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The map label for this gene is cca [H]

Identifier: 239617963

GI number: 239617963

Start: 1705420

End: 1708020

Strand: Reverse

Name: cca [H]

Synonym: Kole_1591

Alternate gene names: 239617963

Gene position: 1708020-1705420 (Counterclockwise)

Preceding gene: 239617964

Following gene: 239617962

Centisome position: 74.19

GC content: 42.25

Gene sequence:

>2601_bases
TTGAAAATTATAACGACGCATGTTAACCCTGATTTTGATGGGGTCGCTTCAGCGGTAGCGGCTCAGAGGCTTTTCCCTGA
CCACCAAATCGTTTTTGGTGGAAAACTTGATGAAGAATTACTTGATTTCATCGAAGAATTTTCTCCGTTTCTGTCCTTCA
GAATGGACGAAGACCTGACACTAACGAGTATTTCTTCCCTGGTAATCGTGGACAATTCGGATTATGATAGACTACCCGAA
AAACTCAAACCCTTCGTGAAAGAAGTCTCGACAATCGTATATGATCATCATGAAACAACAGCAACTCGCTGGGAAAAGCT
ATATGCAAAAGATCTTGGAGCGTGCATCACCCTTATTACGACGCACCTCATGGAAGCCGGTATAAAGATAACACCCGCTG
AAGCAACGTTGTTCCTAACGGCACTTTATCGGTCTACTCTCAACTTCACCCTGGCAACAACGACTCCCCAGGATCTGAAG
GTCGCATCATGGCTGCTGGAATCCGGAGCATCCTTTGAAGGAGTCTTAAAATACCTGAAGGTTAACCTTTCTGATGAACA
ACTAAAATTATACAGAAAACTGCTTGAAACTGTGAAAAAGCTTATCGTTGACGGAGTTGAAGTAGGAATCGCAACTGTTG
ATTATTCCACACTTCCGTCAGGCATATTCGAAATAGCAGACCGTATCTGGCGGTTTCTCGGTTACGATAACTTGATACTA
CTCATAAAATCAGGGAGACGCGTATTCATCATAGCTAGATCGCGATACAGTGAAATAGATTTTGGACGCATCTTTGAGGA
TGCGAATCTGCTATCACATCCTTTCTACACTCTGGGCTACTTGGACAACGTTTCCTTACAGGAGGTTGAAAAAAAGATAA
TCAGTTCATTGAAAGAGAATGTCCTGGAATTTGTTAGGGTTAAAGACATCATGTCATCGCCGGTGCGAACGGTTCTCGCG
GATATGCCCGTAAGTGAGGCTGCAAGGATAATGGCAAATACCGGTCATACCGGCCTCCCGGTAATAGATCACGGAAAACT
TGTGGGAATGATCGTGTGGAAGGATGTTCAAAAAGCATTGAAACACGGACTGGCCAACGAAAAAATAAGGGAAATCATGA
CAACGGAGCTCGTCACGGTGAAACCAACAGATTCAATCGGCGAAGCCATGAGAAAAATGGTTGAAAAAGGTGTGGGCAGA
ACACTGGTGGTGGAAAACGGAGTTCTTACCGGAATAGTTACCCGTAGCGACATAATGAGAAGCCGATACATATTGAAAGG
ACAACAGGATCGGTTTATTGGGTTCGCCACACCGATAACAACAAAGGTAGATAAACTGATCGAGGAAAGACTTCCAAGAA
GAATCCAGACATTGCTGCGCTTTCTTGGCGTCGTTGGAGACGAACTTTTGATGCCCACATATCTCGTTGGGGGAATGGTC
AGAGATCTTTTGCTGAATATACCAAATTACGATATCGATGTCGTTGTTGAAGGTGACGCCAGGATTTTCAGTCACACCGT
CAAAAGGTATTTCGATGTGAAAATCGTCGAACACAAGAGTTTTAAGACCTCCTCGATATTCTTCAGTGATGGTGTGAGAA
TAGACGTAGCAACGGCGAGAACTGAATTTTATGAAGAACCTGCTGTCCTTCCAAATGTTGAAACAAGCACAATAAAAAAG
GATTTGTACCGTAGAGACTTCTCGATAAATGCCATGGCAATAAAACTCAATCATGAAGAATATGGTCTTTTGCTTGATTT
CTTTGGTTGTAGAAAAGATCTCGCTAAAGGCATAATCCGCATTCTCCACAATCTGAGCTTTATTGAAGACCCAACAAGGA
TACTCAGAGCCATACGCTTCGAACAACGCTTCGGGTTCAAGATCGAAGAGAGAACGTTGCAACTGTTGAAAAAAGCTGTG
AATGATGGTTACCTTGAAAGAGTCACCGGTCAGAGACTGAGAGATGAGCTGGAGAAAATTCTTGCCGAACCTGAACCTCA
AAACGCAGTAAAAAGGATGACAAAACTTGATATTCTCAAACATTTGTTTCCGGGAGTTCGTCACAGCAAAGAGCTCCAGA
AACATTTTGACCGGCTGTTCACAAGATGGGAAACTATTGAGAAACTAACAAATGAAAGCAGAAAAATAACGGTTATGGTG
ATGCTGGTTCTCGAAAATACTCCAGAGGAATCAACAGACTGGATAATATCAAGATATGGTTTCCCCAAACGTCTTCCAGA
AGTTCTGAAAACTAGTCTAAGGATATTAAAAAAACTCCAAGAAATGGAGGACTGGAGATTTTCCAATCTATACAGACTGA
TGGAAAGACCATCGGCAGAGATTTTTCACTTTATCGATGCACATTTCGATGAAAAAAAGTCGAACATTCTGTTGAAATAT
CTCAAGGCTCTTGAAAGCAATAAACCAAAAGTAAATGGTGGAATTTTAATCCAGAAGTACGGCTTGAAAGAAGGCCCAGA
GATAAAAATGATACTGAAAGAATTATACTGTGCACGTCTTGAGGGCTTACCAGAAAGTGAGGAAGAGGCTTTTGTCAGAA
AGCTTCTAAAAGAACCCCCGGAAATGAGGGGAAAACGCTAA

Upstream 100 bases:

>100_bases
AGTTACTGAAAAAGTACTCCGGAAATAAAACGAAGGTCGCTCAAGTACTTGGAATCAGTAAGAGCACTCTCTACGAAAAA
CTCAAAAGGTGGGAACCCTT

Downstream 100 bases:

>100_bases
TCGAGGAGGTAAACAGTGGAAGAAAACGTTCTGGTAGTAAAAACACAGGCTCTTTCAGACATTCTCACGGAAGAAGGTGT
ACTTAAGGTAAGCGAAGAGA

Product: CBS domain containing protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 866; Mature: 866

Protein sequence:

>866_residues
MKIITTHVNPDFDGVASAVAAQRLFPDHQIVFGGKLDEELLDFIEEFSPFLSFRMDEDLTLTSISSLVIVDNSDYDRLPE
KLKPFVKEVSTIVYDHHETTATRWEKLYAKDLGACITLITTHLMEAGIKITPAEATLFLTALYRSTLNFTLATTTPQDLK
VASWLLESGASFEGVLKYLKVNLSDEQLKLYRKLLETVKKLIVDGVEVGIATVDYSTLPSGIFEIADRIWRFLGYDNLIL
LIKSGRRVFIIARSRYSEIDFGRIFEDANLLSHPFYTLGYLDNVSLQEVEKKIISSLKENVLEFVRVKDIMSSPVRTVLA
DMPVSEAARIMANTGHTGLPVIDHGKLVGMIVWKDVQKALKHGLANEKIREIMTTELVTVKPTDSIGEAMRKMVEKGVGR
TLVVENGVLTGIVTRSDIMRSRYILKGQQDRFIGFATPITTKVDKLIEERLPRRIQTLLRFLGVVGDELLMPTYLVGGMV
RDLLLNIPNYDIDVVVEGDARIFSHTVKRYFDVKIVEHKSFKTSSIFFSDGVRIDVATARTEFYEEPAVLPNVETSTIKK
DLYRRDFSINAMAIKLNHEEYGLLLDFFGCRKDLAKGIIRILHNLSFIEDPTRILRAIRFEQRFGFKIEERTLQLLKKAV
NDGYLERVTGQRLRDELEKILAEPEPQNAVKRMTKLDILKHLFPGVRHSKELQKHFDRLFTRWETIEKLTNESRKITVMV
MLVLENTPEESTDWIISRYGFPKRLPEVLKTSLRILKKLQEMEDWRFSNLYRLMERPSAEIFHFIDAHFDEKKSNILLKY
LKALESNKPKVNGGILIQKYGLKEGPEIKMILKELYCARLEGLPESEEEAFVRKLLKEPPEMRGKR

Sequences:

>Translated_866_residues
MKIITTHVNPDFDGVASAVAAQRLFPDHQIVFGGKLDEELLDFIEEFSPFLSFRMDEDLTLTSISSLVIVDNSDYDRLPE
KLKPFVKEVSTIVYDHHETTATRWEKLYAKDLGACITLITTHLMEAGIKITPAEATLFLTALYRSTLNFTLATTTPQDLK
VASWLLESGASFEGVLKYLKVNLSDEQLKLYRKLLETVKKLIVDGVEVGIATVDYSTLPSGIFEIADRIWRFLGYDNLIL
LIKSGRRVFIIARSRYSEIDFGRIFEDANLLSHPFYTLGYLDNVSLQEVEKKIISSLKENVLEFVRVKDIMSSPVRTVLA
DMPVSEAARIMANTGHTGLPVIDHGKLVGMIVWKDVQKALKHGLANEKIREIMTTELVTVKPTDSIGEAMRKMVEKGVGR
TLVVENGVLTGIVTRSDIMRSRYILKGQQDRFIGFATPITTKVDKLIEERLPRRIQTLLRFLGVVGDELLMPTYLVGGMV
RDLLLNIPNYDIDVVVEGDARIFSHTVKRYFDVKIVEHKSFKTSSIFFSDGVRIDVATARTEFYEEPAVLPNVETSTIKK
DLYRRDFSINAMAIKLNHEEYGLLLDFFGCRKDLAKGIIRILHNLSFIEDPTRILRAIRFEQRFGFKIEERTLQLLKKAV
NDGYLERVTGQRLRDELEKILAEPEPQNAVKRMTKLDILKHLFPGVRHSKELQKHFDRLFTRWETIEKLTNESRKITVMV
MLVLENTPEESTDWIISRYGFPKRLPEVLKTSLRILKKLQEMEDWRFSNLYRLMERPSAEIFHFIDAHFDEKKSNILLKY
LKALESNKPKVNGGILIQKYGLKEGPEIKMILKELYCARLEGLPESEEEAFVRKLLKEPPEMRGKR
>Mature_866_residues
MKIITTHVNPDFDGVASAVAAQRLFPDHQIVFGGKLDEELLDFIEEFSPFLSFRMDEDLTLTSISSLVIVDNSDYDRLPE
KLKPFVKEVSTIVYDHHETTATRWEKLYAKDLGACITLITTHLMEAGIKITPAEATLFLTALYRSTLNFTLATTTPQDLK
VASWLLESGASFEGVLKYLKVNLSDEQLKLYRKLLETVKKLIVDGVEVGIATVDYSTLPSGIFEIADRIWRFLGYDNLIL
LIKSGRRVFIIARSRYSEIDFGRIFEDANLLSHPFYTLGYLDNVSLQEVEKKIISSLKENVLEFVRVKDIMSSPVRTVLA
DMPVSEAARIMANTGHTGLPVIDHGKLVGMIVWKDVQKALKHGLANEKIREIMTTELVTVKPTDSIGEAMRKMVEKGVGR
TLVVENGVLTGIVTRSDIMRSRYILKGQQDRFIGFATPITTKVDKLIEERLPRRIQTLLRFLGVVGDELLMPTYLVGGMV
RDLLLNIPNYDIDVVVEGDARIFSHTVKRYFDVKIVEHKSFKTSSIFFSDGVRIDVATARTEFYEEPAVLPNVETSTIKK
DLYRRDFSINAMAIKLNHEEYGLLLDFFGCRKDLAKGIIRILHNLSFIEDPTRILRAIRFEQRFGFKIEERTLQLLKKAV
NDGYLERVTGQRLRDELEKILAEPEPQNAVKRMTKLDILKHLFPGVRHSKELQKHFDRLFTRWETIEKLTNESRKITVMV
MLVLENTPEESTDWIISRYGFPKRLPEVLKTSLRILKKLQEMEDWRFSNLYRLMERPSAEIFHFIDAHFDEKKSNILLKY
LKALESNKPKVNGGILIQKYGLKEGPEIKMILKELYCARLEGLPESEEEAFVRKLLKEPPEMRGKR

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=228, Percent_Identity=32.0175438596491, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI87081691, Length=228, Percent_Identity=27.1929824561404, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321016, Length=217, Percent_Identity=27.1889400921659, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 99462; Mature: 99462

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIITTHVNPDFDGVASAVAAQRLFPDHQIVFGGKLDEELLDFIEEFSPFLSFRMDEDLT
CEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCC
LTSISSLVIVDNSDYDRLPEKLKPFVKEVSTIVYDHHETTATRWEKLYAKDLGACITLIT
CEECCEEEEEECCCHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
THLMEAGIKITPAEATLFLTALYRSTLNFTLATTTPQDLKVASWLLESGASFEGVLKYLK
HHHHHCCCEECCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHH
VNLSDEQLKLYRKLLETVKKLIVDGVEVGIATVDYSTLPSGIFEIADRIWRFLGYDNLIL
CCCCHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEHHHCCHHHHHHHHHHHHHHCCCCEEE
LIKSGRRVFIIARSRYSEIDFGRIFEDANLLSHPFYTLGYLDNVSLQEVEKKIISSLKEN
EEECCCEEEEEECCCCCCCCHHHHHHHCHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHH
VLEFVRVKDIMSSPVRTVLADMPVSEAARIMANTGHTGLPVIDHGKLVGMIVWKDVQKAL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEECCCEEEHHHHHHHHHHH
KHGLANEKIREIMTTELVTVKPTDSIGEAMRKMVEKGVGRTLVVENGVLTGIVTRSDIMR
HCCCCHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEHHHHHHHH
SRYILKGQQDRFIGFATPITTKVDKLIEERLPRRIQTLLRFLGVVGDELLMPTYLVGGMV
HHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
RDLLLNIPNYDIDVVVEGDARIFSHTVKRYFDVKIVEHKSFKTSSIFFSDGVRIDVATAR
HHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCEEEEECCCCCCCEEEEECCCEEEEEHHH
TEFYEEPAVLPNVETSTIKKDLYRRDFSINAMAIKLNHEEYGLLLDFFGCRKDLAKGIIR
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
ILHNLSFIEDPTRILRAIRFEQRFGFKIEERTLQLLKKAVNDGYLERVTGQRLRDELEKI
HHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LAEPEPQNAVKRMTKLDILKHLFPGVRHSKELQKHFDRLFTRWETIEKLTNESRKITVMV
HCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
MLVLENTPEESTDWIISRYGFPKRLPEVLKTSLRILKKLQEMEDWRFSNLYRLMERPSAE
EEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHH
IFHFIDAHFDEKKSNILLKYLKALESNKPKVNGGILIQKYGLKEGPEIKMILKELYCARL
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
EGLPESEEEAFVRKLLKEPPEMRGKR
CCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKIITTHVNPDFDGVASAVAAQRLFPDHQIVFGGKLDEELLDFIEEFSPFLSFRMDEDLT
CEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCC
LTSISSLVIVDNSDYDRLPEKLKPFVKEVSTIVYDHHETTATRWEKLYAKDLGACITLIT
CEECCEEEEEECCCHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
THLMEAGIKITPAEATLFLTALYRSTLNFTLATTTPQDLKVASWLLESGASFEGVLKYLK
HHHHHCCCEECCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHH
VNLSDEQLKLYRKLLETVKKLIVDGVEVGIATVDYSTLPSGIFEIADRIWRFLGYDNLIL
CCCCHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEHHHCCHHHHHHHHHHHHHHCCCCEEE
LIKSGRRVFIIARSRYSEIDFGRIFEDANLLSHPFYTLGYLDNVSLQEVEKKIISSLKEN
EEECCCEEEEEECCCCCCCCHHHHHHHCHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHH
VLEFVRVKDIMSSPVRTVLADMPVSEAARIMANTGHTGLPVIDHGKLVGMIVWKDVQKAL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEECCCEEEHHHHHHHHHHH
KHGLANEKIREIMTTELVTVKPTDSIGEAMRKMVEKGVGRTLVVENGVLTGIVTRSDIMR
HCCCCHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEHHHHHHHH
SRYILKGQQDRFIGFATPITTKVDKLIEERLPRRIQTLLRFLGVVGDELLMPTYLVGGMV
HHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
RDLLLNIPNYDIDVVVEGDARIFSHTVKRYFDVKIVEHKSFKTSSIFFSDGVRIDVATAR
HHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCEEEEECCCCCCCEEEEECCCEEEEEHHH
TEFYEEPAVLPNVETSTIKKDLYRRDFSINAMAIKLNHEEYGLLLDFFGCRKDLAKGIIR
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
ILHNLSFIEDPTRILRAIRFEQRFGFKIEERTLQLLKKAVNDGYLERVTGQRLRDELEKI
HHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LAEPEPQNAVKRMTKLDILKHLFPGVRHSKELQKHFDRLFTRWETIEKLTNESRKITVMV
HCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
MLVLENTPEESTDWIISRYGFPKRLPEVLKTSLRILKKLQEMEDWRFSNLYRLMERPSAE
EEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHH
IFHFIDAHFDEKKSNILLKYLKALESNKPKVNGGILIQKYGLKEGPEIKMILKELYCARL
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
EGLPESEEEAFVRKLLKEPPEMRGKR
CCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA