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The map label for this gene is trpA [H]

Identifier: 238925320

GI number: 238925320

Start: 2858083

End: 2858850

Strand: Reverse

Name: trpA [H]

Synonym: EUBREC_2975

Alternate gene names: 238925320

Gene position: 2858850-2858083 (Counterclockwise)

Preceding gene: 238925321

Following gene: 238925319

Centisome position: 82.87

GC content: 43.36

Gene sequence:

>768_bases
ATGAATAAGATAAGAAGTGCGTTTGAAAACGGCAAGGCGTTTATACCATTTATCACCTGTGGTGATCCAAATTTAGAGAC
AACTGAAAAAATCGTAAGAGAAGCAGTTGCAAACGGAGCAGACCTTATAGAGCTGGGAATTCCATTCTCTGATCCTACAG
CAGAGGGACCTGTAATCCAGGGCGCCAACATAAAGGCACTTGCAGCCGGAGTTACAACAGACAAGGTTTTTGACCTTGTG
AGAGATCTGAGAAAAGATGTCACAATTCCTATGGTTTTCATGACATATGCAAACGTGGTTTACTCTTACGGCGCAGAAAA
GTTTATCAGTACATGTAAGGAAATAGGCATTGACGGACTCATTCTGCCGGATATCCCATATGAGGAAAAGGAAGAGTTTT
TACCACTCTGCGAGAAATATGATGTGGACCTTATTTCATTTATCGCACCTACATCAAAGGATCGCATTGCGATGATAGCA
AAGGAGGCAACCGGCTTCATCTATGTGATTTCAAGCCTCGGAGTCACCGGTACAAGAAGCGAGATTAAGACAGATCTGGC
TTCAATAGTCAAGGTAATCAGAGAAAACACAGATGTGCCATGTGCTATCGGTTTTGGCATTTCAACACCCAAGCAGGCAA
AGAAGATGGCAGATATCTCGGATGGTGCCATTGTGGGCTCTGCCATCATCAAGATAATCGATGAGTATGGTGAGGCTTCA
CCAAAGTATGTCGGTGAGTATGTTAAAGCAATGAAGGATGCACTGTAG

Upstream 100 bases:

>100_bases
GCGCCTACAATGGACAAGGACAAGATAATCGTTGTTACTGTTTCAGGCAGAGGCGACAAGGACTGCGCGGCAATAGCAAG
ATACAGAGGGGAGAATATTT

Downstream 100 bases:

>100_bases
AGGCATTGTATTTTTATGTATGTAAAGGAGATTTTCTAAGGAAATTTAAAATAATATTATAAAATCAGAAAAAATGACTT
TTATACCCATATTTCAAAAA

Product: tryptophan synthase, alpha subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNKIRSAFENGKAFIPFITCGDPNLETTEKIVREAVANGADLIELGIPFSDPTAEGPVIQGANIKALAAGVTTDKVFDLV
RDLRKDVTIPMVFMTYANVVYSYGAEKFISTCKEIGIDGLILPDIPYEEKEEFLPLCEKYDVDLISFIAPTSKDRIAMIA
KEATGFIYVISSLGVTGTRSEIKTDLASIVKVIRENTDVPCAIGFGISTPKQAKKMADISDGAIVGSAIIKIIDEYGEAS
PKYVGEYVKAMKDAL

Sequences:

>Translated_255_residues
MNKIRSAFENGKAFIPFITCGDPNLETTEKIVREAVANGADLIELGIPFSDPTAEGPVIQGANIKALAAGVTTDKVFDLV
RDLRKDVTIPMVFMTYANVVYSYGAEKFISTCKEIGIDGLILPDIPYEEKEEFLPLCEKYDVDLISFIAPTSKDRIAMIA
KEATGFIYVISSLGVTGTRSEIKTDLASIVKVIRENTDVPCAIGFGISTPKQAKKMADISDGAIVGSAIIKIIDEYGEAS
PKYVGEYVKAMKDAL
>Mature_255_residues
MNKIRSAFENGKAFIPFITCGDPNLETTEKIVREAVANGADLIELGIPFSDPTAEGPVIQGANIKALAAGVTTDKVFDLV
RDLRKDVTIPMVFMTYANVVYSYGAEKFISTCKEIGIDGLILPDIPYEEKEEFLPLCEKYDVDLISFIAPTSKDRIAMIA
KEATGFIYVISSLGVTGTRSEIKTDLASIVKVIRENTDVPCAIGFGISTPKQAKKMADISDGAIVGSAIIKIIDEYGEAS
PKYVGEYVKAMKDAL

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=266, Percent_Identity=37.218045112782, Blast_Score=167, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6321412, Length=258, Percent_Identity=34.8837209302326, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 27606; Mature: 27606

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIRSAFENGKAFIPFITCGDPNLETTEKIVREAVANGADLIELGIPFSDPTAEGPVIQ
CCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEE
GANIKALAAGVTTDKVFDLVRDLRKDVTIPMVFMTYANVVYSYGAEKFISTCKEIGIDGL
CCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCE
ILPDIPYEEKEEFLPLCEKYDVDLISFIAPTSKDRIAMIAKEATGFIYVISSLGVTGTRS
ECCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCHHEEHHHHHCCCEEEEECCCCCCCHH
EIKTDLASIVKVIRENTDVPCAIGFGISTPKQAKKMADISDGAIVGSAIIKIIDEYGEAS
HHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
PKYVGEYVKAMKDAL
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKIRSAFENGKAFIPFITCGDPNLETTEKIVREAVANGADLIELGIPFSDPTAEGPVIQ
CCHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEE
GANIKALAAGVTTDKVFDLVRDLRKDVTIPMVFMTYANVVYSYGAEKFISTCKEIGIDGL
CCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCE
ILPDIPYEEKEEFLPLCEKYDVDLISFIAPTSKDRIAMIAKEATGFIYVISSLGVTGTRS
ECCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCHHEEHHHHHCCCEEEEECCCCCCCHH
EIKTDLASIVKVIRENTDVPCAIGFGISTPKQAKKMADISDGAIVGSAIIKIIDEYGEAS
HHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
PKYVGEYVKAMKDAL
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113923 [H]