGCGAAGCGGATCGAGGCGGGCCTGTTTGCCGAGGAAAAACTCTCAGAGTCAGGTGATCTCGCGC
CGAAGCTCAAGCGTGAGCTGAGGTGGGTTGTGCGCGACGGTCAGCGTGCGAAAGACCACCTTTTAGCCGCTAATTTGCGA
TTAGTTGTTTCTCTGGCCAAGCGGTATACAGGCCGAGGAATGCAGTTTCTTGACTTAATTCAAGAAGGTAATTTGGGTCT
CATTAGGGCTGTTGAGAAGTTTGACTATGTAAAGGGATTTAAGTTCTCAACTTATGCGACATGGTGGATTCGTCAGGCTA
TCACTCGGGCCATGGCCGATCAGGCACGGACAATCCGTATTCCCGTTCATATGGTCGAGGTTATAAACAAGCTTTCCAGA
GTGCAAAGGCAACTTCTACAGGATCTTGGACGTGAACCGACTCCGGATGAACTGGGTCGTGAGCTTGACATGCCACCGGA
GAGGGTCGTTGAGGTTCAGAAATACGGGCGTGAACCGATTTCTCTTCACACCCCTCTCGGCGATGAGGGTGACAGTGAGT
TTGGCGATTTGATAGAGGATGTTGACGCTGTTGCGCCTGACGATGCCGTTGGGTTTAGTATGCTTCAGCAGCAACTTCGT
CTTCTTCTTGATTCTCTTTCGGAGCGTGAAGCCGGCGTTATTCGTATGCGCTTTGGACTTGATTATGACATGCCCAAGAC
ATTGGACTACGTCGGTGAGTATTTTGATGTAACTCGGGAGCGCATTAGGCAGATTGAGGCAAAGACGATGGCGAAGTTAC
GGCACCCATCGCGCTCTCAATGTTTGAGGGATTATTTGGGGTAA

Upstream 100 bases:

>100_bases
GAATGAGAATCGTGTCTTGCATTCTGGGTGTGTGTTTCTGCACTACAGTAGTCTATATTCTCTCCGGAGGGGAGTCTCAT
GTTATAATTCCCCCTAGGTT

Downstream 100 bases:

>100_bases
CTTGAGGGGTTGCCTGGCCATCTTCTTCGGCCGCGCTCTTCTTGTTGCCTTGCGTGTTCTACGTTCTGGCGGGTCAGTCT
TACCCGGTGTTCTTGCTGAG

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MPVKIKTQSRGSDKEAFAGAPSTSGASGAVLDEDLSTDPASHISAQATASDTDVASQGSEGDFEAIVLADTKDDESTPAS
PVISGATADPVKDYLKQIGKVSLLTADEEVSLAKRIEAGLFAEEKLSESGDLAPKLKRELRWVVRDGQRAKDHLLAANLR
LVVSLAKRYTGRGMQFLDLIQEGNLGLIRAVEKFDYVKGFKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLSR
VQRQLLQDLGREPTPDELGRELDMPPERVVEVQKYGREPISLHTPLGDEGDSEFGDLIEDVDAVAPDDAVGFSMLQQQLR
LLLDSLSEREAGVIRMRFGLDYDMPKTLDYVGEYFDVTRERIRQIEAKTMAKLRHPSRSQCLRDYLG

Sequences:

>Translated_387_residues
MPVKIKTQSRGSDKEAFAGAPSTSGASGAVLDEDLSTDPASHISAQATASDTDVASQGSEGDFEAIVLADTKDDESTPAS
PVISGATADPVKDYLKQIGKVSLLTADEEVSLAKRIEAGLFAEEKLSESGDLAPKLKRELRWVVRDGQRAKDHLLAANLR
LVVSLAKRYTGRGMQFLDLIQEGNLGLIRAVEKFDYVKGFKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLSR
VQRQLLQDLGREPTPDELGRELDMPPERVVEVQKYGREPISLHTPLGDEGDSEFGDLIEDVDAVAPDDAVGFSMLQQQLR
LLLDSLSEREAGVIRMRFGLDYDMPKTLDYVGEYFDVTRERIRQIEAKTMAKLRHPSRSQCLRDYLG
>Mature_386_residues
PVKIKTQSRGSDKEAFAGAPSTSGASGAVLDEDLSTDPASHISAQATASDTDVASQGSEGDFEAIVLADTKDDESTPASP
VISGATADPVKDYLKQIGKVSLLTADEEVSLAKRIEAGLFAEEKLSESGDLAPKLKRELRWVVRDGQRAKDHLLAANLRL
VVSLAKRYTGRGMQFLDLIQEGNLGLIRAVEKFDYVKGFKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLSRV
QRQLLQDLGREPTPDELGRELDMPPERVVEVQKYGREPISLHTPLGDEGDSEFGDLIEDVDAVAPDDAVGFSMLQQQLRL
LLDSLSEREAGVIRMRFGLDYDMPKTLDYVGEYFDVTRERIRQIEAKTMAKLRHPSRSQCLRDYLG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=240, Percent_Identity=60, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1789098, Length=324, Percent_Identity=37.3456790123457, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1789871, Length=299, Percent_Identity=28.7625418060201, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 42895; Mature: 42764

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVKIKTQSRGSDKEAFAGAPSTSGASGAVLDEDLSTDPASHISAQATASDTDVASQGSE
CCCEECCCCCCCCCHHHCCCCCCCCCCCCEECCCCCCCCHHHHCCCCCCCCCHHHCCCCC
GDFEAIVLADTKDDESTPASPVISGATADPVKDYLKQIGKVSLLTADEEVSLAKRIEAGL
CCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCC
FAEEKLSESGDLAPKLKRELRWVVRDGQRAKDHLLAANLRLVVSLAKRYTGRGMQFLDLI
CHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
QEGNLGLIRAVEKFDYVKGFKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLSR
HCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHH
VQRQLLQDLGREPTPDELGRELDMPPERVVEVQKYGREPISLHTPLGDEGDSEFGDLIED
HHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHH
VDAVAPDDAVGFSMLQQQLRLLLDSLSEREAGVIRMRFGLDYDMPKTLDYVGEYFDVTRE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
RIRQIEAKTMAKLRHPSRSQCLRDYLG
HHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure 
PVKIKTQSRGSDKEAFAGAPSTSGASGAVLDEDLSTDPASHISAQATASDTDVASQGSE
CCEECCCCCCCCCHHHCCCCCCCCCCCCEECCCCCCCCHHHHCCCCCCCCCHHHCCCCC
GDFEAIVLADTKDDESTPASPVISGATADPVKDYLKQIGKVSLLTADEEVSLAKRIEAGL
CCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCC
FAEEKLSESGDLAPKLKRELRWVVRDGQRAKDHLLAANLRLVVSLAKRYTGRGMQFLDLI
CHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
QEGNLGLIRAVEKFDYVKGFKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLSR
HCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHH
VQRQLLQDLGREPTPDELGRELDMPPERVVEVQKYGREPISLHTPLGDEGDSEFGDLIED
HHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHH
VDAVAPDDAVGFSMLQQQLRLLLDSLSEREAGVIRMRFGLDYDMPKTLDYVGEYFDVTRE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
RIRQIEAKTMAKLRHPSRSQCLRDYLG
HHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8690707 [H]