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The map label for this gene is gltX

Identifier: 28377485

GI number: 28377485

Start: 579545

End: 581035

Strand: Direct

Name: gltX

Synonym: lp_0609

Alternate gene names: 28377485

Gene position: 579545-581035 (Clockwise)

Preceding gene: 28377484

Following gene: 28377486

Centisome position: 17.52

GC content: 44.94

Gene sequence:

>1491_bases
TTGGCAAAGAGCAAGATTCGTGTGCGTTACGCACCAAGTCCAACTGGCCATTTACACATTGGTAATGCTCGGACTGCATT
GTTTAACTATTTATTTGCCCGGCATAATAAGGGAAAGTTCATTCTTCGGATTGAAGACACCGATTTAAAGCGGAATGTTA
AAGACGGTGAAAAGAGTCAGATGGACAATTTGACGTGGTTAGGGATTGATTGGGACGAAGGTCCGGATAAGGGTGGCGAC
TTTGGCCCTTACCGTCAATCAGAACGGAAATCAATCTATGACCCGTTGATTCAACAATTAATTGATGAAGGTAAAGCTTA
CGAATCTTACATGACGGAAGACGAATTGTCGGCACAACGTGAAGCTCAAAAAGCGCGTAAGGAAATGCCACACTACGTCT
ACGAATTTGCTGGCATGACGGAAGACGAAAAACAGGCGAAGATCGCGGCTGCTAAGGCGGCTGGTATCCAACCCGTTATT
CGCTTCCACGTCCCAGAAAATACGACCTATGCTTGGGATGACATGGTTAAAGGTAAAGTTTCGTTTGAATCCAAGACGGT
TGGTGGCGACTTTGTCATCAAGAAGCGTGACGGGATGCCAACGTACAACTTTGCGGTGGTTGTTGATGACCATATGATGG
AAATTAGCCACGTCTTCCGTGGTGATGACCACGTTGCCAACACGCCTAAGCAATTGATGATTTATGAAGCCTTTGGTTGG
CAACCACCGAAGTTTGGCCACATGAGCTTAATTATCAATACCGAAACCGGTAAGAAGTTAAGCAAGCGTGACGAAACCGT
ATTGCAATTTATCGAACAATACCGTGAATTGGGTTACTTACCAGAAGCAATGCTTAACTTCATCATCCTACTAGGATGGT
CACCAGTTGGCGAAAGTGAATTGTTCAGTAAGCGCGAATTCATCAAAATGTATGATGAAAAGCGGTTGAGCAAGTCACCA
GCAGCTTTTGATGGCAAGAAGTTGGAATGGGTGAACAACCAGTACATTAAGAAGGCTGACGAAAATGAAGTCTTTGCAAT
GTCAATTCGCCAATTGATCAAGGCTGGCCGGTTACCACAACGCCCAGATATGAGCCAGATTGAATGGACACGGACGTTGG
TTTCATTATTTAAGAACCAGATGAGCTATACTGGTCAAATCGTTGATTTAGCAGATCTCTTCTTCAATGGTCCGGCTGAT
ATCAATGATGAAGCTAAGGCCGAACTTGATAATGACACGGCACTCCCTGTCTTGAAGGAATTCCGGCAACGAATCGAAGA
TATTGACGTCTTCGAAGCAACCGCAATTCAAAAGACGATCAAGAGCATTCAAAAGGATACGAAGATCAAGGGCCGGGCAT
TATACATGCCGATCCGGATCGCCGTTTCTCACGAAATGCACGGGCCAGAATTACCAGAAACGATTGAATTATTAGGGCGT
GAAACGACCAAGAAGCATCTCGATGCTATGATTGCGGAACTCGCCAAGTAA

Upstream 100 bases:

>100_bases
GTAAACTGGAAAAGCAAGTGAAAGTCGGAATATTCTGGTCGATAGAAAGGTTCCGGCGTAACATTTAAGATGAAAAAATA
CGAAAGAGGCGTTAATCACA

Downstream 100 bases:

>100_bases
TGAATGGATTATTAAAATATTAGTTGGTTGTCGTAAGTGGCGGTCGTCGTCAGCAAGACAACGCAATTAATACATCGTGC
GGTCAGCAAGCTAGTCCCCG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MAKSKIRVRYAPSPTGHLHIGNARTALFNYLFARHNKGKFILRIEDTDLKRNVKDGEKSQMDNLTWLGIDWDEGPDKGGD
FGPYRQSERKSIYDPLIQQLIDEGKAYESYMTEDELSAQREAQKARKEMPHYVYEFAGMTEDEKQAKIAAAKAAGIQPVI
RFHVPENTTYAWDDMVKGKVSFESKTVGGDFVIKKRDGMPTYNFAVVVDDHMMEISHVFRGDDHVANTPKQLMIYEAFGW
QPPKFGHMSLIINTETGKKLSKRDETVLQFIEQYRELGYLPEAMLNFIILLGWSPVGESELFSKREFIKMYDEKRLSKSP
AAFDGKKLEWVNNQYIKKADENEVFAMSIRQLIKAGRLPQRPDMSQIEWTRTLVSLFKNQMSYTGQIVDLADLFFNGPAD
INDEAKAELDNDTALPVLKEFRQRIEDIDVFEATAIQKTIKSIQKDTKIKGRALYMPIRIAVSHEMHGPELPETIELLGR
ETTKKHLDAMIAELAK

Sequences:

>Translated_496_residues
MAKSKIRVRYAPSPTGHLHIGNARTALFNYLFARHNKGKFILRIEDTDLKRNVKDGEKSQMDNLTWLGIDWDEGPDKGGD
FGPYRQSERKSIYDPLIQQLIDEGKAYESYMTEDELSAQREAQKARKEMPHYVYEFAGMTEDEKQAKIAAAKAAGIQPVI
RFHVPENTTYAWDDMVKGKVSFESKTVGGDFVIKKRDGMPTYNFAVVVDDHMMEISHVFRGDDHVANTPKQLMIYEAFGW
QPPKFGHMSLIINTETGKKLSKRDETVLQFIEQYRELGYLPEAMLNFIILLGWSPVGESELFSKREFIKMYDEKRLSKSP
AAFDGKKLEWVNNQYIKKADENEVFAMSIRQLIKAGRLPQRPDMSQIEWTRTLVSLFKNQMSYTGQIVDLADLFFNGPAD
INDEAKAELDNDTALPVLKEFRQRIEDIDVFEATAIQKTIKSIQKDTKIKGRALYMPIRIAVSHEMHGPELPETIELLGR
ETTKKHLDAMIAELAK
>Mature_495_residues
AKSKIRVRYAPSPTGHLHIGNARTALFNYLFARHNKGKFILRIEDTDLKRNVKDGEKSQMDNLTWLGIDWDEGPDKGGDF
GPYRQSERKSIYDPLIQQLIDEGKAYESYMTEDELSAQREAQKARKEMPHYVYEFAGMTEDEKQAKIAAAKAAGIQPVIR
FHVPENTTYAWDDMVKGKVSFESKTVGGDFVIKKRDGMPTYNFAVVVDDHMMEISHVFRGDDHVANTPKQLMIYEAFGWQ
PPKFGHMSLIINTETGKKLSKRDETVLQFIEQYRELGYLPEAMLNFIILLGWSPVGESELFSKREFIKMYDEKRLSKSPA
AFDGKKLEWVNNQYIKKADENEVFAMSIRQLIKAGRLPQRPDMSQIEWTRTLVSLFKNQMSYTGQIVDLADLFFNGPADI
NDEAKAELDNDTALPVLKEFRQRIEDIDVFEATAIQKTIKSIQKDTKIKGRALYMPIRIAVSHEMHGPELPETIELLGRE
TTKKHLDAMIAELAK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=343, Percent_Identity=38.7755102040816, Blast_Score=242, Evalue=7e-64,
Organism=Homo sapiens, GI62241042, Length=263, Percent_Identity=25.8555133079848, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788743, Length=496, Percent_Identity=36.0887096774194, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI226510925, Length=266, Percent_Identity=29.6992481203008, Blast_Score=126, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71993394, Length=502, Percent_Identity=34.2629482071713, Blast_Score=246, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6324540, Length=506, Percent_Identity=32.6086956521739, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6321192, Length=216, Percent_Identity=25, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24665321, Length=502, Percent_Identity=33.6653386454183, Blast_Score=275, Evalue=4e-74,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_LACPL (Q88YY0)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784377.1
- ProteinModelPortal:   Q88YY0
- SMR:   Q88YY0
- GeneID:   1061586
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0609
- NMPDR:   fig|220668.1.peg.511
- HOGENOM:   HBG628189
- OMA:   WINNQYV
- ProtClustDB:   PRK01406
- BioCyc:   LPLA220668:LP_0609-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 56920; Mature: 56789

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 262-262

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSKIRVRYAPSPTGHLHIGNARTALFNYLFARHNKGKFILRIEDTDLKRNVKDGEKSQ
CCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCHHCCCCCCCHHH
MDNLTWLGIDWDEGPDKGGDFGPYRQSERKSIYDPLIQQLIDEGKAYESYMTEDELSAQR
HCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHH
EAQKARKEMPHYVYEFAGMTEDEKQAKIAAAKAAGIQPVIRFHVPENTTYAWDDMVKGKV
HHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHCCEE
SFESKTVGGDFVIKKRDGMPTYNFAVVVDDHMMEISHVFRGDDHVANTPKQLMIYEAFGW
EECCEECCCCEEEEECCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCHHEEEEEECCC
QPPKFGHMSLIINTETGKKLSKRDETVLQFIEQYRELGYLPEAMLNFIILLGWSPVGESE
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEHCCCCCCCHHH
LFSKREFIKMYDEKRLSKSPAAFDGKKLEWVNNQYIKKADENEVFAMSIRQLIKAGRLPQ
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHCCHHHHCCCCCCHHHHHHHHHHHHCCCCC
RPDMSQIEWTRTLVSLFKNQMSYTGQIVDLADLFFNGPADINDEAKAELDNDTALPVLKE
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHH
FRQRIEDIDVFEATAIQKTIKSIQKDTKIKGRALYMPIRIAVSHEMHGPELPETIELLGR
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHCC
ETTKKHLDAMIAELAK
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKSKIRVRYAPSPTGHLHIGNARTALFNYLFARHNKGKFILRIEDTDLKRNVKDGEKSQ
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCHHCCCCCCCHHH
MDNLTWLGIDWDEGPDKGGDFGPYRQSERKSIYDPLIQQLIDEGKAYESYMTEDELSAQR
HCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHH
EAQKARKEMPHYVYEFAGMTEDEKQAKIAAAKAAGIQPVIRFHVPENTTYAWDDMVKGKV
HHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHCCEE
SFESKTVGGDFVIKKRDGMPTYNFAVVVDDHMMEISHVFRGDDHVANTPKQLMIYEAFGW
EECCEECCCCEEEEECCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCHHEEEEEECCC
QPPKFGHMSLIINTETGKKLSKRDETVLQFIEQYRELGYLPEAMLNFIILLGWSPVGESE
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEHCCCCCCCHHH
LFSKREFIKMYDEKRLSKSPAAFDGKKLEWVNNQYIKKADENEVFAMSIRQLIKAGRLPQ
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHCCHHHHCCCCCCHHHHHHHHHHHHCCCCC
RPDMSQIEWTRTLVSLFKNQMSYTGQIVDLADLFFNGPADINDEAKAELDNDTALPVLKE
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHH
FRQRIEDIDVFEATAIQKTIKSIQKDTKIKGRALYMPIRIAVSHEMHGPELPETIELLGR
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHCC
ETTKKHLDAMIAELAK
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566