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The map label for this gene is 239617069

Identifier: 239617069

GI number: 239617069

Start: 712860

End: 715190

Strand: Reverse

Name: 239617069

Synonym: Kole_0671

Alternate gene names: NA

Gene position: 715190-712860 (Counterclockwise)

Preceding gene: 239617070

Following gene: 239617068

Centisome position: 31.07

GC content: 35.52

Gene sequence:

>2331_bases
TTGTCTGAGGTTTATTCACACCCTCCAATGGGAAATGAACCTGGAAGATTATTGATCGACCATCTCAAAGGCGTATATTA
CCTGATGATGTCTGAAATAGAAACTGCAAAGAAATACATGGATTTTGAAAGGTTTCTTGGAGTTGAGTATTCAACCTTGA
AACTTCTCGTAGAAGCGATAGCATATTTGCATGATCTGGGTAAAGCAACACCGGAGTTTCAGAAAAAGATCCGGAAGTTA
AAATATGATAAAGAAAAATCTCTGCATGCTGTTCTGGGAGCATTTGCTGTTGGGAAATATTTGAAAGCAAATCTACCTGA
AGAGAAACATTATTTGATTCCTCTTATAGTAACTCTCGTAAGAAGACATCACGGAAAAGCAGAATATCCTGAATTTGGTT
GTGATCTAAATGATGAAGTCGATTTGTTGGAATGGCAGGTAGCCAATCTCGATTCTAAGTTTCTAAATGAACTTGCTTAT
AAAATCCAGATACCTAAGTTGGATCCTTTGGAACTTGAAGATAATGTAGATGAATGGCAGGATGAATTCCAGGATCATTT
CTACGAAGTTAATGACATTCAGTCTTTCATTCTTTACATGTATCTTTCATCCCTGCTTGATTGGGCAGACAAAACAGATG
CAGCGTTCAGGGATAGACCACTACCAAAAAGGGATCCAATTCCCAAAGACCTTGTTGAAGAGTATAGAAAAGTCTTGGGT
TTTGACAATCCTGGAAGAGATCCAATGAACCAGCTTAGAAACAAATTCTATCATGAAGCTACTAATGATACCGACTTCTC
CATAGGAATATTAAAAGGGAGAACAGGTATAGGCAAAACTTTGACTTTGTTTTCACTTGGGCTAAAACTCAGAGAAAAAC
TTACGAATAGTGATTATGTACCAAGAATAATCTATTGCCTTCCCTTTCTGAGCATAATAGATCAAACATATGAAGTAGCC
GTAGAACTTTTCGAAAAAACTGGTAGATCATTTCCAACTGAGAATCAGGTTTTACAGCAACATCACCTATCTGATATAGA
ATACTCCACTAAAGAAGGCGAAGGCTATGAGAAATATCTCGCCGATCTTCTTATAAATTCCTGGAATAGTGAGATAATTA
TAACTACTTTTGTCAGTTTCTTTCATTCTATTTTCACAAATAAACGAAACATGAAATTTTTTAGGATACCTGGGTCAATA
GTCTTGTTAGATGAAATTCAAGCTATACCTCCGAAGTATTGGGAACTGACAGGAAAAATATTGAAGCTTCTTGCTGATTA
TGGCGGAACTAAATTCGTATTCTCAACTGCTACTATGCCAAAGTGCTTTGGTATCTCTGGAAAACATCTCTATACTGGGG
AAATTCCTCTGGATAGATTTGATCTTCATTATATCGGAGAAGTAACTTTCGAAAAATTCAAGGAAATATTTCTTAGAGAG
GTCGTAGAAAAAGCCAGAGATGAGAGCAAAGGACTTATGGTGGTTTTAAATACAATTGGCTGTGCGAAAGCTGTACTGGA
AGAAATATCGGAATTCGTAGATAAAAAATATCTATATTATCTATCTAGCGAAGTTCCCCCGATAGTAAGAAAAAACCGAA
TTGAGAAGTTAAAAAATCTTAAAGGTTTTCGTGTATTAGTCACGACTCAACTTGTAGAAGCTGGTGTGGATCTGGATTTT
GATTATTGTATTCGTGATATTGGTCCTCTTGATAGTGTTGTTCAGGTTGCAGGAAGGGTGAACAGATCGAACAGGAAAAA
GAAAGGACAGGTAATTCTTGTAGATATAAAAGAATTCATGGGTAAAAGAGATCCAAGTAAAATATACGATTCGGTTTTGT
TGTTTGCTTCTAGAAAAGTACTTTCTAATAATGATAACTACAGTGAATCCATGCTTTATAATCTTGTTGAAATTTATCTT
GCAGAAATCGAAACCAAGGGATTTGAAGAAGAATCGCGAATGATACTCAAAAACATTTATAAGCTGGAATTTTCAAAAAT
TAATGAATTTAAGCTCATAGAAGAAAGACCAACGAACCCTGTTTTTATAGAACTCGACGAAAATGCAAAAAATGCCTGGA
AAAATTATCAGGAAATACTTTCAAAAGACATAAGCACACAGGACAAATTTGAGCTTCTAGCTGAAAAAAAAGATGCTATT
AGGAAACTTGCTCCTTACATAGTTAATTACCGATTGAAATGGAATGAGTCAGAAAATGCTTTGCCACCTGTTGTTAATGG
TTTCCACTATGTTTCTAACGACGAAAGGGAACTTCAAAGATACTATGACAGCGTAACTGGTTTTCATTCTGCAGGAAGTG
ATATTTATTGA

Upstream 100 bases:

>100_bases
GTATATGAAGAGAATGGTAAAAAGATACAACTGTTGAAAAAATCAGAGAAAACAAGTGTTATTTCTCTTGTCATCGGTGA
GCAAAAAGAGGTGATAATGC

Downstream 100 bases:

>100_bases
TAGACGTAAACGGTTATCTTATACTTTCATATTCAAACTGCCATCGCGAAGCATGGCTTGTAGCTCACAGAATTTTCCCT
GAAAATGATAATACTAATCT

Product: CRISPR-associated helicase Cas3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 776; Mature: 775

Protein sequence:

>776_residues
MSEVYSHPPMGNEPGRLLIDHLKGVYYLMMSEIETAKKYMDFERFLGVEYSTLKLLVEAIAYLHDLGKATPEFQKKIRKL
KYDKEKSLHAVLGAFAVGKYLKANLPEEKHYLIPLIVTLVRRHHGKAEYPEFGCDLNDEVDLLEWQVANLDSKFLNELAY
KIQIPKLDPLELEDNVDEWQDEFQDHFYEVNDIQSFILYMYLSSLLDWADKTDAAFRDRPLPKRDPIPKDLVEEYRKVLG
FDNPGRDPMNQLRNKFYHEATNDTDFSIGILKGRTGIGKTLTLFSLGLKLREKLTNSDYVPRIIYCLPFLSIIDQTYEVA
VELFEKTGRSFPTENQVLQQHHLSDIEYSTKEGEGYEKYLADLLINSWNSEIIITTFVSFFHSIFTNKRNMKFFRIPGSI
VLLDEIQAIPPKYWELTGKILKLLADYGGTKFVFSTATMPKCFGISGKHLYTGEIPLDRFDLHYIGEVTFEKFKEIFLRE
VVEKARDESKGLMVVLNTIGCAKAVLEEISEFVDKKYLYYLSSEVPPIVRKNRIEKLKNLKGFRVLVTTQLVEAGVDLDF
DYCIRDIGPLDSVVQVAGRVNRSNRKKKGQVILVDIKEFMGKRDPSKIYDSVLLFASRKVLSNNDNYSESMLYNLVEIYL
AEIETKGFEEESRMILKNIYKLEFSKINEFKLIEERPTNPVFIELDENAKNAWKNYQEILSKDISTQDKFELLAEKKDAI
RKLAPYIVNYRLKWNESENALPPVVNGFHYVSNDERELQRYYDSVTGFHSAGSDIY

Sequences:

>Translated_776_residues
MSEVYSHPPMGNEPGRLLIDHLKGVYYLMMSEIETAKKYMDFERFLGVEYSTLKLLVEAIAYLHDLGKATPEFQKKIRKL
KYDKEKSLHAVLGAFAVGKYLKANLPEEKHYLIPLIVTLVRRHHGKAEYPEFGCDLNDEVDLLEWQVANLDSKFLNELAY
KIQIPKLDPLELEDNVDEWQDEFQDHFYEVNDIQSFILYMYLSSLLDWADKTDAAFRDRPLPKRDPIPKDLVEEYRKVLG
FDNPGRDPMNQLRNKFYHEATNDTDFSIGILKGRTGIGKTLTLFSLGLKLREKLTNSDYVPRIIYCLPFLSIIDQTYEVA
VELFEKTGRSFPTENQVLQQHHLSDIEYSTKEGEGYEKYLADLLINSWNSEIIITTFVSFFHSIFTNKRNMKFFRIPGSI
VLLDEIQAIPPKYWELTGKILKLLADYGGTKFVFSTATMPKCFGISGKHLYTGEIPLDRFDLHYIGEVTFEKFKEIFLRE
VVEKARDESKGLMVVLNTIGCAKAVLEEISEFVDKKYLYYLSSEVPPIVRKNRIEKLKNLKGFRVLVTTQLVEAGVDLDF
DYCIRDIGPLDSVVQVAGRVNRSNRKKKGQVILVDIKEFMGKRDPSKIYDSVLLFASRKVLSNNDNYSESMLYNLVEIYL
AEIETKGFEEESRMILKNIYKLEFSKINEFKLIEERPTNPVFIELDENAKNAWKNYQEILSKDISTQDKFELLAEKKDAI
RKLAPYIVNYRLKWNESENALPPVVNGFHYVSNDERELQRYYDSVTGFHSAGSDIY
>Mature_775_residues
SEVYSHPPMGNEPGRLLIDHLKGVYYLMMSEIETAKKYMDFERFLGVEYSTLKLLVEAIAYLHDLGKATPEFQKKIRKLK
YDKEKSLHAVLGAFAVGKYLKANLPEEKHYLIPLIVTLVRRHHGKAEYPEFGCDLNDEVDLLEWQVANLDSKFLNELAYK
IQIPKLDPLELEDNVDEWQDEFQDHFYEVNDIQSFILYMYLSSLLDWADKTDAAFRDRPLPKRDPIPKDLVEEYRKVLGF
DNPGRDPMNQLRNKFYHEATNDTDFSIGILKGRTGIGKTLTLFSLGLKLREKLTNSDYVPRIIYCLPFLSIIDQTYEVAV
ELFEKTGRSFPTENQVLQQHHLSDIEYSTKEGEGYEKYLADLLINSWNSEIIITTFVSFFHSIFTNKRNMKFFRIPGSIV
LLDEIQAIPPKYWELTGKILKLLADYGGTKFVFSTATMPKCFGISGKHLYTGEIPLDRFDLHYIGEVTFEKFKEIFLREV
VEKARDESKGLMVVLNTIGCAKAVLEEISEFVDKKYLYYLSSEVPPIVRKNRIEKLKNLKGFRVLVTTQLVEAGVDLDFD
YCIRDIGPLDSVVQVAGRVNRSNRKKKGQVILVDIKEFMGKRDPSKIYDSVLLFASRKVLSNNDNYSESMLYNLVEIYLA
EIETKGFEEESRMILKNIYKLEFSKINEFKLIEERPTNPVFIELDENAKNAWKNYQEILSKDISTQDKFELLAEKKDAIR
KLAPYIVNYRLKWNESENALPPVVNGFHYVSNDERELQRYYDSVTGFHSAGSDIY

Specific function: Unknown

COG id: COG1203

COG function: function code R; Predicted helicases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR006474
- InterPro:   IPR014021 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 90158; Mature: 90027

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVYSHPPMGNEPGRLLIDHLKGVYYLMMSEIETAKKYMDFERFLGVEYSTLKLLVEAI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AYLHDLGKATPEFQKKIRKLKYDKEKSLHAVLGAFAVGKYLKANLPEEKHYLIPLIVTLV
HHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RRHHGKAEYPEFGCDLNDEVDLLEWQVANLDSKFLNELAYKIQIPKLDPLELEDNVDEWQ
HHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHEEECCCCCCCCCCCCHHHHH
DEFQDHFYEVNDIQSFILYMYLSSLLDWADKTDAAFRDRPLPKRDPIPKDLVEEYRKVLG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHC
FDNPGRDPMNQLRNKFYHEATNDTDFSIGILKGRTGIGKTLTLFSLGLKLREKLTNSDYV
CCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCH
PRIIYCLPFLSIIDQTYEVAVELFEKTGRSFPTENQVLQQHHLSDIEYSTKEGEGYEKYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHH
ADLLINSWNSEIIITTFVSFFHSIFTNKRNMKFFRIPGSIVLLDEIQAIPPKYWELTGKI
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCHHCCCCHHHHHHHHH
LKLLADYGGTKFVFSTATMPKCFGISGKHLYTGEIPLDRFDLHYIGEVTFEKFKEIFLRE
HHHHHHCCCCEEEEECCCCCHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
VVEKARDESKGLMVVLNTIGCAKAVLEEISEFVDKKYLYYLSSEVPPIVRKNRIEKLKNL
HHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
KGFRVLVTTQLVEAGVDLDFDYCIRDIGPLDSVVQVAGRVNRSNRKKKGQVILVDIKEFM
CCCEEHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHC
GKRDPSKIYDSVLLFASRKVLSNNDNYSESMLYNLVEIYLAEIETKGFEEESRMILKNIY
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KLEFSKINEFKLIEERPTNPVFIELDENAKNAWKNYQEILSKDISTQDKFELLAEKKDAI
HHHHHHCCCEEEHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RKLAPYIVNYRLKWNESENALPPVVNGFHYVSNDERELQRYYDSVTGFHSAGSDIY
HHHHHHHHEEEEEECCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHCHHCCCCCCC
>Mature Secondary Structure 
SEVYSHPPMGNEPGRLLIDHLKGVYYLMMSEIETAKKYMDFERFLGVEYSTLKLLVEAI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AYLHDLGKATPEFQKKIRKLKYDKEKSLHAVLGAFAVGKYLKANLPEEKHYLIPLIVTLV
HHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RRHHGKAEYPEFGCDLNDEVDLLEWQVANLDSKFLNELAYKIQIPKLDPLELEDNVDEWQ
HHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHEEECCCCCCCCCCCCHHHHH
DEFQDHFYEVNDIQSFILYMYLSSLLDWADKTDAAFRDRPLPKRDPIPKDLVEEYRKVLG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHC
FDNPGRDPMNQLRNKFYHEATNDTDFSIGILKGRTGIGKTLTLFSLGLKLREKLTNSDYV
CCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCH
PRIIYCLPFLSIIDQTYEVAVELFEKTGRSFPTENQVLQQHHLSDIEYSTKEGEGYEKYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHH
ADLLINSWNSEIIITTFVSFFHSIFTNKRNMKFFRIPGSIVLLDEIQAIPPKYWELTGKI
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCHHCCCCHHHHHHHHH
LKLLADYGGTKFVFSTATMPKCFGISGKHLYTGEIPLDRFDLHYIGEVTFEKFKEIFLRE
HHHHHHCCCCEEEEECCCCCHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
VVEKARDESKGLMVVLNTIGCAKAVLEEISEFVDKKYLYYLSSEVPPIVRKNRIEKLKNL
HHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
KGFRVLVTTQLVEAGVDLDFDYCIRDIGPLDSVVQVAGRVNRSNRKKKGQVILVDIKEFM
CCCEEHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHC
GKRDPSKIYDSVLLFASRKVLSNNDNYSESMLYNLVEIYLAEIETKGFEEESRMILKNIY
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KLEFSKINEFKLIEERPTNPVFIELDENAKNAWKNYQEILSKDISTQDKFELLAEKKDAI
HHHHHHCCCEEEHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RKLAPYIVNYRLKWNESENALPPVVNGFHYVSNDERELQRYYDSVTGFHSAGSDIY
HHHHHHHHEEEEEECCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]