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The map label for this gene is mrpE

Identifier: 255767721

GI number: 255767721

Start: 3251248

End: 3251724

Strand: Direct

Name: mrpE

Synonym: BSU31640

Alternate gene names: 255767721

Gene position: 3251248-3251724 (Clockwise)

Preceding gene: 255767720

Following gene: 255767722

Centisome position: 77.12

GC content: 39.41

Gene sequence:

>477_bases
ATGGCATTTCAAATTTTATTAAATGTGTTTCTCGCTTTTTGCTGGATGTTCTTAAGCAACAGTCCAAGCGCCGCAGGATT
CATTACAGGCTATATTCTGGGAATGCTCTCTCTCTTTTTCTTCCGGCGCTTTTTCACTCGCCAGTTTTATCTATGGAAAT
TGATTTCTATTATCAAGCTCTGTTTCATTTTTATAAAGGAACTGTACCTTGCCAATGTCAGTGTGATGAAATCAGTCTTA
TCTCCAAAACTGAATATCAGACCGGGTATTTTTGCTTTTAAAACTGAACTGACGAAGGACTGGGAAATCACCATGCTTTC
TCTGCTCATTACATTAACGCCGGGAACCTTGGTGATGGATATTTCAGATGACAGAACAATACTTTATATTCATGCAATGG
ATATCGAGGATGCGGAAAAAGCGATTTTTGACATTCGGGAGTCATTTGAAAAAGCCATACAGGAGGTGAGCCGGTGA

Upstream 100 bases:

>100_bases
GATTGGGTACAGAATGGGTGTCGCCTTACGTTGATCAAGCGGCCGAGACGCTGCTGAATCCGGAAAAATATATCGAAGCT
GTTCTGAAGGAGTAGATCGC

Downstream 100 bases:

>100_bases
TGTTTACGCTGATCCTGCAAATCGCGCTCGGCATTATGGCAGTGTCTACCTTTTTGTATGTCATTCGTGTCATTAAAGGT
CCGACTGTGCCTGACCGGGT

Product: monovalent cation/H+ antiporter subunit E

Products: NA

Alternate protein names: Mrp complex subunit E; Multiple resistance and pH homeostasis protein E

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MAFQILLNVFLAFCWMFLSNSPSAAGFITGYILGMLSLFFFRRFFTRQFYLWKLISIIKLCFIFIKELYLANVSVMKSVL
SPKLNIRPGIFAFKTELTKDWEITMLSLLITLTPGTLVMDISDDRTILYIHAMDIEDAEKAIFDIRESFEKAIQEVSR

Sequences:

>Translated_158_residues
MAFQILLNVFLAFCWMFLSNSPSAAGFITGYILGMLSLFFFRRFFTRQFYLWKLISIIKLCFIFIKELYLANVSVMKSVL
SPKLNIRPGIFAFKTELTKDWEITMLSLLITLTPGTLVMDISDDRTILYIHAMDIEDAEKAIFDIRESFEKAIQEVSR
>Mature_157_residues
AFQILLNVFLAFCWMFLSNSPSAAGFITGYILGMLSLFFFRRFFTRQFYLWKLISIIKLCFIFIKELYLANVSVMKSVLS
PKLNIRPGIFAFKTELTKDWEITMLSLLITLTPGTLVMDISDDRTILYIHAMDIEDAEKAIFDIRESFEKAIQEVSR

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1863

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhE subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit E family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MRPE_BACSU (Q7WY60)

Other databases:

- EMBL:   AL009126
- RefSeq:   YP_054591.2
- EnsemblBacteria:   EBBACT00000003998
- GeneID:   2914191
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31640
- GenoList:   BSU31640
- GeneTree:   EBGT00050000002783
- HOGENOM:   HBG751150
- ProtClustDB:   PRK12651
- BioCyc:   BSUB:BSU31640-MONOMER
- InterPro:   IPR002758

Pfam domain/function: PF01899 MNHE

EC number: NA

Molecular weight: Translated: 18380; Mature: 18249

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x120a6074)-; HASH(0x10ca7354)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFQILLNVFLAFCWMFLSNSPSAAGFITGYILGMLSLFFFRRFFTRQFYLWKLISIIKL
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CFIFIKELYLANVSVMKSVLSPKLNIRPGIFAFKTELTKDWEITMLSLLITLTPGTLVMD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEE
ISDDRTILYIHAMDIEDAEKAIFDIRESFEKAIQEVSR
ECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
AFQILLNVFLAFCWMFLSNSPSAAGFITGYILGMLSLFFFRRFFTRQFYLWKLISIIKL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CFIFIKELYLANVSVMKSVLSPKLNIRPGIFAFKTELTKDWEITMLSLLITLTPGTLVMD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEE
ISDDRTILYIHAMDIEDAEKAIFDIRESFEKAIQEVSR
ECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 10198001; 11356194