Click here to switch to the map view.

The map label for this gene is trpB [H]

Identifier: 238925321

GI number: 238925321

Start: 2858843

End: 2860033

Strand: Reverse

Name: trpB [H]

Synonym: EUBREC_2976

Alternate gene names: 238925321

Gene position: 2860033-2858843 (Counterclockwise)

Preceding gene: 238925322

Following gene: 238925320

Centisome position: 82.91

GC content: 47.44

Gene sequence:

>1191_bases
ATGACAAATCCAAACGGAAGATTTGGTATTCACGGAGGACAGTACATTCCTGAAACCCTCATGAATGCCGTTAAAGAATT
AGAAGAAGCATACAATTATTACAAAAACGATCCTGAATTTAACAGAGAGCTCACAGAGCTTCTCAACGATTATGCAGGAA
GACCATCGAGACTGTACTACGCAGAAAAGATGACAAAGGATCTCGGTGGAGCAAAAATATACCTTAAAAGAGAGGATTTA
AACCATACAGGAGCCCACAAGATCAACAACGTATTAGGTCAGGCACTTCTTGCAAAGAAAATGGGCAAAACCCGCCTGAT
AGCTGAGACCGGAGCCGGTCAGCACGGTGTGGCAACAGCAACGGCCGCAGCCCTTATGGGTATGGAATGCGTTGTATTCA
TGGGCGAGGAGGACACAAAGCGTCAGGCGCTCAATGTATACCGCATGCGTCTGCTCGGAGCAGAGGTTATCCCGGTTACA
TCAGGTACAGCCACATTAAAGGATGCCGTATCGGAGGCAATGAGAGAGTGGACCAAGAGAATTTCGGACACACATTACTG
CCTGGGCTCAGTTATGGGACCACATCCGTTCCCTACCATCGTAAGAGATTTCCAGGCGGTCATCTCAAAGGAAATCAAGG
AGCAGATGCTTGAAAAGGAGCACAGACTGCCTGATGCGGTTATCGCATGTGTGGGCGGCGGCTCAAATGCCATTGGAAGC
TTTTACAACTTCATAGAGGATGAGGGAGTTGAGCTCATCGGCTGCGAGGCAGCAGGAAGGGGAATAGATACATTTGAGAC
TGCAGCCACAGTAAATACAGGCCGTCTTGGTATCTTCCACGGCATGAAGTCATATTTCTGTCAGGATAAATATGGACAGA
TTGCTCCGGTTTATTCAATTTCTGCAGGACTTGACTATCCGGGAGTCGGACCGGAGCACGCATATCTGCACGATATCGGA
AGAGCAAAATATGTACCGGTAACAGATGATGAAGCGGTAAATGCTTTTGAATACCTCGCAAAGACAGAGGGAATCATCCC
TGCAATCGAGTCCTCACATGCAGTGGCATATGCCATGAAGCTGGCGCCTACAATGGACAAGGACAAGATAATCGTTGTTA
CTGTTTCAGGCAGAGGCGACAAGGACTGCGCGGCAATAGCAAGATACAGAGGGGAGAATATTTATGAATAA

Upstream 100 bases:

>100_bases
AATAGAGATATATAAGTATCTTCATGGTTTTAAGAATATCTGTAACTATTCAGTACAGAAAACAAAATATTTACAAATAT
ACATCAGAAGGGAAAACATC

Downstream 100 bases:

>100_bases
GATAAGAAGTGCGTTTGAAAACGGCAAGGCGTTTATACCATTTATCACCTGTGGTGATCCAAATTTAGAGACAACTGAAA
AAATCGTAAGAGAAGCAGTT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTNPNGRFGIHGGQYIPETLMNAVKELEEAYNYYKNDPEFNRELTELLNDYAGRPSRLYYAEKMTKDLGGAKIYLKREDL
NHTGAHKINNVLGQALLAKKMGKTRLIAETGAGQHGVATATAAALMGMECVVFMGEEDTKRQALNVYRMRLLGAEVIPVT
SGTATLKDAVSEAMREWTKRISDTHYCLGSVMGPHPFPTIVRDFQAVISKEIKEQMLEKEHRLPDAVIACVGGGSNAIGS
FYNFIEDEGVELIGCEAAGRGIDTFETAATVNTGRLGIFHGMKSYFCQDKYGQIAPVYSISAGLDYPGVGPEHAYLHDIG
RAKYVPVTDDEAVNAFEYLAKTEGIIPAIESSHAVAYAMKLAPTMDKDKIIVVTVSGRGDKDCAAIARYRGENIYE

Sequences:

>Translated_396_residues
MTNPNGRFGIHGGQYIPETLMNAVKELEEAYNYYKNDPEFNRELTELLNDYAGRPSRLYYAEKMTKDLGGAKIYLKREDL
NHTGAHKINNVLGQALLAKKMGKTRLIAETGAGQHGVATATAAALMGMECVVFMGEEDTKRQALNVYRMRLLGAEVIPVT
SGTATLKDAVSEAMREWTKRISDTHYCLGSVMGPHPFPTIVRDFQAVISKEIKEQMLEKEHRLPDAVIACVGGGSNAIGS
FYNFIEDEGVELIGCEAAGRGIDTFETAATVNTGRLGIFHGMKSYFCQDKYGQIAPVYSISAGLDYPGVGPEHAYLHDIG
RAKYVPVTDDEAVNAFEYLAKTEGIIPAIESSHAVAYAMKLAPTMDKDKIIVVTVSGRGDKDCAAIARYRGENIYE
>Mature_395_residues
TNPNGRFGIHGGQYIPETLMNAVKELEEAYNYYKNDPEFNRELTELLNDYAGRPSRLYYAEKMTKDLGGAKIYLKREDLN
HTGAHKINNVLGQALLAKKMGKTRLIAETGAGQHGVATATAAALMGMECVVFMGEEDTKRQALNVYRMRLLGAEVIPVTS
GTATLKDAVSEAMREWTKRISDTHYCLGSVMGPHPFPTIVRDFQAVISKEIKEQMLEKEHRLPDAVIACVGGGSNAIGSF
YNFIEDEGVELIGCEAAGRGIDTFETAATVNTGRLGIFHGMKSYFCQDKYGQIAPVYSISAGLDYPGVGPEHAYLHDIGR
AKYVPVTDDEAVNAFEYLAKTEGIIPAIESSHAVAYAMKLAPTMDKDKIIVVTVSGRGDKDCAAIARYRGENIYE

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=392, Percent_Identity=57.1428571428571, Blast_Score=450, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6321412, Length=382, Percent_Identity=56.282722513089, Blast_Score=444, Evalue=1e-125,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 43402; Mature: 43271

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPNGRFGIHGGQYIPETLMNAVKELEEAYNYYKNDPEFNRELTELLNDYAGRPSRLYY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEHH
AEKMTKDLGGAKIYLKREDLNHTGAHKINNVLGQALLAKKMGKTRLIAETGAGQHGVATA
HHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
TAAALMGMECVVFMGEEDTKRQALNVYRMRLLGAEVIPVTSGTATLKDAVSEAMREWTKR
HHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHH
ISDTHYCLGSVMGPHPFPTIVRDFQAVISKEIKEQMLEKEHRLPDAVIACVGGGSNAIGS
HCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
FYNFIEDEGVELIGCEAAGRGIDTFETAATVNTGRLGIFHGMKSYFCQDKYGQIAPVYSI
HHHHHHHCCCEEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEE
SAGLDYPGVGPEHAYLHDIGRAKYVPVTDDEAVNAFEYLAKTEGIIPAIESSHAVAYAMK
ECCCCCCCCCCCHHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LAPTMDKDKIIVVTVSGRGDKDCAAIARYRGENIYE
HCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TNPNGRFGIHGGQYIPETLMNAVKELEEAYNYYKNDPEFNRELTELLNDYAGRPSRLYY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEHH
AEKMTKDLGGAKIYLKREDLNHTGAHKINNVLGQALLAKKMGKTRLIAETGAGQHGVATA
HHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
TAAALMGMECVVFMGEEDTKRQALNVYRMRLLGAEVIPVTSGTATLKDAVSEAMREWTKR
HHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHH
ISDTHYCLGSVMGPHPFPTIVRDFQAVISKEIKEQMLEKEHRLPDAVIACVGGGSNAIGS
HCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
FYNFIEDEGVELIGCEAAGRGIDTFETAATVNTGRLGIFHGMKSYFCQDKYGQIAPVYSI
HHHHHHHCCCEEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEE
SAGLDYPGVGPEHAYLHDIGRAKYVPVTDDEAVNAFEYLAKTEGIIPAIESSHAVAYAMK
ECCCCCCCCCCCHHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LAPTMDKDKIIVVTVSGRGDKDCAAIARYRGENIYE
HCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]