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The map label for this gene is mrpF

Identifier: 255767722

GI number: 255767722

Start: 3251724

End: 3252008

Strand: Direct

Name: mrpF

Synonym: BSU31650

Alternate gene names: 255767722

Gene position: 3251724-3252008 (Clockwise)

Preceding gene: 255767721

Following gene: 16080217

Centisome position: 77.14

GC content: 44.91

Gene sequence:

>285_bases
ATGTTTACGCTGATCCTGCAAATCGCGCTCGGCATTATGGCAGTGTCTACCTTTTTGTATGTCATTCGTGTCATTAAAGG
TCCGACTGTGCCTGACCGGGTCGTGGCTCTGGATGCAATCGGCATCAATCTGATCGCAATCACGGCGCTTGTCTCCATTT
TGCTGAAAACAAGTGCGTTTTTGGATATTATTTTGTTGCTGGGGATTTTATCGTTTATCGGAACGATCGCATTTTCCAAG
TTCCTGGAGAAAGGAGAGATTATCGAAAATGATCGAAACCGCTAA

Upstream 100 bases:

>100_bases
CAATACTTTATATTCATGCAATGGATATCGAGGATGCGGAAAAAGCGATTTTTGACATTCGGGAGTCATTTGAAAAAGCC
ATACAGGAGGTGAGCCGGTG

Downstream 100 bases:

>100_bases
GGTCGTTGTGGCTGTATTCATTTTGCTGGGTGCTCTTATATGCCTCATTGCTTCATTTGGCGTACTTCGCCTTCCCGATG
TGTTTACACGCGCTCATGCT

Product: monovalent cation/H+ antiporter subunit F

Products: NA

Alternate protein names: Mrp complex subunit F; Multiple resistance and pH homeostasis protein F; Sodium-cholate efflux protein MrpF

Number of amino acids: Translated: 94; Mature: 94

Protein sequence:

>94_residues
MFTLILQIALGIMAVSTFLYVIRVIKGPTVPDRVVALDAIGINLIAITALVSILLKTSAFLDIILLLGILSFIGTIAFSK
FLEKGEIIENDRNR

Sequences:

>Translated_94_residues
MFTLILQIALGIMAVSTFLYVIRVIKGPTVPDRVVALDAIGINLIAITALVSILLKTSAFLDIILLLGILSFIGTIAFSK
FLEKGEIIENDRNR
>Mature_94_residues
MFTLILQIALGIMAVSTFLYVIRVIKGPTVPDRVVALDAIGINLIAITALVSILLKTSAFLDIILLLGILSFIGTIAFSK
FLEKGEIIENDRNR

Specific function: Involved in cholate and Na(+) efflux activities, which may be mechanistically coupled. Does not require other mrp proteins for its own function

COG id: COG2212

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhF subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit F family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MRPF_BACSU (O05228)

Other databases:

- EMBL:   Z93932
- EMBL:   AL009126
- PIR:   F70008
- RefSeq:   NP_391043.2
- EnsemblBacteria:   EBBACT00000003622
- GeneID:   938862
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31650
- NMPDR:   fig|224308.1.peg.3168
- GenoList:   BSU31650
- GeneTree:   EBGT00050000000792
- HOGENOM:   HBG730934
- ProtClustDB:   PRK12600
- BioCyc:   BSUB:BSU31650-MONOMER
- InterPro:   IPR007208

Pfam domain/function: PF04066 MrpF_PhaF

EC number: NA

Molecular weight: Translated: 10245; Mature: 10245

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11b5622c)-; HASH(0x1198a254)-; HASH(0x11fce820)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTLILQIALGIMAVSTFLYVIRVIKGPTVPDRVVALDAIGINLIAITALVSILLKTSAF
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDIILLLGILSFIGTIAFSKFLEKGEIIENDRNR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
>Mature Secondary Structure
MFTLILQIALGIMAVSTFLYVIRVIKGPTVPDRVVALDAIGINLIAITALVSILLKTSAF
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDIILLLGILSFIGTIAFSKFLEKGEIIENDRNR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11004162; 11356194