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The map label for this gene is pstE [H]

Identifier: 28377605

GI number: 28377605

Start: 682984

End: 683859

Strand: Direct

Name: pstE [H]

Synonym: lp_0746

Alternate gene names: 28377605

Gene position: 682984-683859 (Clockwise)

Preceding gene: 28377604

Following gene: 28377606

Centisome position: 20.64

GC content: 45.32

Gene sequence:

>876_bases
ATGAGAAAAAGTCGGGTCATCCAATTAATCGTGTTAATCGCGATCATTGGACTAGTTGGATTAGGGTACACACATCGTGA
TCGAACTAGTGCCAGTGAATCAATTACCGCGGTTGGATCAACGGCCTTACAGCCACTGGTTGAAGCAGCCGGTGAACAAT
ACAGCAGCAACCATACCGGTGTGTTTATCAATGTGCAGGGTGGTGGCTCTGGAACGGGACTCAGTCAAATTCAGGCGGGG
GCCGTGGCAATCGGTAATTCTGATATTTTTGCCGAAGAACAGTCTGGCATTACAGCGAGCAAGTTAATTGATCACCGGGT
AGCCGTCGTCGGTATTGCCCCCATCGTTAATAAAAAGGCGGGCATTACCAATTTGACGCAGCAGCAACTCATTCAGATTT
TTACTGGCAAGGTCACGAATTGGCGTGAAGTTGGTGGACGTAATTTACCGATTACGTTAATTAACCGTGCTCAAGGCTCC
GGAACCCGGAAGACATTTGAGCGATACGCCTTAAAGGGTAATCAGAGTGTTGATTCCCAAGAACAAGATTCGTCAGGATT
AGCTCGGTCAATTGTTGCTAGTACACCTGGCGCAATTAGTTATGTGGCTTTTTCTTACTTAGACAACTCAGTCCAGACCG
TCAATGTTGATGGTATTCGGCCAACCGAAAAAAATGTGCGGAATAATCGCTGGTACATCTGGTCCTACGAACATTTATAT
ACGGGGAAGCACCCCAATCAGTTAACCAAAAAATTTATTGATTACATTTTATCAACCGCTGTTCAGCAGAAGTTAGTCAA
GCAGTTAGGTTATATTTCGGTCCATGACATGCAAGTACAGCGGAATGCGGCCGGTAAAGTTTCAGTTATGAAATGA

Upstream 100 bases:

>100_bases
AAATATATCGACGTTTACACAATCTTTACAAAACTCATATAAAGCATTTACACAAACTTTGTATTATTGAATATGAGCAA
TGGGTAGGAGGTATATAGTT

Downstream 100 bases:

>100_bases
GGGAGAGCGCATGGATAAAATTCGAGAAAGCTTATTAAAAAAATCAGTTGCTAGTAAAATTGAACGGCGTGGTAAGGCCA
TTAGTCTAGTTTGTCTAGCG

Product: phosphate ABC transporter, substrate binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP 1 [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MRKSRVIQLIVLIAIIGLVGLGYTHRDRTSASESITAVGSTALQPLVEAAGEQYSSNHTGVFINVQGGGSGTGLSQIQAG
AVAIGNSDIFAEEQSGITASKLIDHRVAVVGIAPIVNKKAGITNLTQQQLIQIFTGKVTNWREVGGRNLPITLINRAQGS
GTRKTFERYALKGNQSVDSQEQDSSGLARSIVASTPGAISYVAFSYLDNSVQTVNVDGIRPTEKNVRNNRWYIWSYEHLY
TGKHPNQLTKKFIDYILSTAVQQKLVKQLGYISVHDMQVQRNAAGKVSVMK

Sequences:

>Translated_291_residues
MRKSRVIQLIVLIAIIGLVGLGYTHRDRTSASESITAVGSTALQPLVEAAGEQYSSNHTGVFINVQGGGSGTGLSQIQAG
AVAIGNSDIFAEEQSGITASKLIDHRVAVVGIAPIVNKKAGITNLTQQQLIQIFTGKVTNWREVGGRNLPITLINRAQGS
GTRKTFERYALKGNQSVDSQEQDSSGLARSIVASTPGAISYVAFSYLDNSVQTVNVDGIRPTEKNVRNNRWYIWSYEHLY
TGKHPNQLTKKFIDYILSTAVQQKLVKQLGYISVHDMQVQRNAAGKVSVMK
>Mature_291_residues
MRKSRVIQLIVLIAIIGLVGLGYTHRDRTSASESITAVGSTALQPLVEAAGEQYSSNHTGVFINVQGGGSGTGLSQIQAG
AVAIGNSDIFAEEQSGITASKLIDHRVAVVGIAPIVNKKAGITNLTQQQLIQIFTGKVTNWREVGGRNLPITLINRAQGS
GTRKTFERYALKGNQSVDSQEQDSSGLARSIVASTPGAISYVAFSYLDNSVQTVNVDGIRPTEKNVRNNRWYIWSYEHLY
TGKHPNQLTKKFIDYILSTAVQQKLVKQLGYISVHDMQVQRNAAGKVSVMK

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=318, Percent_Identity=26.7295597484277, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011862 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 31528; Mature: 31528

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSRVIQLIVLIAIIGLVGLGYTHRDRTSASESITAVGSTALQPLVEAAGEQYSSNHTG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHCCCCCCE
VFINVQGGGSGTGLSQIQAGAVAIGNSDIFAEEQSGITASKLIDHRVAVVGIAPIVNKKA
EEEEEECCCCCCCHHHHCCCEEEECCCCEEECCCCCCHHHHHHHHHHHEEEEHHHHCCCC
GITNLTQQQLIQIFTGKVTNWREVGGRNLPITLINRAQGSGTRKTFERYALKGNQSVDSQ
CCCHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCC
EQDSSGLARSIVASTPGAISYVAFSYLDNSVQTVNVDGIRPTEKNVRNNRWYIWSYEHLY
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHCCCCCEEEEEEEHHEE
TGKHPNQLTKKFIDYILSTAVQQKLVKQLGYISVHDMQVQRNAAGKVSVMK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEECC
>Mature Secondary Structure
MRKSRVIQLIVLIAIIGLVGLGYTHRDRTSASESITAVGSTALQPLVEAAGEQYSSNHTG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHCCCCCCE
VFINVQGGGSGTGLSQIQAGAVAIGNSDIFAEEQSGITASKLIDHRVAVVGIAPIVNKKA
EEEEEECCCCCCCHHHHCCCEEEECCCCEEECCCCCCHHHHHHHHHHHEEEEHHHHCCCC
GITNLTQQQLIQIFTGKVTNWREVGGRNLPITLINRAQGSGTRKTFERYALKGNQSVDSQ
CCCHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCC
EQDSSGLARSIVASTPGAISYVAFSYLDNSVQTVNVDGIRPTEKNVRNNRWYIWSYEHLY
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHCCCCCEEEEEEEHHEE
TGKHPNQLTKKFIDYILSTAVQQKLVKQLGYISVHDMQVQRNAAGKVSVMK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]