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The map label for this gene is cysS

Identifier: 28377486

GI number: 28377486

Start: 581313

End: 582725

Strand: Direct

Name: cysS

Synonym: lp_0610

Alternate gene names: 28377486

Gene position: 581313-582725 (Clockwise)

Preceding gene: 28377485

Following gene: 28377487

Centisome position: 17.57

GC content: 45.93

Gene sequence:

>1413_bases
ATGTTAAGTGTTTTTAATACGTTAACGCGTCAAAAAGAACCGTTTAAGCCTATCACCCCCGGAGTCGTGAAAATGTATGT
CTGTGGGCCGACCGTTTATAACTATATTCATATCGGTAATGCGCGTTCTGCAATTGCCTTTGATACTATCCGACGGTATT
TTGAATATTGCGGTTACCACGTTGACTATGTCTCCAACTTCACGGATGTGGATGATAAGCTGATCAAACGTGCCGCTGAA
GATCACACCACGGTACCAGCAGTTGCTGACCGGTTTATTAAAGCTTTTATGGAAGATACGACTGCGGTCAATATTGAACC
AGCGACGGTTCACCCGCGGGCCTCTGAGAATATCCCAGAAATCATTGCATTTGTACAAGCATTGATTGAAAAGGGCTATG
CTTACGAATCAGCTGGCGACGTATATTACCGCGCACGGAAGTTTAAAAAGTATGGTCAGTTATCTGACCAGCGAATCGAT
GATTTAGAAGTGGGTGCCAGTCAGCATACTGCCGATGAAGAAACCGATCGTAAAGAAGATCCAATTGACTTTGCACTCTG
GAAAGCAGCCAAGCCCGGTGAAATTTCATGGGAATCGCCATGGGGTGCGGGTCGGCCCGGCTGGCATATTGAATGCTCGG
TCATGTCTACTAAGTACTTGGGCGATACGTTTGATATTCATGGTGGTGGTCAGGACCTTGAATTCCCACATCATGAAAAT
GAAATTGCCCAGAGTGAAGCGAAGACCGGCAAGACGTTTGCCAATTATTGGTTACACAACGGTTTTGTGACGGTTGGCGA
TGACAACGAAAAGATGAGTAAGTCATTAGGTAATTTTGTGACGGTTCACGATATTATTAAAACGGTTGATCCGCAAGTGC
TACGGTTCTTCATGGCGACGACCCAGTACCGGCGACCGATTCAATATACGCAGGCCAACTTAGACGAAGCAGCTAATAAC
TTGGATAAGTTACGTAATGCTTATCGTAATCTCACGTTCCGGTTGCAAGATGCGACGAACGGGACGGATACTGCGGTGGC
AGAACAGTTACAGACACTCATCACTAACTTTGGAACCGCAATGGATGACGATTTCAATGTTCAAAACGGGGTGGCTGCTG
TGTATGAATTAGCTAAGTTAGCCAATGTCTATGCAGCCAAGGCAACGGTTCAAAAAGAGACGTTGATTGCCTTACAGAAG
GCATTAGTGCAGTTGACTGGCGTCTTCGGCGTCACTTTTGAAACGGCTAATGCGACGCTGGCTGATGACGCGATTCAAGC
ACTGATCGATGAACGAGAAGCTGCTCGTAAAGCTAAGGATTTTGCTAAGGCCGACCAGATTCGTGATGATTTGAAAGTTC
AGGGGATTATGTTAGAAGATACGCCTCAGGGTGTGCGGTTTAGAAAGGAATAA

Upstream 100 bases:

>100_bases
GTTTCGGTTCCGCCGTGCGAGGGATGCTTTTTATTTGTGTTTCAAACATGGTAAACTATCATCAAAAGTAATGATTCAAT
TGAAAGGAGTTGTCGCCAAT

Downstream 100 bases:

>100_bases
TATGCAAGTTGATTATCAACAATTAAACGGGATTGCACTCGCGTACCTCGGTGACGCGGTGTATGAACCGTTTATTCGCC
AACATTTGATTGAAAGTGGT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MLSVFNTLTRQKEPFKPITPGVVKMYVCGPTVYNYIHIGNARSAIAFDTIRRYFEYCGYHVDYVSNFTDVDDKLIKRAAE
DHTTVPAVADRFIKAFMEDTTAVNIEPATVHPRASENIPEIIAFVQALIEKGYAYESAGDVYYRARKFKKYGQLSDQRID
DLEVGASQHTADEETDRKEDPIDFALWKAAKPGEISWESPWGAGRPGWHIECSVMSTKYLGDTFDIHGGGQDLEFPHHEN
EIAQSEAKTGKTFANYWLHNGFVTVGDDNEKMSKSLGNFVTVHDIIKTVDPQVLRFFMATTQYRRPIQYTQANLDEAANN
LDKLRNAYRNLTFRLQDATNGTDTAVAEQLQTLITNFGTAMDDDFNVQNGVAAVYELAKLANVYAAKATVQKETLIALQK
ALVQLTGVFGVTFETANATLADDAIQALIDEREAARKAKDFAKADQIRDDLKVQGIMLEDTPQGVRFRKE

Sequences:

>Translated_470_residues
MLSVFNTLTRQKEPFKPITPGVVKMYVCGPTVYNYIHIGNARSAIAFDTIRRYFEYCGYHVDYVSNFTDVDDKLIKRAAE
DHTTVPAVADRFIKAFMEDTTAVNIEPATVHPRASENIPEIIAFVQALIEKGYAYESAGDVYYRARKFKKYGQLSDQRID
DLEVGASQHTADEETDRKEDPIDFALWKAAKPGEISWESPWGAGRPGWHIECSVMSTKYLGDTFDIHGGGQDLEFPHHEN
EIAQSEAKTGKTFANYWLHNGFVTVGDDNEKMSKSLGNFVTVHDIIKTVDPQVLRFFMATTQYRRPIQYTQANLDEAANN
LDKLRNAYRNLTFRLQDATNGTDTAVAEQLQTLITNFGTAMDDDFNVQNGVAAVYELAKLANVYAAKATVQKETLIALQK
ALVQLTGVFGVTFETANATLADDAIQALIDEREAARKAKDFAKADQIRDDLKVQGIMLEDTPQGVRFRKE
>Mature_470_residues
MLSVFNTLTRQKEPFKPITPGVVKMYVCGPTVYNYIHIGNARSAIAFDTIRRYFEYCGYHVDYVSNFTDVDDKLIKRAAE
DHTTVPAVADRFIKAFMEDTTAVNIEPATVHPRASENIPEIIAFVQALIEKGYAYESAGDVYYRARKFKKYGQLSDQRID
DLEVGASQHTADEETDRKEDPIDFALWKAAKPGEISWESPWGAGRPGWHIECSVMSTKYLGDTFDIHGGGQDLEFPHHEN
EIAQSEAKTGKTFANYWLHNGFVTVGDDNEKMSKSLGNFVTVHDIIKTVDPQVLRFFMATTQYRRPIQYTQANLDEAANN
LDKLRNAYRNLTFRLQDATNGTDTAVAEQLQTLITNFGTAMDDDFNVQNGVAAVYELAKLANVYAAKATVQKETLIALQK
ALVQLTGVFGVTFETANATLADDAIQALIDEREAARKAKDFAKADQIRDDLKVQGIMLEDTPQGVRFRKE

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=430, Percent_Identity=37.6744186046512, Blast_Score=272, Evalue=6e-73,
Organism=Homo sapiens, GI62240992, Length=413, Percent_Identity=33.4140435835351, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI21269877, Length=413, Percent_Identity=33.4140435835351, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI303304987, Length=413, Percent_Identity=33.6561743341404, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI10835051, Length=413, Percent_Identity=33.4140435835351, Blast_Score=200, Evalue=2e-51,
Organism=Escherichia coli, GI1786737, Length=481, Percent_Identity=45.945945945946, Blast_Score=388, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17509797, Length=331, Percent_Identity=34.1389728096677, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17509795, Length=331, Percent_Identity=34.1389728096677, Blast_Score=192, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6324082, Length=343, Percent_Identity=36.1516034985423, Blast_Score=207, Evalue=3e-54,
Organism=Drosophila melanogaster, GI19922218, Length=500, Percent_Identity=31.8, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI19922364, Length=368, Percent_Identity=33.4239130434783, Blast_Score=194, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_LACPL (Q88YX9)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784378.1
- ProteinModelPortal:   Q88YX9
- SMR:   Q88YX9
- GeneID:   1061584
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0610
- NMPDR:   fig|220668.1.peg.512
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   LPLA220668:LP_0610-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52765; Mature: 52765

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVFNTLTRQKEPFKPITPGVVKMYVCGPTVYNYIHIGNARSAIAFDTIRRYFEYCGYH
CCCHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHEEEECCCCHHHHHHHHHHHHHHHCCH
VDYVSNFTDVDDKLIKRAAEDHTTVPAVADRFIKAFMEDTTAVNIEPATVHPRASENIPE
HHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCEECCCCCCCHHH
IIAFVQALIEKGYAYESAGDVYYRARKFKKYGQLSDQRIDDLEVGASQHTADEETDRKED
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCC
PIDFALWKAAKPGEISWESPWGAGRPGWHIECSVMSTKYLGDTFDIHGGGQDLEFPHHEN
CCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHCCCCEEECCCCCCCCCCCCHH
EIAQSEAKTGKTFANYWLHNGFVTVGDDNEKMSKSLGNFVTVHDIIKTVDPQVLRFFMAT
HHHHHHHHCCHHHHHHHHHCCEEEECCCHHHHHHHHCCEEHHHHHHHHCCHHHHHHHHHH
TQYRRPIQYTQANLDEAANNLDKLRNAYRNLTFRLQDATNGTDTAVAEQLQTLITNFGTA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCC
MDDDFNVQNGVAAVYELAKLANVYAAKATVQKETLIALQKALVQLTGVFGVTFETANATL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHH
ADDAIQALIDEREAARKAKDFAKADQIRDDLKVQGIMLEDTPQGVRFRKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLSVFNTLTRQKEPFKPITPGVVKMYVCGPTVYNYIHIGNARSAIAFDTIRRYFEYCGYH
CCCHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHEEEECCCCHHHHHHHHHHHHHHHCCH
VDYVSNFTDVDDKLIKRAAEDHTTVPAVADRFIKAFMEDTTAVNIEPATVHPRASENIPE
HHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCEECCCCCCCHHH
IIAFVQALIEKGYAYESAGDVYYRARKFKKYGQLSDQRIDDLEVGASQHTADEETDRKED
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCC
PIDFALWKAAKPGEISWESPWGAGRPGWHIECSVMSTKYLGDTFDIHGGGQDLEFPHHEN
CCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHCCCCEEECCCCCCCCCCCCHH
EIAQSEAKTGKTFANYWLHNGFVTVGDDNEKMSKSLGNFVTVHDIIKTVDPQVLRFFMAT
HHHHHHHHCCHHHHHHHHHCCEEEECCCHHHHHHHHCCEEHHHHHHHHCCHHHHHHHHHH
TQYRRPIQYTQANLDEAANNLDKLRNAYRNLTFRLQDATNGTDTAVAEQLQTLITNFGTA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCC
MDDDFNVQNGVAAVYELAKLANVYAAKATVQKETLIALQKALVQLTGVFGVTFETANATL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHH
ADDAIQALIDEREAARKAKDFAKADQIRDDLKVQGIMLEDTPQGVRFRKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566