Click here to switch to the map view.

The map label for this gene is hisH [H]

Identifier: 28199150

GI number: 28199150

Start: 1473222

End: 1473824

Strand: Reverse

Name: hisH [H]

Synonym: PD1264

Alternate gene names: 28199150

Gene position: 1473824-1473222 (Counterclockwise)

Preceding gene: 28199151

Following gene: 28199149

Centisome position: 58.49

GC content: 61.36

Gene sequence:

>603_bases
ATGACCGAGGTTGCTTTGATTGATGCCGGTGGTGCCAATCTTGGTTCGGTGCGCTACGCCTTGCAGCGTTTGGGGGTAGA
ACCGCGCTTAGTGTGCGATGCCCGCGGACTGGAGGGGGCTGCGCGGGTGATTCTCCCCGGGGTCGGGAGCGCACCTGAAG
CGATGGCACGGCTCAACAATCAAGGCTTGATTGAGCCATTGCTCCGGCTACAGGTGCCGCTGATTGGGATCTGCCTGGGC
ATGCAATTGTTGTTTGAGCACTCTGAGGAAGGCGATGTGCCCTGCTTGGGATTGCTGCCTGGCCGCGTCCGCCGCCTGAC
GCCGGCTCCTAGCATCCGTGTGCCGCACATGGGCTGGAATCGCTTGTTGCCGCTGCGCGCTTCGCCATTGCTGGCGGAGG
TGCCCGAGGGGGCGAACGCTTATTTTGTGCATAGCTATGCAGTGCCCTTGACCACGGCAGCGGTGGCCGCCTGCGATCAC
GGCGGCATGTTTACCGCGATTGTGCAGCAGGGAGTGCGTTGTGGCGCGCAATTCCATCCAGAGCGTTCGGCCGAAACAGG
CGCACGTATCCTGCGTAATTTTCTTGAAATGGATGCCGCATGA

Upstream 100 bases:

>100_bases
ACCATCACAAAGTAGAAGCGTGTTTCAAGGCGCTGGCGCGTGCATTGCGTCAGGCGTTACAACGCCACGGCCACGTGCTT
CCTTCTACCAAGGGGGCGTT

Downstream 100 bases:

>100_bases
ATTTCATTGTCTATCCAGCCTTGGACATCCGTAACGGTGCTGTGGTGCGTCTCCAGCAGGGGGATTACGCGCGCCAGACG
CGCTATGACGATCAGGTATT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MTEVALIDAGGANLGSVRYALQRLGVEPRLVCDARGLEGAARVILPGVGSAPEAMARLNNQGLIEPLLRLQVPLIGICLG
MQLLFEHSEEGDVPCLGLLPGRVRRLTPAPSIRVPHMGWNRLLPLRASPLLAEVPEGANAYFVHSYAVPLTTAAVAACDH
GGMFTAIVQQGVRCGAQFHPERSAETGARILRNFLEMDAA

Sequences:

>Translated_200_residues
MTEVALIDAGGANLGSVRYALQRLGVEPRLVCDARGLEGAARVILPGVGSAPEAMARLNNQGLIEPLLRLQVPLIGICLG
MQLLFEHSEEGDVPCLGLLPGRVRRLTPAPSIRVPHMGWNRLLPLRASPLLAEVPEGANAYFVHSYAVPLTTAAVAACDH
GGMFTAIVQQGVRCGAQFHPERSAETGARILRNFLEMDAA
>Mature_199_residues
TEVALIDAGGANLGSVRYALQRLGVEPRLVCDARGLEGAARVILPGVGSAPEAMARLNNQGLIEPLLRLQVPLIGICLGM
QLLFEHSEEGDVPCLGLLPGRVRRLTPAPSIRVPHMGWNRLLPLRASPLLAEVPEGANAYFVHSYAVPLTTAAVAACDHG
GMFTAIVQQGVRCGAQFHPERSAETGARILRNFLEMDAA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=196, Percent_Identity=43.3673469387755, Blast_Score=159, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319725, Length=216, Percent_Identity=30.0925925925926, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21288; Mature: 21157

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVALIDAGGANLGSVRYALQRLGVEPRLVCDARGLEGAARVILPGVGSAPEAMARLNN
CCEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCCCCCHHHHHHHCC
QGLIEPLLRLQVPLIGICLGMQLLFEHSEEGDVPCLGLLPGRVRRLTPAPSIRVPHMGWN
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHCCCCCCEECCCCCCC
RLLPLRASPLLAEVPEGANAYFVHSYAVPLTTAAVAACDHGGMFTAIVQQGVRCGAQFHP
CCCCCCCCCHHHCCCCCCCEEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
ERSAETGARILRNFLEMDAA
CCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEVALIDAGGANLGSVRYALQRLGVEPRLVCDARGLEGAARVILPGVGSAPEAMARLNN
CEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCCCCCHHHHHHHCC
QGLIEPLLRLQVPLIGICLGMQLLFEHSEEGDVPCLGLLPGRVRRLTPAPSIRVPHMGWN
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHCCCCCCEECCCCCCC
RLLPLRASPLLAEVPEGANAYFVHSYAVPLTTAAVAACDHGGMFTAIVQQGVRCGAQFHP
CCCCCCCCCHHHCCCCCCCEEEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
ERSAETGARILRNFLEMDAA
CCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]