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The map label for this gene is hpk3 [H]

Identifier: 28377604

GI number: 28377604

Start: 681237

End: 682640

Strand: Direct

Name: hpk3 [H]

Synonym: lp_0744

Alternate gene names: 28377604

Gene position: 681237-682640 (Clockwise)

Preceding gene: 28377603

Following gene: 28377605

Centisome position: 20.59

GC content: 46.58

Gene sequence:

>1404_bases
ATGCATAATCGCTGGCAACAGACGACACGGTTATTGCTCATGGAAAATGTGTTACTGGTTCTGATCGTGTTGGTCGTGTT
GCAGCACTTTTCGCCAATTAAGTGGTCCTGGCGCCTAGTCGGTTGTTTAGTTGGTGCTTGGCTGGTTTTGGCAGTAATCG
AGGGCTTGATCGCTTGGCGGCAACAAAGTGAACGGCAGCGCACGATCGACCGTATGGAAGCTAAGCTTCGTCAGATGAGT
AGCCAGCAATTTCCACCACATATCTTATTGCCACAATCGGACCCCTTATACAGCCTAGCACAAGCGGTCAACCAGTTAGA
GAGTTATCAAAAAAGTCAGACGCGCCGTGCGGAACTGCAAGAAAAAGAGTTAATGATGATTATGCGGTACTTGCCTGTGG
GAGTGATGGTCATTGACCACCGGCGACAGGTTCAACTGTCTAATCCGGCGATGAGTGAACTATTGCAAGTCTCGGTTAAT
ACGGTGGCCCACATTTATACCAAGGATATTCAGCTGTACGCGTTGACTAAATTGATTGAAGATACGATTACTAGTGGCAA
AAATCAACGGGCAACGATTACGCTGATCCCAGCGCCTAACACGCAGGTTGTCGAGGCTTCAACCGTTTATATCCAAGGCA
ATCGTTCACAATTTCAAATTGTCCTGATGTTGTATGATATCACTGAAGTCTATATGATTGAACAAATGCAGGATGATTTT
GTCAGCAACGCTAGCCACGAGTTGAAGACGCCGATTACAGCCATTTCCGGTTTCACTGAAACGTTGTTAAGTGGTGCCAA
GGATGATCCGGACACGCTTGACCAATTCTTGCATATTATTGCGGATGAAAGTCAACGGTTGATTGATCTCATTCAGGATG
TTCTGTCGCTATCGCGAATTCGTGCTCAGAATACGACGATGTTAACCTTAGCTCCGCTTGCTGTTCGACCATTGATCGAA
CAGGAACTGGCTGTAGTTCAGCAGGCAATCGAACGTCAGCACTTAACGATCAATAACCAAGTCGATGCTGATCTAATGAT
AACGAGCGATTCACAGAAGTTGAGTCAGATCATCAAAAATCTGTTAACGAATGCAATCAAGTATAATCGACCTCATGGTA
GCGTGACGATTGCCACCATGGTCAGTCATCAAACGTGGTCGCTGATAGTTAAAGATACGGGCATTGGTATCAGTGCCGAT
GATCAACAGCGTATTTTTGAACGCTTCTATCGGGCGAGTCCGTCACGTTCACAGCAACTTGTGAGTGGGACTGGACTCGG
ATTAGCGATTGTTCAAGAATTGGTCAACGCAATGCACGGAACAATCGACGTTGTCAGTCAACGGGGCGTTGGGACAACTA
TGACAGTTACATTGCCACTAGTTGCGAGTGCTTCCGAGCAGTAG

Upstream 100 bases:

>100_bases
CACATCTCACATTTACGTGAAAAGATTGAAGCAGATCCCAAGCAGCCACGGTTGATCAGGACGGTCCGGGGTTTTGGCTA
CGAATTTGTGGGTGACGGCC

Downstream 100 bases:

>100_bases
TAAGGCGTTAAGCATACGGACAGTTCTGATTGTAACTGGTGGTCTATCAAATGATGACGTGGTGACGGTATGAGTTGTAT
TGCTGAGTCAAAAGATGTCA

Product: histidine protein kinase; sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MHNRWQQTTRLLLMENVLLVLIVLVVLQHFSPIKWSWRLVGCLVGAWLVLAVIEGLIAWRQQSERQRTIDRMEAKLRQMS
SQQFPPHILLPQSDPLYSLAQAVNQLESYQKSQTRRAELQEKELMMIMRYLPVGVMVIDHRRQVQLSNPAMSELLQVSVN
TVAHIYTKDIQLYALTKLIEDTITSGKNQRATITLIPAPNTQVVEASTVYIQGNRSQFQIVLMLYDITEVYMIEQMQDDF
VSNASHELKTPITAISGFTETLLSGAKDDPDTLDQFLHIIADESQRLIDLIQDVLSLSRIRAQNTTMLTLAPLAVRPLIE
QELAVVQQAIERQHLTINNQVDADLMITSDSQKLSQIIKNLLTNAIKYNRPHGSVTIATMVSHQTWSLIVKDTGIGISAD
DQQRIFERFYRASPSRSQQLVSGTGLGLAIVQELVNAMHGTIDVVSQRGVGTTMTVTLPLVASASEQ

Sequences:

>Translated_467_residues
MHNRWQQTTRLLLMENVLLVLIVLVVLQHFSPIKWSWRLVGCLVGAWLVLAVIEGLIAWRQQSERQRTIDRMEAKLRQMS
SQQFPPHILLPQSDPLYSLAQAVNQLESYQKSQTRRAELQEKELMMIMRYLPVGVMVIDHRRQVQLSNPAMSELLQVSVN
TVAHIYTKDIQLYALTKLIEDTITSGKNQRATITLIPAPNTQVVEASTVYIQGNRSQFQIVLMLYDITEVYMIEQMQDDF
VSNASHELKTPITAISGFTETLLSGAKDDPDTLDQFLHIIADESQRLIDLIQDVLSLSRIRAQNTTMLTLAPLAVRPLIE
QELAVVQQAIERQHLTINNQVDADLMITSDSQKLSQIIKNLLTNAIKYNRPHGSVTIATMVSHQTWSLIVKDTGIGISAD
DQQRIFERFYRASPSRSQQLVSGTGLGLAIVQELVNAMHGTIDVVSQRGVGTTMTVTLPLVASASEQ
>Mature_467_residues
MHNRWQQTTRLLLMENVLLVLIVLVVLQHFSPIKWSWRLVGCLVGAWLVLAVIEGLIAWRQQSERQRTIDRMEAKLRQMS
SQQFPPHILLPQSDPLYSLAQAVNQLESYQKSQTRRAELQEKELMMIMRYLPVGVMVIDHRRQVQLSNPAMSELLQVSVN
TVAHIYTKDIQLYALTKLIEDTITSGKNQRATITLIPAPNTQVVEASTVYIQGNRSQFQIVLMLYDITEVYMIEQMQDDF
VSNASHELKTPITAISGFTETLLSGAKDDPDTLDQFLHIIADESQRLIDLIQDVLSLSRIRAQNTTMLTLAPLAVRPLIE
QELAVVQQAIERQHLTINNQVDADLMITSDSQKLSQIIKNLLTNAIKYNRPHGSVTIATMVSHQTWSLIVKDTGIGISAD
DQQRIFERFYRASPSRSQQLVSGTGLGLAIVQELVNAMHGTIDVVSQRGVGTTMTVTLPLVASASEQ

Specific function: Member of the two-component regulatory system phoP/phoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates phoP in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=258, Percent_Identity=30.6201550387597, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI145693157, Length=237, Percent_Identity=30.8016877637131, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1788713, Length=230, Percent_Identity=31.7391304347826, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789149, Length=229, Percent_Identity=33.6244541484716, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788393, Length=228, Percent_Identity=31.140350877193, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081816, Length=246, Percent_Identity=28.4552845528455, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788549, Length=224, Percent_Identity=30.3571428571429, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=28.8288288288288, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI48994928, Length=478, Percent_Identity=23.8493723849372, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1786783, Length=241, Percent_Identity=29.8755186721992, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1790861, Length=215, Percent_Identity=30.2325581395349, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787894, Length=222, Percent_Identity=30.1801801801802, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082128, Length=232, Percent_Identity=27.5862068965517, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790346, Length=236, Percent_Identity=27.9661016949153, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1786912, Length=239, Percent_Identity=27.1966527196653, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1789403, Length=224, Percent_Identity=32.1428571428571, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788279, Length=223, Percent_Identity=23.7668161434978, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789808, Length=232, Percent_Identity=24.5689655172414, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=79, Percent_Identity=45.5696202531646, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52609; Mature: 52609

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNRWQQTTRLLLMENVLLVLIVLVVLQHFSPIKWSWRLVGCLVGAWLVLAVIEGLIAWR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QQSERQRTIDRMEAKLRQMSSQQFPPHILLPQSDPLYSLAQAVNQLESYQKSQTRRAELQ
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EKELMMIMRYLPVGVMVIDHRRQVQLSNPAMSELLQVSVNTVAHIYTKDIQLYALTKLIE
HHHHHHHHHHHCCCEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTITSGKNQRATITLIPAPNTQVVEASTVYIQGNRSQFQIVLMLYDITEVYMIEQMQDDF
HHHHCCCCCCEEEEEEECCCCEEEEEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHH
VSNASHELKTPITAISGFTETLLSGAKDDPDTLDQFLHIIADESQRLIDLIQDVLSLSRI
HCCCCHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
RAQNTTMLTLAPLAVRPLIEQELAVVQQAIERQHLTINNQVDADLMITSDSQKLSQIIKN
HHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEEEECCHHHHHHHHHH
LLTNAIKYNRPHGSVTIATMVSHQTWSLIVKDTGIGISADDQQRIFERFYRASPSRSQQL
HHHHHHHCCCCCCCEEEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHH
VSGTGLGLAIVQELVNAMHGTIDVVSQRGVGTTMTVTLPLVASASEQ
HHCCCCHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEEEEHHCCCCC
>Mature Secondary Structure
MHNRWQQTTRLLLMENVLLVLIVLVVLQHFSPIKWSWRLVGCLVGAWLVLAVIEGLIAWR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QQSERQRTIDRMEAKLRQMSSQQFPPHILLPQSDPLYSLAQAVNQLESYQKSQTRRAELQ
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EKELMMIMRYLPVGVMVIDHRRQVQLSNPAMSELLQVSVNTVAHIYTKDIQLYALTKLIE
HHHHHHHHHHHCCCEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTITSGKNQRATITLIPAPNTQVVEASTVYIQGNRSQFQIVLMLYDITEVYMIEQMQDDF
HHHHCCCCCCEEEEEEECCCCEEEEEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHH
VSNASHELKTPITAISGFTETLLSGAKDDPDTLDQFLHIIADESQRLIDLIQDVLSLSRI
HCCCCHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
RAQNTTMLTLAPLAVRPLIEQELAVVQQAIERQHLTINNQVDADLMITSDSQKLSQIIKN
HHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEEEECCHHHHHHHHHH
LLTNAIKYNRPHGSVTIATMVSHQTWSLIVKDTGIGISADDQQRIFERFYRASPSRSQQL
HHHHHHHCCCCCCCEEEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHH
VSGTGLGLAIVQELVNAMHGTIDVVSQRGVGTTMTVTLPLVASASEQ
HHCCCCHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3142862; 9387221; 9384377 [H]