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The map label for this gene is malZ [C]

Identifier: 239616594

GI number: 239616594

Start: 208020

End: 209510

Strand: Direct

Name: malZ [C]

Synonym: Kole_0186

Alternate gene names: 239616594

Gene position: 208020-209510 (Clockwise)

Preceding gene: 239616593

Following gene: 239616595

Centisome position: 9.04

GC content: 43.8

Gene sequence:

>1491_bases
ATGTTTGATTGGCATACTTCCAAAGTTGTTTATCAGATTTTCCCGGATAGATTCAATATAGGCGATGGAAAAACAGCCAA
ACAAAAGGAAAATGAAGGCCTCTATCCGCTTCCAGGCCAGAGAACGGTTGATTGGAAGGAAAAACCAAAAAGAACCGGTG
ACAGAACCCAGCAGCTTCTATTTTGGGGTGGAGACTTAAAAGGAATAACAGAAAAAATCGATTACATAAAGTCGCTTGGA
GCCGAAATAATTTATCTCACTCCCATCTTCTATGCCCATACGAACCACAAATACGACACCATAGATTTTTTCAGGATAGA
CCCTGCCTTCGGAACACTCCAGGATTTCAAGGAACTCTGCGAAACCGCTCACCAGAAGGATTTAAAAATAATCCTCGACG
GTGTTTTCAACCATGTGGGGTTTGCTTCAAAATGGTTCAACGGATTGAACCTGTTTCCTGAAAAAGGAGCCTACCAATCC
CCGAATTCTCCATATCGGGATTATTTCTACTTTTCGGGAGATAAATATAGGAGCTGGGTAAACTCCGGTTCTTTGCCAGA
ACTCAACCTTGATAACCCGGAACTCCAGGAGATCCTGTTTACCGGTAAAAACTCGGTGGTAAAGTACTGGCTAAAGCAGG
GTGCAGATGGCTGGCGAATTGATTGTGCACACGATATAGGAAATCTCAACCACATAATAGTTGAAGCAGCAAAAAGTGTT
AAACCTGATGCACTCATAATTGGTGAGGTCTGGAATTATCCAAAGGGCTGGAACGAATTCGCAAGGCTCGATGGCATAAT
GAACTACCATTTCAGGTCCCTGGTGATAGATTTGCTGAAAGCGGACATGCCGGTTGAAACAGTAGCGAAAATAATGAAAG
ATACCGTTGAGGACTGCGGTATTGATTATCTCTCAAAGTGCTGGAATATGCTCTCCTCACACGATGTCCCGCGTTTGTCA
AATCTTTTCGACAACGAAAGAGATATTCGACTCGCTATCGCTCTCCAATTCAGCTTTCCAGGAGTTCCAATGATCTACTA
CGGAGAAGAGCTTGAGATGACCGGCGGAGAGGATCCTGAAAACCGCGATGCGATGCGATGGGAAAATCTGAGCAACTCAC
CAGAACGGCTTAACTTCTACAAGAAATTGACTGAAATACGCAAGAAAAAAATTGCCCTACAAATAGGAAGCTTTGGCATT
CTCTATACCTCGCAACCCGACAGGACAATCGTATTCAAACGCTCGACGGAAAGAATAGACGATCTGCTACTGATAGCTTT
TAATCTTTCCTTTGAAAAGGTTCAATTCAAAACCTACATAAATGAAAGCACTTTAATGACGGGAACTCTTTTCAGGGATC
TGTTCACCGGCGAAACGTTCCGAACGGACGTGTCGAACATCACCCTCCAGGTTGAACCACGATCCTTCAGAATTCTTGAG
CCCATTATCGAAAGAGTCCCGGACAGGTACACACCATATAAGAGGGTGTAG

Upstream 100 bases:

>100_bases
TCGATATGACCGTAGATGATCTGTGGTCAATAAGGAACAATATTTTTCTGGGAATGGTGTACTTGGCGTACGCCCAAACT
TTGATCGAAAGGTAGATATT

Downstream 100 bases:

>100_bases
CACTGTGAATAAAAGAGTTACCGCAACACTCCTGCTGGCAACTACTGCCATCATCTGGGGAGAATCATATCTTTTTACAA
AGGTGGCTGTAGAAAGCATG

Product: alpha amylase catalytic region

Products: NA

Alternate protein names: CDase; Cyclomaltodextrin hydrolase, decycling [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MFDWHTSKVVYQIFPDRFNIGDGKTAKQKENEGLYPLPGQRTVDWKEKPKRTGDRTQQLLFWGGDLKGITEKIDYIKSLG
AEIIYLTPIFYAHTNHKYDTIDFFRIDPAFGTLQDFKELCETAHQKDLKIILDGVFNHVGFASKWFNGLNLFPEKGAYQS
PNSPYRDYFYFSGDKYRSWVNSGSLPELNLDNPELQEILFTGKNSVVKYWLKQGADGWRIDCAHDIGNLNHIIVEAAKSV
KPDALIIGEVWNYPKGWNEFARLDGIMNYHFRSLVIDLLKADMPVETVAKIMKDTVEDCGIDYLSKCWNMLSSHDVPRLS
NLFDNERDIRLAIALQFSFPGVPMIYYGEELEMTGGEDPENRDAMRWENLSNSPERLNFYKKLTEIRKKKIALQIGSFGI
LYTSQPDRTIVFKRSTERIDDLLLIAFNLSFEKVQFKTYINESTLMTGTLFRDLFTGETFRTDVSNITLQVEPRSFRILE
PIIERVPDRYTPYKRV

Sequences:

>Translated_496_residues
MFDWHTSKVVYQIFPDRFNIGDGKTAKQKENEGLYPLPGQRTVDWKEKPKRTGDRTQQLLFWGGDLKGITEKIDYIKSLG
AEIIYLTPIFYAHTNHKYDTIDFFRIDPAFGTLQDFKELCETAHQKDLKIILDGVFNHVGFASKWFNGLNLFPEKGAYQS
PNSPYRDYFYFSGDKYRSWVNSGSLPELNLDNPELQEILFTGKNSVVKYWLKQGADGWRIDCAHDIGNLNHIIVEAAKSV
KPDALIIGEVWNYPKGWNEFARLDGIMNYHFRSLVIDLLKADMPVETVAKIMKDTVEDCGIDYLSKCWNMLSSHDVPRLS
NLFDNERDIRLAIALQFSFPGVPMIYYGEELEMTGGEDPENRDAMRWENLSNSPERLNFYKKLTEIRKKKIALQIGSFGI
LYTSQPDRTIVFKRSTERIDDLLLIAFNLSFEKVQFKTYINESTLMTGTLFRDLFTGETFRTDVSNITLQVEPRSFRILE
PIIERVPDRYTPYKRV
>Mature_496_residues
MFDWHTSKVVYQIFPDRFNIGDGKTAKQKENEGLYPLPGQRTVDWKEKPKRTGDRTQQLLFWGGDLKGITEKIDYIKSLG
AEIIYLTPIFYAHTNHKYDTIDFFRIDPAFGTLQDFKELCETAHQKDLKIILDGVFNHVGFASKWFNGLNLFPEKGAYQS
PNSPYRDYFYFSGDKYRSWVNSGSLPELNLDNPELQEILFTGKNSVVKYWLKQGADGWRIDCAHDIGNLNHIIVEAAKSV
KPDALIIGEVWNYPKGWNEFARLDGIMNYHFRSLVIDLLKADMPVETVAKIMKDTVEDCGIDYLSKCWNMLSSHDVPRLS
NLFDNERDIRLAIALQFSFPGVPMIYYGEELEMTGGEDPENRDAMRWENLSNSPERLNFYKKLTEIRKKKIALQIGSFGI
LYTSQPDRTIVFKRSTERIDDLLLIAFNLSFEKVQFKTYINESTLMTGTLFRDLFTGETFRTDVSNITLQVEPRSFRILE
PIIERVPDRYTPYKRV

Specific function: Hydrolyzes cyclodextrins. Can also act on linear maltodextrins, with the exception of maltose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=473, Percent_Identity=22.1987315010571, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1786604, Length=452, Percent_Identity=33.4070796460177, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1790687, Length=349, Percent_Identity=26.0744985673352, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789995, Length=507, Percent_Identity=23.4714003944773, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI32565753, Length=402, Percent_Identity=23.6318407960199, Blast_Score=89, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6322245, Length=239, Percent_Identity=28.8702928870293, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6324416, Length=218, Percent_Identity=28.4403669724771, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322241, Length=218, Percent_Identity=27.9816513761468, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322021, Length=218, Percent_Identity=27.9816513761468, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321726, Length=216, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6321731, Length=192, Percent_Identity=28.6458333333333, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319776, Length=192, Percent_Identity=28.6458333333333, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24586599, Length=547, Percent_Identity=24.6800731261426, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24586593, Length=412, Percent_Identity=24.7572815533981, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24586587, Length=387, Percent_Identity=26.8733850129199, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24583747, Length=400, Percent_Identity=24.75, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583749, Length=400, Percent_Identity=24.75, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24586589, Length=190, Percent_Identity=31.5789473684211, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24586591, Length=192, Percent_Identity=30.7291666666667, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24586597, Length=191, Percent_Identity=32.9842931937173, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI221330053, Length=192, Percent_Identity=32.2916666666667, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI45549022, Length=369, Percent_Identity=24.9322493224932, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583745, Length=393, Percent_Identity=22.9007633587786, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.54 [H]

Molecular weight: Translated: 57615; Mature: 57615

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDWHTSKVVYQIFPDRFNIGDGKTAKQKENEGLYPLPGQRTVDWKEKPKRTGDRTQQLL
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHEEE
FWGGDLKGITEKIDYIKSLGAEIIYLTPIFYAHTNHKYDTIDFFRIDPAFGTLQDFKELC
EECCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHH
ETAHQKDLKIILDGVFNHVGFASKWFNGLNLFPEKGAYQSPNSPYRDYFYFSGDKYRSWV
HHHHHCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHH
NSGSLPELNLDNPELQEILFTGKNSVVKYWLKQGADGWRIDCAHDIGNLNHIIVEAAKSV
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEHHHCCCHHHHHHHHHHCC
KPDALIIGEVWNYPKGWNEFARLDGIMNYHFRSLVIDLLKADMPVETVAKIMKDTVEDCG
CCCEEEEECHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
IDYLSKCWNMLSSHDVPRLSNLFDNERDIRLAIALQFSFPGVPMIYYGEELEMTGGEDPE
HHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEEEEECCCCCEEEEECCEEEECCCCCCC
NRDAMRWENLSNSPERLNFYKKLTEIRKKKIALQIGSFGILYTSQPDRTIVFKRSTERID
CCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCCCCEEEEECCHHHHH
DLLLIAFNLSFEKVQFKTYINESTLMTGTLFRDLFTGETFRTDVSNITLQVEPRSFRILE
HEEEEEEECCHHHEEEEECCCCCHHHHHHHHHHHHCCCHHEECCCEEEEEECCCCHHHHH
PIIERVPDRYTPYKRV
HHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFDWHTSKVVYQIFPDRFNIGDGKTAKQKENEGLYPLPGQRTVDWKEKPKRTGDRTQQLL
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHEEE
FWGGDLKGITEKIDYIKSLGAEIIYLTPIFYAHTNHKYDTIDFFRIDPAFGTLQDFKELC
EECCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHH
ETAHQKDLKIILDGVFNHVGFASKWFNGLNLFPEKGAYQSPNSPYRDYFYFSGDKYRSWV
HHHHHCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHH
NSGSLPELNLDNPELQEILFTGKNSVVKYWLKQGADGWRIDCAHDIGNLNHIIVEAAKSV
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEHHHCCCHHHHHHHHHHCC
KPDALIIGEVWNYPKGWNEFARLDGIMNYHFRSLVIDLLKADMPVETVAKIMKDTVEDCG
CCCEEEEECHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
IDYLSKCWNMLSSHDVPRLSNLFDNERDIRLAIALQFSFPGVPMIYYGEELEMTGGEDPE
HHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEEEEECCCCCEEEEECCEEEECCCCCCC
NRDAMRWENLSNSPERLNFYKKLTEIRKKKIALQIGSFGILYTSQPDRTIVFKRSTERID
CCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCCCCEEEEECCHHHHH
DLLLIAFNLSFEKVQFKTYINESTLMTGTLFRDLFTGETFRTDVSNITLQVEPRSFRILE
HEEEEEEECCHHHEEEEECCCCCHHHHHHHHHHHHCCCHHEECCCEEEEEECCCCHHHHH
PIIERVPDRYTPYKRV
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644767; 8425614 [H]