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The map label for this gene is hrb [H]

Identifier: 239616501

GI number: 239616501

Start: 105188

End: 105706

Strand: Direct

Name: hrb [H]

Synonym: Kole_0091

Alternate gene names: 239616501

Gene position: 105188-105706 (Clockwise)

Preceding gene: 239616500

Following gene: 239616502

Centisome position: 4.57

GC content: 41.62

Gene sequence:

>519_bases
ATGGATTATGAGGCTTTGTTTAAGGTCACTTATGGCATGTACGTGATTACTTCGAAGACGAATGAGAAGAAAAACGGGCA
GATTGCTAATGTTGTGTTCCAGGTTGTAGCTGAACCGCCAACAATAGCGATCTGCATAAATAAACAGAACTTAACCCATG
ACTTCATCCAAGAAAGCAGAGTATTTGGAGTGTCAGTTCTATCCCAGGATACCCCATTAAAACTAATAGGACACTTTGGA
TTTAGGTCCGGAAGAGAGTTAGACAAGTTCAAAGAAATAAATCACAAAATAGGGGTAACCGGTGTCCCGTTGTTGGAAGA
CTACTGCATAGCGAACTTAGAAGCGAAAGTGGTAAACGTAGTTGATGTTGGAACCCATGTGCTTTATATTGGAGAAATCG
TTAATGCGGAAGTTGTCGATGAAAAAGAACCCATGACATATGCCTATTATCATGAAATTAAGGGAGGAAGATCCCCGAAG
ACTGCTCCGACATATGCAGACGGAGGCTCACAACGTTGA

Upstream 100 bases:

>100_bases
GGAGGATTTTTATGTCAAAGGTAATGATGATGTAGAATGGTAAATGAGGTGTTGGAGAACCAAGGCTTTTTATTTTAATA
CCTGCTACGGAGGGGAAAAT

Downstream 100 bases:

>100_bases
GGCTTGCGGAGGCTGGCATTTGATAATACGACGCTTGCAGAGGCTATCAGCTGGTAGAGCGACGTTCGAAGACTGAGGCT
TGCGGACGCTGATGAAATCC

Product: flavin reductase domain protein FMN-binding

Products: NAD(+); reduced flavin [C]

Alternate protein names: Nitric oxide reductase NADH:FprA oxidoreductase [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MDYEALFKVTYGMYVITSKTNEKKNGQIANVVFQVVAEPPTIAICINKQNLTHDFIQESRVFGVSVLSQDTPLKLIGHFG
FRSGRELDKFKEINHKIGVTGVPLLEDYCIANLEAKVVNVVDVGTHVLYIGEIVNAEVVDEKEPMTYAYYHEIKGGRSPK
TAPTYADGGSQR

Sequences:

>Translated_172_residues
MDYEALFKVTYGMYVITSKTNEKKNGQIANVVFQVVAEPPTIAICINKQNLTHDFIQESRVFGVSVLSQDTPLKLIGHFG
FRSGRELDKFKEINHKIGVTGVPLLEDYCIANLEAKVVNVVDVGTHVLYIGEIVNAEVVDEKEPMTYAYYHEIKGGRSPK
TAPTYADGGSQR
>Mature_172_residues
MDYEALFKVTYGMYVITSKTNEKKNGQIANVVFQVVAEPPTIAICINKQNLTHDFIQESRVFGVSVLSQDTPLKLIGHFG
FRSGRELDKFKEINHKIGVTGVPLLEDYCIANLEAKVVNVVDVGTHVLYIGEIVNAEVVDEKEPMTYAYYHEIKGGRSPK
TAPTYADGGSQR

Specific function: Has nitric oxide reductase activity in combination with FprA; probably involved in nitrosative stress protection. Acts as an NADH:FprA oxidoreductase [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rubredoxin-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR004039
- InterPro:   IPR018527
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct; PF00301 Rubredoxin [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 19141; Mature: 19141

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYEALFKVTYGMYVITSKTNEKKNGQIANVVFQVVAEPPTIAICINKQNLTHDFIQESR
CCHHHHEEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHCC
VFGVSVLSQDTPLKLIGHFGFRSGRELDKFKEINHKIGVTGVPLLEDYCIANLEAKVVNV
EEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEE
VDVGTHVLYIGEIVNAEVVDEKEPMTYAYYHEIKGGRSPKTAPTYADGGSQR
EECCCEEEEEECCCCCEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDYEALFKVTYGMYVITSKTNEKKNGQIANVVFQVVAEPPTIAICINKQNLTHDFIQESR
CCHHHHEEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHCC
VFGVSVLSQDTPLKLIGHFGFRSGRELDKFKEINHKIGVTGVPLLEDYCIANLEAKVVNV
EEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEE
VDVGTHVLYIGEIVNAEVVDEKEPMTYAYYHEIKGGRSPKTAPTYADGGSQR
EECCCEEEEEECCCCCEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11160086; 12627946 [H]