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The map label for this gene is pcrA [H]

Identifier: 239616541

GI number: 239616541

Start: 148036

End: 149994

Strand: Direct

Name: pcrA [H]

Synonym: Kole_0133

Alternate gene names: 239616541

Gene position: 148036-149994 (Clockwise)

Preceding gene: 239616535

Following gene: 239616542

Centisome position: 6.43

GC content: 44.67

Gene sequence:

>1959_bases
ATGAAGCGCTATAATCTTAAACCACATGGTATACCAAACTTTATCCAGGAATCTCTTGACAACGAACAATTAAACGCCGT
TCTCAATTCTAAAGGGAGAACGCTTATCGTTGCAGGACCTGGCTCCGGTAAAACCAGGGTCATCACGTTCAAGATCGCTT
ACCTTGTCTCTTCAGGGGTGAATCCTCAAAATATCCTTCTGGTCACGTTTACAAGGGCTGCTTCCAGAGAAATGATAGAA
AGAGCAAGAAGAGTCAGCGGTTCTGACCTTCGGGGAATGCTTGCGGGAACTTTTCATCACGTTTGCAATCATTTTCTTCG
GAAATATGCCACCCACTTAGGTATCTCATCTGATTTTACAATCCTCGACAGAGAAGACTCCAAGGATTTAATAAAACACT
GCAAAGCCCAATTGATGGAACAGCGTGGAAATGATGGTTCCCTCAAGCTTCCAAGCCCGGGAGTTATCCAGAGTATCTTC
TCCTATTCCGTAAACTGCATGGTTTCACTTGACGAATCAATAGCAAAGCACAACCGTAAGTTCTTCGCGGTTCGGGATGA
GCTCGAGGAGATATGGAAAAGCTACACTCAACTGAAGTTAGAACACAACACCCTTGATTACGATGACCTTTTGATAAAAG
CCGTTGAATTGTTTGAGAATAACCCAGAAATCTTAATTCGTGAATCTTCAAGATTCCAGTGGATTCTGGTAGATGAATTC
CAGGACACCAATCTCGTACAGTACAAACTCGTTGAGATGCTTGCGGGTGTACACGGCAATCTCATAGTCGTCGGGGATGA
CGCCCAATCAATATACTCTTTCCGTGGCGCAAGGTTCGAAAACGTGTACGACTTCCTGAACCGTGACCAAACCTCCGTGT
TCAAGATTCAAACCAATTATCGTTCAACACCGGAGATCGTCAGGCTGGTAAATCATCTTGTCCCAAAAAGCTCCCTTGAA
AAACAATTGAAAGCTGTTCGGCCTTCAGGCCCGGTTCCAGTGATCGTGGAAACCTGGGACAATCTTGATGAAGCTTCCTT
CGTGGCTCAAAAAATCAATGAGCATATTGAAGACGGCATTCAACCAAACAGGATAGCTGTGCTGTACAGATCTCATTTCC
ATTCCTTGGAGCTACAACTTGAATTAGACAGGAGAAGAGTCCCTTACATAGTTTTCTCAGGCCCAAAGTTCGTTGAAACC
GCTCACGTTAAAGACATCCTCGCTTTCCTTAAGATCCTTCAGAATCCATACGATCAATTATCCTGGGGAAGGGTATTGAG
GCTTTTCCCCGGCGTTGGTATACGTAGAGCAACCCAGATCATAAATGCTATTAGAGAGTCTTTAGGAAACGACAACGAGG
TTATTTCCGCTCTCAGGCCATTCGCAACTCCCCGGGTACGGATAGAAAGATTGATTTCACTTTTAGAAGAGCTCAACAGT
GAGCAAAGCCCGGAAGAAGTGATTTCAACTATTTATAAAGGCTTTTATGGTGAATATATGGACGAGCATTTTTCTGACGC
CAAAGAAAGGCGTATGGATATCGAAAGGCTTATAGAAGTTGCAGATCGTTACGATAGTATAGGAGAATTCCTCGAAGATC
TCGCCGTCAGCGAAAAGGTTGATATAGAAAGAGAAATAGTTGACAGAGAAGAGAAAATCGTGTTAAGCACCGTTCATCAG
GCAAAAGGGCTTGAGTGGGATGTTGTTTTCATTCTCGCCATGAATCCGGGAGATTTCCCGCATGCTCTTGCAATAATGGA
AGGAAACCTCGATGAGGAGGAGCGAATCTTCTATGTGGCTGTCACAAGGGCCCGGGATTATCTTTACCTCATACGCCAGA
GAACCGGCAGGTCGCGCCCAATGATCGGAAATTCCTACGTCTTCAGGAGCGGACATGACTTTCTTACAAAGATCCCGGAG
GATGTGGCTGAAAGATGGGACGTTGGGTGGAATATTTAA

Upstream 100 bases:

>100_bases
TGAAATTTCTTCCATTTTATCCCTCCAGAGAAAATAATAACAGAAATCGCGTAAAACAGTTAGCTCCGGATTGTGATAAA
ATTTTGAAGAGGAGGAAGCT

Downstream 100 bases:

>100_bases
TACCATATTTCTCTTGATTCCTATGAAGGAGGGGAAAAAATGAATGCTCTGTATCTCCCACTAACTGCGGGTGTTCTTTC
GACCATCTTTGCATTTATCA

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MKRYNLKPHGIPNFIQESLDNEQLNAVLNSKGRTLIVAGPGSGKTRVITFKIAYLVSSGVNPQNILLVTFTRAASREMIE
RARRVSGSDLRGMLAGTFHHVCNHFLRKYATHLGISSDFTILDREDSKDLIKHCKAQLMEQRGNDGSLKLPSPGVIQSIF
SYSVNCMVSLDESIAKHNRKFFAVRDELEEIWKSYTQLKLEHNTLDYDDLLIKAVELFENNPEILIRESSRFQWILVDEF
QDTNLVQYKLVEMLAGVHGNLIVVGDDAQSIYSFRGARFENVYDFLNRDQTSVFKIQTNYRSTPEIVRLVNHLVPKSSLE
KQLKAVRPSGPVPVIVETWDNLDEASFVAQKINEHIEDGIQPNRIAVLYRSHFHSLELQLELDRRRVPYIVFSGPKFVET
AHVKDILAFLKILQNPYDQLSWGRVLRLFPGVGIRRATQIINAIRESLGNDNEVISALRPFATPRVRIERLISLLEELNS
EQSPEEVISTIYKGFYGEYMDEHFSDAKERRMDIERLIEVADRYDSIGEFLEDLAVSEKVDIEREIVDREEKIVLSTVHQ
AKGLEWDVVFILAMNPGDFPHALAIMEGNLDEEERIFYVAVTRARDYLYLIRQRTGRSRPMIGNSYVFRSGHDFLTKIPE
DVAERWDVGWNI

Sequences:

>Translated_652_residues
MKRYNLKPHGIPNFIQESLDNEQLNAVLNSKGRTLIVAGPGSGKTRVITFKIAYLVSSGVNPQNILLVTFTRAASREMIE
RARRVSGSDLRGMLAGTFHHVCNHFLRKYATHLGISSDFTILDREDSKDLIKHCKAQLMEQRGNDGSLKLPSPGVIQSIF
SYSVNCMVSLDESIAKHNRKFFAVRDELEEIWKSYTQLKLEHNTLDYDDLLIKAVELFENNPEILIRESSRFQWILVDEF
QDTNLVQYKLVEMLAGVHGNLIVVGDDAQSIYSFRGARFENVYDFLNRDQTSVFKIQTNYRSTPEIVRLVNHLVPKSSLE
KQLKAVRPSGPVPVIVETWDNLDEASFVAQKINEHIEDGIQPNRIAVLYRSHFHSLELQLELDRRRVPYIVFSGPKFVET
AHVKDILAFLKILQNPYDQLSWGRVLRLFPGVGIRRATQIINAIRESLGNDNEVISALRPFATPRVRIERLISLLEELNS
EQSPEEVISTIYKGFYGEYMDEHFSDAKERRMDIERLIEVADRYDSIGEFLEDLAVSEKVDIEREIVDREEKIVLSTVHQ
AKGLEWDVVFILAMNPGDFPHALAIMEGNLDEEERIFYVAVTRARDYLYLIRQRTGRSRPMIGNSYVFRSGHDFLTKIPE
DVAERWDVGWNI
>Mature_652_residues
MKRYNLKPHGIPNFIQESLDNEQLNAVLNSKGRTLIVAGPGSGKTRVITFKIAYLVSSGVNPQNILLVTFTRAASREMIE
RARRVSGSDLRGMLAGTFHHVCNHFLRKYATHLGISSDFTILDREDSKDLIKHCKAQLMEQRGNDGSLKLPSPGVIQSIF
SYSVNCMVSLDESIAKHNRKFFAVRDELEEIWKSYTQLKLEHNTLDYDDLLIKAVELFENNPEILIRESSRFQWILVDEF
QDTNLVQYKLVEMLAGVHGNLIVVGDDAQSIYSFRGARFENVYDFLNRDQTSVFKIQTNYRSTPEIVRLVNHLVPKSSLE
KQLKAVRPSGPVPVIVETWDNLDEASFVAQKINEHIEDGIQPNRIAVLYRSHFHSLELQLELDRRRVPYIVFSGPKFVET
AHVKDILAFLKILQNPYDQLSWGRVLRLFPGVGIRRATQIINAIRESLGNDNEVISALRPFATPRVRIERLISLLEELNS
EQSPEEVISTIYKGFYGEYMDEHFSDAKERRMDIERLIEVADRYDSIGEFLEDLAVSEKVDIEREIVDREEKIVLSTVHQ
AKGLEWDVVFILAMNPGDFPHALAIMEGNLDEEERIFYVAVTRARDYLYLIRQRTGRSRPMIGNSYVFRSGHDFLTKIPE
DVAERWDVGWNI

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=666, Percent_Identity=31.6816816816817, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI48994965, Length=623, Percent_Identity=30.1765650080257, Blast_Score=222, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6322369, Length=486, Percent_Identity=27.3662551440329, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324477, Length=426, Percent_Identity=25.8215962441315, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 75002; Mature: 75002

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYNLKPHGIPNFIQESLDNEQLNAVLNSKGRTLIVAGPGSGKTRVITFKIAYLVSSGV
CCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEEEHHHHCCC
NPQNILLVTFTRAASREMIERARRVSGSDLRGMLAGTFHHVCNHFLRKYATHLGISSDFT
CCCEEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
ILDREDSKDLIKHCKAQLMEQRGNDGSLKLPSPGVIQSIFSYSVNCMVSLDESIAKHNRK
EEECCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHCCCEEEEEEHHHHHHCCCE
FFAVRDELEEIWKSYTQLKLEHNTLDYDDLLIKAVELFENNPEILIRESSRFQWILVDEF
EEHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECC
QDTNLVQYKLVEMLAGVHGNLIVVGDDAQSIYSFRGARFENVYDFLNRDQTSVFKIQTNY
CCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCC
RSTPEIVRLVNHLVPKSSLEKQLKAVRPSGPVPVIVETWDNLDEASFVAQKINEHIEDGI
CCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
QPNRIAVLYRSHFHSLELQLELDRRRVPYIVFSGPKFVETAHVKDILAFLKILQNPYDQL
CCCEEEEEEECCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHH
SWGRVLRLFPGVGIRRATQIINAIRESLGNDNEVISALRPFATPRVRIERLISLLEELNS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
EQSPEEVISTIYKGFYGEYMDEHFSDAKERRMDIERLIEVADRYDSIGEFLEDLAVSEKV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHC
DIEREIVDREEKIVLSTVHQAKGLEWDVVFILAMNPGDFPHALAIMEGNLDEEERIFYVA
CHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCEEEEE
VTRARDYLYLIRQRTGRSRPMIGNSYVFRSGHDFLTKIPEDVAERWDVGWNI
EEHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHCHHHHHHHCCCCCCC
>Mature Secondary Structure
MKRYNLKPHGIPNFIQESLDNEQLNAVLNSKGRTLIVAGPGSGKTRVITFKIAYLVSSGV
CCCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEEEHHHHCCC
NPQNILLVTFTRAASREMIERARRVSGSDLRGMLAGTFHHVCNHFLRKYATHLGISSDFT
CCCEEEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
ILDREDSKDLIKHCKAQLMEQRGNDGSLKLPSPGVIQSIFSYSVNCMVSLDESIAKHNRK
EEECCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHCCCEEEEEEHHHHHHCCCE
FFAVRDELEEIWKSYTQLKLEHNTLDYDDLLIKAVELFENNPEILIRESSRFQWILVDEF
EEHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECC
QDTNLVQYKLVEMLAGVHGNLIVVGDDAQSIYSFRGARFENVYDFLNRDQTSVFKIQTNY
CCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCC
RSTPEIVRLVNHLVPKSSLEKQLKAVRPSGPVPVIVETWDNLDEASFVAQKINEHIEDGI
CCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
QPNRIAVLYRSHFHSLELQLELDRRRVPYIVFSGPKFVETAHVKDILAFLKILQNPYDQL
CCCEEEEEEECCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHH
SWGRVLRLFPGVGIRRATQIINAIRESLGNDNEVISALRPFATPRVRIERLISLLEELNS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
EQSPEEVISTIYKGFYGEYMDEHFSDAKERRMDIERLIEVADRYDSIGEFLEDLAVSEKV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHC
DIEREIVDREEKIVLSTVHQAKGLEWDVVFILAMNPGDFPHALAIMEGNLDEEERIFYVA
CHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCEEEEE
VTRARDYLYLIRQRTGRSRPMIGNSYVFRSGHDFLTKIPEDVAERWDVGWNI
EEHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]