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The map label for this gene is mrpD

Identifier: 255767720

GI number: 255767720

Start: 3249761

End: 3251242

Strand: Direct

Name: mrpD

Synonym: BSU31630

Alternate gene names: 255767720

Gene position: 3249761-3251242 (Clockwise)

Preceding gene: 255767719

Following gene: 255767721

Centisome position: 77.09

GC content: 46.83

Gene sequence:

>1482_bases
ATGAATAATTTTGTTATTTTGCCAATCCTGATCCCGCTTTTGTCGGCTATTCTTCTGATTTTCATGACGAAGAATCTTAT
GCTCATGAGAATCTTCAGCACGGCGGCATCGGCCATCGGGATTGTGATTAGCGGCATACTTGTACAGACTGTCTTCACAA
AAGGCATCCAGACACTCAGCCTAGGCGGATGGAAAGCGCCGTACGGCATTGTGCTTGCCGCAGACCAATTCGCCAGCCTT
CTCGTTCTCACAACAGCGATCATCGGTTTATTGGTTGGGCTTTATTCCTTCCGTTCTGTCGGTGAAAAACGGGAGCGCTC
CTTTTACTACTCCGGCGTTCAGTTCTTGCTTGCCGGCGTCAGCGGAGCGTTTTTAACAGGCGATTTATTTAATATGTACG
TATTTTTTGAGCTTCTGCTTATTGCTTCCTATATGCTGATTGTATTAGGCGGCACAAAAATTCAGCTGCGGGAATCCCTT
AAATATATTGTGTTTAATATTGTATCGTCTGCCCTGTTTGTCATAGGGGTCGGCTTTTTATACGCTGTAACCGGTACGTT
AAACATGGCGGATCTTAGCGTGAAAATCAGCGAATCAGGACAAACCGGGCTGATCACTGTGATTGGTGTCCTGCTGCTTT
TGGTATTTGGCATGAAGGGCGGAATCTTCCCCCTCTACTTTTGGCTTCCAGGCTCTTATTACGCGCCACCGGCGGCAATC
TCCGCGTTGTTCGGCGCTTTGCTGACAAAAGTCGGGTTATATGCGATTACGAGGGTTTTCACATTGATTTTTATTCACGA
TACAGCCTTTACCCATCAGTTGATGATTTGGCTGGCGGCGCTGACTGTGATTTTTGGTGTGATCGGTTCACTCGCTTATT
CGAATGTCATGAAAATTGTGATCTATAACATTATTACCGCTGTCGGCGTGATTTTGTTCGGTGTGGCAGTACACACCCCC
GCATCCATTCAGGGCGCGATCTACTATCTGATTCACGATATGCTGATTAAGGGCGCTTTGTTCATGCTGGCAGGTACGCT
GATCGCATTGACTGGAACCGCGAGCCTGCATAAAATGGGCGGGCTGATCAAACGCTATCCTGTTCTTGGATGGATGTTTT
TCATTTCCGCTATTTCTCTTGCAGGCATTCCGCCACTGAGCGGATTTGTCGGCAAATTTAAAATTGCAGAGGGCGGCTTC
GCAGAAGGTGAATTTACGATATCCATGCTGATTCTGCTTTCGAGTCTGCTTGTACTGTACTCTGTGCTGAGGATCTTTAT
ACATGCGTTTTGGGGCGAAGAAAAAGAAACGCCAAAACCAAATCATCGAACAGCTAAAGGACTTCTTTATCCGGCAGCTA
TTTTTCTCTTGCTGTCCCTTCTCTTCGGATTGGGTACAGAATGGGTGTCGCCTTACGTTGATCAAGCGGCCGAGACGCTG
CTGAATCCGGAAAAATATATCGAAGCTGTTCTGAAGGAGTAG

Upstream 100 bases:

>100_bases
ATTTCTTTTGGCGTTACGTCATTCATCCTCGTCATGGCCTTTCGCGCTTATCAGGAATTGAAATCGGACGATATGGATCA
AATGAGGGGAAATGATCAAC

Downstream 100 bases:

>100_bases
ATCGCATGGCATTTCAAATTTTATTAAATGTGTTTCTCGCTTTTTGCTGGATGTTCTTAAGCAACAGTCCAAGCGCCGCA
GGATTCATTACAGGCTATAT

Product: monovalent cation/H+ antiporter subunit D

Products: H2; oxidized ferredoxin [C]

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MNNFVILPILIPLLSAILLIFMTKNLMLMRIFSTAASAIGIVISGILVQTVFTKGIQTLSLGGWKAPYGIVLAADQFASL
LVLTTAIIGLLVGLYSFRSVGEKRERSFYYSGVQFLLAGVSGAFLTGDLFNMYVFFELLLIASYMLIVLGGTKIQLRESL
KYIVFNIVSSALFVIGVGFLYAVTGTLNMADLSVKISESGQTGLITVIGVLLLLVFGMKGGIFPLYFWLPGSYYAPPAAI
SALFGALLTKVGLYAITRVFTLIFIHDTAFTHQLMIWLAALTVIFGVIGSLAYSNVMKIVIYNIITAVGVILFGVAVHTP
ASIQGAIYYLIHDMLIKGALFMLAGTLIALTGTASLHKMGGLIKRYPVLGWMFFISAISLAGIPPLSGFVGKFKIAEGGF
AEGEFTISMLILLSSLLVLYSVLRIFIHAFWGEEKETPKPNHRTAKGLLYPAAIFLLLSLLFGLGTEWVSPYVDQAAETL
LNPEKYIEAVLKE

Sequences:

>Translated_493_residues
MNNFVILPILIPLLSAILLIFMTKNLMLMRIFSTAASAIGIVISGILVQTVFTKGIQTLSLGGWKAPYGIVLAADQFASL
LVLTTAIIGLLVGLYSFRSVGEKRERSFYYSGVQFLLAGVSGAFLTGDLFNMYVFFELLLIASYMLIVLGGTKIQLRESL
KYIVFNIVSSALFVIGVGFLYAVTGTLNMADLSVKISESGQTGLITVIGVLLLLVFGMKGGIFPLYFWLPGSYYAPPAAI
SALFGALLTKVGLYAITRVFTLIFIHDTAFTHQLMIWLAALTVIFGVIGSLAYSNVMKIVIYNIITAVGVILFGVAVHTP
ASIQGAIYYLIHDMLIKGALFMLAGTLIALTGTASLHKMGGLIKRYPVLGWMFFISAISLAGIPPLSGFVGKFKIAEGGF
AEGEFTISMLILLSSLLVLYSVLRIFIHAFWGEEKETPKPNHRTAKGLLYPAAIFLLLSLLFGLGTEWVSPYVDQAAETL
LNPEKYIEAVLKE
>Mature_493_residues
MNNFVILPILIPLLSAILLIFMTKNLMLMRIFSTAASAIGIVISGILVQTVFTKGIQTLSLGGWKAPYGIVLAADQFASL
LVLTTAIIGLLVGLYSFRSVGEKRERSFYYSGVQFLLAGVSGAFLTGDLFNMYVFFELLLIASYMLIVLGGTKIQLRESL
KYIVFNIVSSALFVIGVGFLYAVTGTLNMADLSVKISESGQTGLITVIGVLLLLVFGMKGGIFPLYFWLPGSYYAPPAAI
SALFGALLTKVGLYAITRVFTLIFIHDTAFTHQLMIWLAALTVIFGVIGSLAYSNVMKIVIYNIITAVGVILFGVAVHTP
ASIQGAIYYLIHDMLIKGALFMLAGTLIALTGTASLHKMGGLIKRYPVLGWMFFISAISLAGIPPLSGFVGKFKIAEGGF
AEGEFTISMLILLSSLLVLYSVLRIFIHAFWGEEKETPKPNHRTAKGLLYPAAIFLLLSLLFGLGTEWVSPYVDQAAETL
LNPEKYIEAVLKE

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family

Homologues:

Organism=Homo sapiens, GI251831117, Length=206, Percent_Identity=25.2427184466019, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI251831116, Length=350, Percent_Identity=22.2857142857143, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788827, Length=445, Percent_Identity=24.0449438202247, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788613, Length=353, Percent_Identity=26.9121813031161, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788829, Length=409, Percent_Identity=25.6723716381418, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788831, Length=437, Percent_Identity=24.7139588100687, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI2367154, Length=431, Percent_Identity=23.4338747099768, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788614, Length=482, Percent_Identity=23.8589211618257, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI145693160, Length=314, Percent_Identity=24.8407643312102, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MRPD_BACSU (O05229)

Other databases:

- EMBL:   Z93932
- EMBL:   AL009126
- PIR:   G70008
- RefSeq:   NP_391041.3
- EnsemblBacteria:   EBBACT00000002874
- GeneID:   938858
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31630
- GenoList:   BSU31630
- GeneTree:   EBGT00050000000597
- HOGENOM:   HBG643593
- PhylomeDB:   O05229
- ProtClustDB:   PRK07691
- BioCyc:   BSUB:BSU31630-MONOMER
- InterPro:   IPR004775
- InterPro:   IPR001750
- InterPro:   IPR003918
- PRINTS:   PR01437
- TIGRFAMs:   TIGR00944

Pfam domain/function: PF00361 Oxidored_q1

EC number: 1.-.-.- [C]

Molecular weight: Translated: 53478; Mature: 53478

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11221438)-; HASH(0x1222ac74)-; HASH(0x11fbc2e4)-; HASH(0x12226c74)-; HASH(0x12226db8)-; HASH(0x11f7ff38)-; HASH(0x12228a2c)-; HASH(0x12226fe0)-; HASH(0x1222d348)-; HASH(0x12228c24)-; HASH(0x11f7a9dc)-; HASH(0x11e3dc4c)-; HASH(0x1222ada0)-; HASH(0x121f1050)-; HASH(0x1222f120)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNFVILPILIPLLSAILLIFMTKNLMLMRIFSTAASAIGIVISGILVQTVFTKGIQTLS
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LGGWKAPYGIVLAADQFASLLVLTTAIIGLLVGLYSFRSVGEKRERSFYYSGVQFLLAGV
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGAFLTGDLFNMYVFFELLLIASYMLIVLGGTKIQLRESLKYIVFNIVSSALFVIGVGFL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAVTGTLNMADLSVKISESGQTGLITVIGVLLLLVFGMKGGIFPLYFWLPGSYYAPPAAI
HHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHH
SALFGALLTKVGLYAITRVFTLIFIHDTAFTHQLMIWLAALTVIFGVIGSLAYSNVMKIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYNIITAVGVILFGVAVHTPASIQGAIYYLIHDMLIKGALFMLAGTLIALTGTASLHKMG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GLIKRYPVLGWMFFISAISLAGIPPLSGFVGKFKIAEGGFAEGEFTISMLILLSSLLVLY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHH
SVLRIFIHAFWGEEKETPKPNHRTAKGLLYPAAIFLLLSLLFGLGTEWVSPYVDQAAETL
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LNPEKYIEAVLKE
CCHHHHHHHHHCH
>Mature Secondary Structure
MNNFVILPILIPLLSAILLIFMTKNLMLMRIFSTAASAIGIVISGILVQTVFTKGIQTLS
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LGGWKAPYGIVLAADQFASLLVLTTAIIGLLVGLYSFRSVGEKRERSFYYSGVQFLLAGV
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGAFLTGDLFNMYVFFELLLIASYMLIVLGGTKIQLRESLKYIVFNIVSSALFVIGVGFL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAVTGTLNMADLSVKISESGQTGLITVIGVLLLLVFGMKGGIFPLYFWLPGSYYAPPAAI
HHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHH
SALFGALLTKVGLYAITRVFTLIFIHDTAFTHQLMIWLAALTVIFGVIGSLAYSNVMKIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYNIITAVGVILFGVAVHTPASIQGAIYYLIHDMLIKGALFMLAGTLIALTGTASLHKMG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GLIKRYPVLGWMFFISAISLAGIPPLSGFVGKFKIAEGGFAEGEFTISMLILLSSLLVLY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHH
SVLRIFIHAFWGEEKETPKPNHRTAKGLLYPAAIFLLLSLLFGLGTEWVSPYVDQAAETL
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LNPEKYIEAVLKE
CCHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10568751; 10198001; 11356194