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The map label for this gene is argG [H]

Identifier: 239616502

GI number: 239616502

Start: 106424

End: 107710

Strand: Direct

Name: argG [H]

Synonym: Kole_0092

Alternate gene names: 239616502

Gene position: 106424-107710 (Clockwise)

Preceding gene: 239616501

Following gene: 239616503

Centisome position: 4.62

GC content: 42.19

Gene sequence:

>1287_bases
ATGAAAAAAGAAAAAGTTATTCTTGCGTACAGTGGCGGACTGGATACTTCTGTAATCCTGAAATGGCTTTCAGAGAAAGG
CTTCGAAGTCATTGCTTTTGTTGCAAACGTGGGGCAAAGGGACGATTTCGACGCAATCAAGGAAAAAGCCCTGAAAACCG
GTGCTAAAAAAGTGTATGTTGAAGACTTACGAAAAGAATTTGTCACAGATTTTATCTTTCCAGCTTTGATGGGAAATGCA
ATTTATGAAGGAAGATACCTCCTTGGAACTTCTCTTGCCAGACCTTTAATAGCCAAGAAACAGGTCGAAATCGCCGAAAA
AGAAGGCGCGCAATACGTCGCCCACGGGGCCACCGGCAAGGGGAACGATCAGGTTAGGTTTGAGCTCAATTACGCTGCAT
TGAATCCACATTTGAAAATAATATCTCCCTGGAAGGATCCGGAGTTTCTTGAAAAATTCAAAGGCAGAACAGACCTGATA
AATTACGCAAAAGAAATGGGAATACCTATAACGGTGAGTGAGGAAAAGCCTTACAGTGAAGATGAAAATCTGATGCACAT
ATCACATGAGGCAGGAAAGCTTGAAGATCCAATGTACATACCGGAAGAACATATCTTCAATTGGACAGTCTCTCCAAAGG
ACGCTCCGGATGAGGAAACGCTTCTTGAGATACTCTTCAAAAATGGAATACCTACAAAGGTAGTCAATCTCGATGATGGA
ACTGAAAAAACAGATCCATTGGAACTTTTTGAGTATCTGAACGAAGTAGGTGCCAAAAACGGAGTTGGAAGGGTTGACAT
GATAGAAAACAGGTTCATAGGGATAAAATCCCGAGGCGTGTATGAAACACCGGGTGCAACTATCCTCTGGATCGCTCATA
GAGATCTTGAAGGTATAGCCATGGATAAAGAAGTGCTTCATCTCAGAGATATGTTGACTCCAAAATTTTCCGAGTTGATT
TACAACGGGTTCTGGTTCTCTCCAGAGATAGACTTCCTGTTGTCCGCATTTAAAAAAGCCCAGGAAGCGGTGGACGGAAA
AGTTGTCGTTTCAATATACAAAGGAAACGTGACACCGGTTTCAAGATATTCCCCCACTTCCCTTTACGACCAGGCGCTTT
CAAGTATGGATGTTGAAGGCGGGTTCAATGCAATTGATTCTAAAGGCTTTATAAACATACACGCTCTCAGGCTTAAAGCT
CATAATCTTGTTTTAAAAGGAAAAGACCCCTTCGCCTGGAGAAAGGAGATTGGAAATGTCTATAAAACTCTGGGAAAAGG
GATATGA

Upstream 100 bases:

>100_bases
TGAACGCCCACACGGCCATATCGTTTTGATATGGCCGTTTTTTTTTTATACATTCTAAGTCTTCAACATAAACAACCTAA
AAATATTGGAGGTGTTGCAT

Downstream 100 bases:

>100_bases
AGTTGATCCCATAATAGAAGCATTTACAATTGGAAACGACTACAAAACGGACATGAGGCTAATAAAATACGATATAAAAG
CCTCTATGGCCCATGCAGAA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKKEKVILAYSGGLDTSVILKWLSEKGFEVIAFVANVGQRDDFDAIKEKALKTGAKKVYVEDLRKEFVTDFIFPALMGNA
IYEGRYLLGTSLARPLIAKKQVEIAEKEGAQYVAHGATGKGNDQVRFELNYAALNPHLKIISPWKDPEFLEKFKGRTDLI
NYAKEMGIPITVSEEKPYSEDENLMHISHEAGKLEDPMYIPEEHIFNWTVSPKDAPDEETLLEILFKNGIPTKVVNLDDG
TEKTDPLELFEYLNEVGAKNGVGRVDMIENRFIGIKSRGVYETPGATILWIAHRDLEGIAMDKEVLHLRDMLTPKFSELI
YNGFWFSPEIDFLLSAFKKAQEAVDGKVVVSIYKGNVTPVSRYSPTSLYDQALSSMDVEGGFNAIDSKGFINIHALRLKA
HNLVLKGKDPFAWRKEIGNVYKTLGKGI

Sequences:

>Translated_428_residues
MKKEKVILAYSGGLDTSVILKWLSEKGFEVIAFVANVGQRDDFDAIKEKALKTGAKKVYVEDLRKEFVTDFIFPALMGNA
IYEGRYLLGTSLARPLIAKKQVEIAEKEGAQYVAHGATGKGNDQVRFELNYAALNPHLKIISPWKDPEFLEKFKGRTDLI
NYAKEMGIPITVSEEKPYSEDENLMHISHEAGKLEDPMYIPEEHIFNWTVSPKDAPDEETLLEILFKNGIPTKVVNLDDG
TEKTDPLELFEYLNEVGAKNGVGRVDMIENRFIGIKSRGVYETPGATILWIAHRDLEGIAMDKEVLHLRDMLTPKFSELI
YNGFWFSPEIDFLLSAFKKAQEAVDGKVVVSIYKGNVTPVSRYSPTSLYDQALSSMDVEGGFNAIDSKGFINIHALRLKA
HNLVLKGKDPFAWRKEIGNVYKTLGKGI
>Mature_428_residues
MKKEKVILAYSGGLDTSVILKWLSEKGFEVIAFVANVGQRDDFDAIKEKALKTGAKKVYVEDLRKEFVTDFIFPALMGNA
IYEGRYLLGTSLARPLIAKKQVEIAEKEGAQYVAHGATGKGNDQVRFELNYAALNPHLKIISPWKDPEFLEKFKGRTDLI
NYAKEMGIPITVSEEKPYSEDENLMHISHEAGKLEDPMYIPEEHIFNWTVSPKDAPDEETLLEILFKNGIPTKVVNLDDG
TEKTDPLELFEYLNEVGAKNGVGRVDMIENRFIGIKSRGVYETPGATILWIAHRDLEGIAMDKEVLHLRDMLTPKFSELI
YNGFWFSPEIDFLLSAFKKAQEAVDGKVVVSIYKGNVTPVSRYSPTSLYDQALSSMDVEGGFNAIDSKGFINIHALRLKA
HNLVLKGKDPFAWRKEIGNVYKTLGKGI

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=400, Percent_Identity=57.25, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI16950633, Length=400, Percent_Identity=57.25, Blast_Score=470, Evalue=1e-132,
Organism=Escherichia coli, GI1789563, Length=364, Percent_Identity=31.043956043956, Blast_Score=169, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6324514, Length=407, Percent_Identity=52.3341523341523, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI21358151, Length=405, Percent_Identity=50.6172839506173, Blast_Score=424, Evalue=1e-119,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 48102; Mature: 48102

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEKVILAYSGGLDTSVILKWLSEKGFEVIAFVANVGQRDDFDAIKEKALKTGAKKVYV
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEHHCCCCCCHHHHHHHHHHHCCHHHHH
EDLRKEFVTDFIFPALMGNAIYEGRYLLGTSLARPLIAKKQVEIAEKEGAQYVAHGATGK
HHHHHHHHHHHHHHHHHCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
GNDQVRFELNYAALNPHLKIISPWKDPEFLEKFKGRTDLINYAKEMGIPITVSEEKPYSE
CCCEEEEEEEEEEECCCEEEECCCCCHHHHHHHCCHHHHHHHHHHCCCCEEECCCCCCCC
DENLMHISHEAGKLEDPMYIPEEHIFNWTVSPKDAPDEETLLEILFKNGIPTKVVNLDDG
CCCEEEEECCCCCCCCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCC
TEKTDPLELFEYLNEVGAKNGVGRVDMIENRFIGIKSRGVYETPGATILWIAHRDLEGIA
CCCCCHHHHHHHHHHHCCCCCCCCEEECCCCEEEEECCCCEECCCCEEEEEEECCCCCCC
MDKEVLHLRDMLTPKFSELIYNGFWFSPEIDFLLSAFKKAQEAVDGKVVVSIYKGNVTPV
CCHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
SRYSPTSLYDQALSSMDVEGGFNAIDSKGFINIHALRLKAHNLVLKGKDPFAWRKEIGNV
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEECCCCCHHHHHHHHH
YKTLGKGI
HHHHCCCC
>Mature Secondary Structure
MKKEKVILAYSGGLDTSVILKWLSEKGFEVIAFVANVGQRDDFDAIKEKALKTGAKKVYV
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEHHCCCCCCHHHHHHHHHHHCCHHHHH
EDLRKEFVTDFIFPALMGNAIYEGRYLLGTSLARPLIAKKQVEIAEKEGAQYVAHGATGK
HHHHHHHHHHHHHHHHHCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
GNDQVRFELNYAALNPHLKIISPWKDPEFLEKFKGRTDLINYAKEMGIPITVSEEKPYSE
CCCEEEEEEEEEEECCCEEEECCCCCHHHHHHHCCHHHHHHHHHHCCCCEEECCCCCCCC
DENLMHISHEAGKLEDPMYIPEEHIFNWTVSPKDAPDEETLLEILFKNGIPTKVVNLDDG
CCCEEEEECCCCCCCCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCC
TEKTDPLELFEYLNEVGAKNGVGRVDMIENRFIGIKSRGVYETPGATILWIAHRDLEGIA
CCCCCHHHHHHHHHHHCCCCCCCCEEECCCCEEEEECCCCEECCCCEEEEEEECCCCCCC
MDKEVLHLRDMLTPKFSELIYNGFWFSPEIDFLLSAFKKAQEAVDGKVVVSIYKGNVTPV
CCHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
SRYSPTSLYDQALSSMDVEGGFNAIDSKGFINIHALRLKAHNLVLKGKDPFAWRKEIGNV
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEECCCCCHHHHHHHHH
YKTLGKGI
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]