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The map label for this gene is btuE [H]

Identifier: 28199059

GI number: 28199059

Start: 1372474

End: 1373052

Strand: Reverse

Name: btuE [H]

Synonym: PD1169

Alternate gene names: 28199059

Gene position: 1373052-1372474 (Counterclockwise)

Preceding gene: 28199063

Following gene: 28199058

Centisome position: 54.49

GC content: 49.91

Gene sequence:

>579_bases
ATGAACATGCTCAATGTGTTGAGGTTTCCCGTTTTGGCTTTGGCTTTGGCTTTTGTTTCTAGTGGTGTACTGGCCGATGT
GGGGAGTTTGCTGGATTTGGATTACCGTCCGTTGGCCGGTAAAGAGACTGTCAATTTGCAGCGCCTTTATGGTGGCAAGG
TGCTGTTGGTGGTCAATACCGCCAGTAAATGTGGTTTTACCCCGCAATACGAAGGATTAGAGGCATTGCACCAGAAATTG
TCGCCACTTGGTTTTGCTGTACTTGGTTTTCCATCCAACGACTTTAAGGGGCAAGAACCAGGTGATGAGCAGCAAATTCA
GGAATTCTGCACGCTGACGTATGGGGTTAAGTTTCCAATGTTTCAGAAGGTTCACGTCAAAGGCAATGAGGTCACGCCAC
TGTATCAGCGCTTGACGCAGACGACGGGTGTGGCACCGGGCTGGAACTTTCACAAGTACCTCATTGCGCGTGATGGCCAT
GTTGTGGCTCAGTTTGATAGCCGTACGCGGCCTGATGATCCAATTCTGTTGGAAGCGATCAAGCGTGAGTTGGCAAAGTC
GGTGCAGGTAGTGCACTGA

Upstream 100 bases:

>100_bases
AGGTAAAAAATTTCGCACGATATGCAGGCAATGACCTGTGATGCGCAAGCGATGGCGATACAGTGACCTGATTGATGGTG
CCTGCTCCTAGGGAGAATAG

Downstream 100 bases:

>100_bases
TTGGGCACACGTGCAGCAACGGCTTGTATCCCTGCGGCAGTCGTTGTTGCTCCCATTGGGAAGACACGTTGCTGAGTGAA
GGTGTGTGCTGGAATACGCT

Product: glutathione peroxidase/vitamin B12 uptake permease

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MNMLNVLRFPVLALALAFVSSGVLADVGSLLDLDYRPLAGKETVNLQRLYGGKVLLVVNTASKCGFTPQYEGLEALHQKL
SPLGFAVLGFPSNDFKGQEPGDEQQIQEFCTLTYGVKFPMFQKVHVKGNEVTPLYQRLTQTTGVAPGWNFHKYLIARDGH
VVAQFDSRTRPDDPILLEAIKRELAKSVQVVH

Sequences:

>Translated_192_residues
MNMLNVLRFPVLALALAFVSSGVLADVGSLLDLDYRPLAGKETVNLQRLYGGKVLLVVNTASKCGFTPQYEGLEALHQKL
SPLGFAVLGFPSNDFKGQEPGDEQQIQEFCTLTYGVKFPMFQKVHVKGNEVTPLYQRLTQTTGVAPGWNFHKYLIARDGH
VVAQFDSRTRPDDPILLEAIKRELAKSVQVVH
>Mature_192_residues
MNMLNVLRFPVLALALAFVSSGVLADVGSLLDLDYRPLAGKETVNLQRLYGGKVLLVVNTASKCGFTPQYEGLEALHQKL
SPLGFAVLGFPSNDFKGQEPGDEQQIQEFCTLTYGVKFPMFQKVHVKGNEVTPLYQRLTQTTGVAPGWNFHKYLIARDGH
VVAQFDSRTRPDDPILLEAIKRELAKSVQVVH

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI15618997, Length=124, Percent_Identity=49.1935483870968, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI192455698, Length=166, Percent_Identity=37.3493975903614, Blast_Score=109, Evalue=1e-24,
Organism=Homo sapiens, GI75709200, Length=135, Percent_Identity=38.5185185185185, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI90903240, Length=135, Percent_Identity=38.5185185185185, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI90903238, Length=117, Percent_Identity=39.3162393162393, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI4557629, Length=166, Percent_Identity=31.3253012048193, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI6006001, Length=197, Percent_Identity=30.4568527918782, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1788003, Length=169, Percent_Identity=44.3786982248521, Blast_Score=146, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17535473, Length=146, Percent_Identity=38.3561643835616, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17506887, Length=152, Percent_Identity=37.5, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71993584, Length=185, Percent_Identity=31.8918918918919, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI193210707, Length=154, Percent_Identity=35.7142857142857, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17550320, Length=184, Percent_Identity=26.6304347826087, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319721, Length=148, Percent_Identity=45.945945945946, Blast_Score=133, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322228, Length=143, Percent_Identity=45.4545454545455, Blast_Score=128, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6322826, Length=138, Percent_Identity=37.6811594202899, Blast_Score=106, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24656772, Length=180, Percent_Identity=32.7777777777778, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24656767, Length=169, Percent_Identity=32.5443786982249, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24656777, Length=161, Percent_Identity=34.1614906832298, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI85726443, Length=124, Percent_Identity=37.0967741935484, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: NA

Molecular weight: Translated: 21272; Mature: 21272

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00460 GLUTATHIONE_PEROXID_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMLNVLRFPVLALALAFVSSGVLADVGSLLDLDYRPLAGKETVNLQRLYGGKVLLVVNT
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHEECCCEEEEEEEC
ASKCGFTPQYEGLEALHQKLSPLGFAVLGFPSNDFKGQEPGDEQQIQEFCTLTYGVKFPM
CHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
FQKVHVKGNEVTPLYQRLTQTTGVAPGWNFHKYLIARDGHVVAQFDSRTRPDDPILLEAI
EEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCHHHHHHH
KRELAKSVQVVH
HHHHHHHHCCCC
>Mature Secondary Structure
MNMLNVLRFPVLALALAFVSSGVLADVGSLLDLDYRPLAGKETVNLQRLYGGKVLLVVNT
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHEECCCEEEEEEEC
ASKCGFTPQYEGLEALHQKLSPLGFAVLGFPSNDFKGQEPGDEQQIQEFCTLTYGVKFPM
CHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
FQKVHVKGNEVTPLYQRLTQTTGVAPGWNFHKYLIARDGHVVAQFDSRTRPDDPILLEAI
EEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCHHHHHHH
KRELAKSVQVVH
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]